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Controllable pH Manipulations in Micro/Nanofluidic Device Using Nanoscale Electrokinetics

Recently introduced nanoscale electrokinetic phenomenon called ion concentration polarization (ICP) has been suffered from serious pH changes to the sample fluid. A number of studies have focused on the origin of pH changes and strategies for regulating it. Instead of avoiding pH changes, in this wo...

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Autores principales: Park, Jae Suk, Oh, Jeewhan, Kim, Sung Jae
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7231315/
https://www.ncbi.nlm.nih.gov/pubmed/32290354
http://dx.doi.org/10.3390/mi11040400
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author Park, Jae Suk
Oh, Jeewhan
Kim, Sung Jae
author_facet Park, Jae Suk
Oh, Jeewhan
Kim, Sung Jae
author_sort Park, Jae Suk
collection PubMed
description Recently introduced nanoscale electrokinetic phenomenon called ion concentration polarization (ICP) has been suffered from serious pH changes to the sample fluid. A number of studies have focused on the origin of pH changes and strategies for regulating it. Instead of avoiding pH changes, in this work, we tried to demonstrate new ways to utilize this inevitable pH change. First, one can obtain a well-defined pH gradient in proton-received microchannel by applying a fixed electric current through a proton exchange membrane. Furthermore, one can tune the pH gradient on demand by adjusting the proton mass transportation (i.e., adjusting electric current). Secondly, we demonstrated that the occurrence of ICP can be examined by sensing a surrounding pH of electrolyte solution. When pH > threshold pH, patterned pH-responsive hydrogel inside a straight microchannel acted as a nanojunction to block the microchannel, while it did as a microjunction when pH < threshold pH. In case of forming a nanojunction, electrical current significantly dropped compared to the case of a microjunction. The strategies that presented in this work would be a basis for useful engineering applications such as a localized pH stimulation to biomolecules using tunable pH gradient generation and portable pH sensor with pH-sensitive hydrogel.
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spelling pubmed-72313152020-05-22 Controllable pH Manipulations in Micro/Nanofluidic Device Using Nanoscale Electrokinetics Park, Jae Suk Oh, Jeewhan Kim, Sung Jae Micromachines (Basel) Article Recently introduced nanoscale electrokinetic phenomenon called ion concentration polarization (ICP) has been suffered from serious pH changes to the sample fluid. A number of studies have focused on the origin of pH changes and strategies for regulating it. Instead of avoiding pH changes, in this work, we tried to demonstrate new ways to utilize this inevitable pH change. First, one can obtain a well-defined pH gradient in proton-received microchannel by applying a fixed electric current through a proton exchange membrane. Furthermore, one can tune the pH gradient on demand by adjusting the proton mass transportation (i.e., adjusting electric current). Secondly, we demonstrated that the occurrence of ICP can be examined by sensing a surrounding pH of electrolyte solution. When pH > threshold pH, patterned pH-responsive hydrogel inside a straight microchannel acted as a nanojunction to block the microchannel, while it did as a microjunction when pH < threshold pH. In case of forming a nanojunction, electrical current significantly dropped compared to the case of a microjunction. The strategies that presented in this work would be a basis for useful engineering applications such as a localized pH stimulation to biomolecules using tunable pH gradient generation and portable pH sensor with pH-sensitive hydrogel. MDPI 2020-04-10 /pmc/articles/PMC7231315/ /pubmed/32290354 http://dx.doi.org/10.3390/mi11040400 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Park, Jae Suk
Oh, Jeewhan
Kim, Sung Jae
Controllable pH Manipulations in Micro/Nanofluidic Device Using Nanoscale Electrokinetics
title Controllable pH Manipulations in Micro/Nanofluidic Device Using Nanoscale Electrokinetics
title_full Controllable pH Manipulations in Micro/Nanofluidic Device Using Nanoscale Electrokinetics
title_fullStr Controllable pH Manipulations in Micro/Nanofluidic Device Using Nanoscale Electrokinetics
title_full_unstemmed Controllable pH Manipulations in Micro/Nanofluidic Device Using Nanoscale Electrokinetics
title_short Controllable pH Manipulations in Micro/Nanofluidic Device Using Nanoscale Electrokinetics
title_sort controllable ph manipulations in micro/nanofluidic device using nanoscale electrokinetics
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7231315/
https://www.ncbi.nlm.nih.gov/pubmed/32290354
http://dx.doi.org/10.3390/mi11040400
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