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Differential Sorting of Microparticles Using Spiral Microchannels with Elliptic Configurations

Label-free, size-dependent cell-sorting applications based on inertial focusing phenomena have attracted much interest during the last decade. The separation capability heavily depends on the precision of microparticle focusing. In this study, five-loop spiral microchannels with a height of 90 µm an...

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Autores principales: Erdem, Kaan, Ahmadi, Vahid Ebrahimpour, Kosar, Ali, Kuddusi, Lütfullah
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7231368/
https://www.ncbi.nlm.nih.gov/pubmed/32295138
http://dx.doi.org/10.3390/mi11040412
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author Erdem, Kaan
Ahmadi, Vahid Ebrahimpour
Kosar, Ali
Kuddusi, Lütfullah
author_facet Erdem, Kaan
Ahmadi, Vahid Ebrahimpour
Kosar, Ali
Kuddusi, Lütfullah
author_sort Erdem, Kaan
collection PubMed
description Label-free, size-dependent cell-sorting applications based on inertial focusing phenomena have attracted much interest during the last decade. The separation capability heavily depends on the precision of microparticle focusing. In this study, five-loop spiral microchannels with a height of 90 µm and a width of 500 µm are introduced. Unlike their original spiral counterparts, these channels have elliptic configurations of varying initial aspect ratios, namely major axis to minor axis ratios of 3:2, 11:9, 9:11, and 2:3. Accordingly, the curvature of these configurations increases in a curvilinear manner through the channel. The effects of the alternating curvature and channel Reynolds number on the focusing of fluorescent microparticles with sizes of 10 and 20 µm in the prepared suspensions were investigated. At volumetric flow rates between 0.5 and 3.5 mL/min (allowing separation), each channel was tested to collect samples at the designated outlets. Then, these samples were analyzed by counting the particles. These curved channels were capable of separating 20 and 10 µm particles with total yields up to approximately 95% and 90%, respectively. The results exhibited that the level of enrichment and the focusing behavior of the proposed configurations are promising compared to the existing microfluidic channel configurations.
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spelling pubmed-72313682020-05-22 Differential Sorting of Microparticles Using Spiral Microchannels with Elliptic Configurations Erdem, Kaan Ahmadi, Vahid Ebrahimpour Kosar, Ali Kuddusi, Lütfullah Micromachines (Basel) Article Label-free, size-dependent cell-sorting applications based on inertial focusing phenomena have attracted much interest during the last decade. The separation capability heavily depends on the precision of microparticle focusing. In this study, five-loop spiral microchannels with a height of 90 µm and a width of 500 µm are introduced. Unlike their original spiral counterparts, these channels have elliptic configurations of varying initial aspect ratios, namely major axis to minor axis ratios of 3:2, 11:9, 9:11, and 2:3. Accordingly, the curvature of these configurations increases in a curvilinear manner through the channel. The effects of the alternating curvature and channel Reynolds number on the focusing of fluorescent microparticles with sizes of 10 and 20 µm in the prepared suspensions were investigated. At volumetric flow rates between 0.5 and 3.5 mL/min (allowing separation), each channel was tested to collect samples at the designated outlets. Then, these samples were analyzed by counting the particles. These curved channels were capable of separating 20 and 10 µm particles with total yields up to approximately 95% and 90%, respectively. The results exhibited that the level of enrichment and the focusing behavior of the proposed configurations are promising compared to the existing microfluidic channel configurations. MDPI 2020-04-14 /pmc/articles/PMC7231368/ /pubmed/32295138 http://dx.doi.org/10.3390/mi11040412 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Erdem, Kaan
Ahmadi, Vahid Ebrahimpour
Kosar, Ali
Kuddusi, Lütfullah
Differential Sorting of Microparticles Using Spiral Microchannels with Elliptic Configurations
title Differential Sorting of Microparticles Using Spiral Microchannels with Elliptic Configurations
title_full Differential Sorting of Microparticles Using Spiral Microchannels with Elliptic Configurations
title_fullStr Differential Sorting of Microparticles Using Spiral Microchannels with Elliptic Configurations
title_full_unstemmed Differential Sorting of Microparticles Using Spiral Microchannels with Elliptic Configurations
title_short Differential Sorting of Microparticles Using Spiral Microchannels with Elliptic Configurations
title_sort differential sorting of microparticles using spiral microchannels with elliptic configurations
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7231368/
https://www.ncbi.nlm.nih.gov/pubmed/32295138
http://dx.doi.org/10.3390/mi11040412
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