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P16INK4A Immunohistochemistry as a Gold Standard for Cervical Cancer and Precursor Lesions Screening
BACKGROUND: High-risk (HR) Human papillomaviruses (HPVs) are known as the main factors implicated in the pathogenesis of cervical preinvasive and invasive lesions. Therefore, the presence or absence of HR-HPV can be followed for the prognosis of low-grade and high-grade squamous intraepithelial lesi...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Tehran University of Medical Sciences
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7231710/ https://www.ncbi.nlm.nih.gov/pubmed/32461939 |
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author | FARZANEHPOUR, Mahdieh MUHAMMADNEJAD, Ahad AKHAVAN, Setareh EMAMI RAZAVI, Amir Nader JALILVAND, Somayeh SALIMI, Vahid FAGHIHLOO, Ebrahim KAKAVANDI, Ehsan FARAHMAND, Mohammad SHAYESTEHPOUR, Mohammad BABAKHANI, Farzad MOKHTARI AZAD, Talat |
author_facet | FARZANEHPOUR, Mahdieh MUHAMMADNEJAD, Ahad AKHAVAN, Setareh EMAMI RAZAVI, Amir Nader JALILVAND, Somayeh SALIMI, Vahid FAGHIHLOO, Ebrahim KAKAVANDI, Ehsan FARAHMAND, Mohammad SHAYESTEHPOUR, Mohammad BABAKHANI, Farzad MOKHTARI AZAD, Talat |
author_sort | FARZANEHPOUR, Mahdieh |
collection | PubMed |
description | BACKGROUND: High-risk (HR) Human papillomaviruses (HPVs) are known as the main factors implicated in the pathogenesis of cervical preinvasive and invasive lesions. Therefore, the presence or absence of HR-HPV can be followed for the prognosis of low-grade and high-grade squamous intraepithelial lesions. Since the overexpression of p16INK4a protein depends on the presence of transcriptionally-active HPV, and due to its availability and simple interpretation, it may be considered as a proper marker to diagnose cervical cancer. METHODS: An immunohistochemical analysis of p16INK4a was performed in 72 cervical tissue specimens at Imam Khomeini Complex Hospital (Tehran, Iran) from 2016 to 2018. The performance parameters were calculated and compared using receiving operating characteristics curve (ROC) details. RESULTS: p16INK4a is significantly up-regulated in the cervical cancer samples in comparison with that in normal samples. Moreover, the ROC data showed the potential ability of p16INK4a under determined conditions as a diagnostic marker for CIN 2–3 staging and invasive cervical cancer. The molecular typing disclosed the attendance of HPV DNA in 44.4% of cases (32/72) with a predominance of HPV type 16. CONCLUSION: The molecular biomarker p16INK4a can be a good candidate for the early diagnosis and prognosis of cervical cancer in HPV-infected patients. Considering the increase in the expression level of p16INK4a in cancer and precancer tissues, p16INK4a may be used for early detection of cervical cancer. |
format | Online Article Text |
id | pubmed-7231710 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Tehran University of Medical Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-72317102020-05-26 P16INK4A Immunohistochemistry as a Gold Standard for Cervical Cancer and Precursor Lesions Screening FARZANEHPOUR, Mahdieh MUHAMMADNEJAD, Ahad AKHAVAN, Setareh EMAMI RAZAVI, Amir Nader JALILVAND, Somayeh SALIMI, Vahid FAGHIHLOO, Ebrahim KAKAVANDI, Ehsan FARAHMAND, Mohammad SHAYESTEHPOUR, Mohammad BABAKHANI, Farzad MOKHTARI AZAD, Talat Iran J Public Health Original Article BACKGROUND: High-risk (HR) Human papillomaviruses (HPVs) are known as the main factors implicated in the pathogenesis of cervical preinvasive and invasive lesions. Therefore, the presence or absence of HR-HPV can be followed for the prognosis of low-grade and high-grade squamous intraepithelial lesions. Since the overexpression of p16INK4a protein depends on the presence of transcriptionally-active HPV, and due to its availability and simple interpretation, it may be considered as a proper marker to diagnose cervical cancer. METHODS: An immunohistochemical analysis of p16INK4a was performed in 72 cervical tissue specimens at Imam Khomeini Complex Hospital (Tehran, Iran) from 2016 to 2018. The performance parameters were calculated and compared using receiving operating characteristics curve (ROC) details. RESULTS: p16INK4a is significantly up-regulated in the cervical cancer samples in comparison with that in normal samples. Moreover, the ROC data showed the potential ability of p16INK4a under determined conditions as a diagnostic marker for CIN 2–3 staging and invasive cervical cancer. The molecular typing disclosed the attendance of HPV DNA in 44.4% of cases (32/72) with a predominance of HPV type 16. CONCLUSION: The molecular biomarker p16INK4a can be a good candidate for the early diagnosis and prognosis of cervical cancer in HPV-infected patients. Considering the increase in the expression level of p16INK4a in cancer and precancer tissues, p16INK4a may be used for early detection of cervical cancer. Tehran University of Medical Sciences 2020-02 /pmc/articles/PMC7231710/ /pubmed/32461939 Text en Copyright © Iranian Public Health Association & Tehran University of Medical Sciences http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article FARZANEHPOUR, Mahdieh MUHAMMADNEJAD, Ahad AKHAVAN, Setareh EMAMI RAZAVI, Amir Nader JALILVAND, Somayeh SALIMI, Vahid FAGHIHLOO, Ebrahim KAKAVANDI, Ehsan FARAHMAND, Mohammad SHAYESTEHPOUR, Mohammad BABAKHANI, Farzad MOKHTARI AZAD, Talat P16INK4A Immunohistochemistry as a Gold Standard for Cervical Cancer and Precursor Lesions Screening |
title | P16INK4A Immunohistochemistry as a Gold Standard for Cervical Cancer and Precursor Lesions Screening |
title_full | P16INK4A Immunohistochemistry as a Gold Standard for Cervical Cancer and Precursor Lesions Screening |
title_fullStr | P16INK4A Immunohistochemistry as a Gold Standard for Cervical Cancer and Precursor Lesions Screening |
title_full_unstemmed | P16INK4A Immunohistochemistry as a Gold Standard for Cervical Cancer and Precursor Lesions Screening |
title_short | P16INK4A Immunohistochemistry as a Gold Standard for Cervical Cancer and Precursor Lesions Screening |
title_sort | p16ink4a immunohistochemistry as a gold standard for cervical cancer and precursor lesions screening |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7231710/ https://www.ncbi.nlm.nih.gov/pubmed/32461939 |
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