Effect of Chitosan Nanoparticle from Penaeus semisulcatus Shrimp on Salmonella typhi and Listeria monocytogenes

BACKGROUND: After cellulose, chitin is one of the most important polymers in crustaceans, insects, and fungi. Chitosan is one of the most important derivatives of chitin, which has important characteristics including degradability, non-toxicity, and biocompatibility antimicrobial and antioxidant properties. METHODS: Chitosan was extracted from Penaeus semisulcatus shrimp using chemical methods and the degree of its austenitization was determined using a sub-red spectrophotometer and XRD. The nanoparticles were then synthesized using the ionic gelation method and analyzed through SEM. The antimicrobial effects of nanoparticles were also evaluated using antimicrobial tests on Listeria monocytogenes and Salmonella typhi. RESULTS: Nanoparticles have antimicrobial activity and can inhibit bacterial growth at different concentrations. CONCLUSION: Chitosan nanoparticles have an inhibitory effect on Listeria monocytogenes, which is a gram-positive bacterium.