Multiplex PCR for simultaneous identification of the most common European Opisthorchiid and Heterophyid in fish or fish products

Among others, the families Opisthorchiidae and Heterophyidae includes several genera causing fish-borne zoonoses and distributed also in European Countries and that are included in the ParaFishControl (Advanced Tools and Research Strategies for Parasite Control in European farmed fish) H2020 EU proj...

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Autores principales: Caffara, Monica, Gustinelli, Andrea, Mazzone, Angelica, Fioravanti, Maria L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7232091/
https://www.ncbi.nlm.nih.gov/pubmed/32435707
http://dx.doi.org/10.1016/j.fawpar.2020.e00081
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author Caffara, Monica
Gustinelli, Andrea
Mazzone, Angelica
Fioravanti, Maria L.
author_facet Caffara, Monica
Gustinelli, Andrea
Mazzone, Angelica
Fioravanti, Maria L.
author_sort Caffara, Monica
collection PubMed
description Among others, the families Opisthorchiidae and Heterophyidae includes several genera causing fish-borne zoonoses and distributed also in European Countries and that are included in the ParaFishControl (Advanced Tools and Research Strategies for Parasite Control in European farmed fish) H2020 EU project. Due to the small size of the metacercariae, the infective stage for human, these parasites cannot be detected visually in fish and monitoring requires expert application of time-consuming techniques. The aim of this was to develop a rapid and affordable molecular method based on multiplex PCR for simultaneous identification of metacercariae of the most common European Opisthorchiid and Heterophyid in fish or fish products.
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spelling pubmed-72320912020-05-20 Multiplex PCR for simultaneous identification of the most common European Opisthorchiid and Heterophyid in fish or fish products Caffara, Monica Gustinelli, Andrea Mazzone, Angelica Fioravanti, Maria L. Food Waterborne Parasitol Short Communication Among others, the families Opisthorchiidae and Heterophyidae includes several genera causing fish-borne zoonoses and distributed also in European Countries and that are included in the ParaFishControl (Advanced Tools and Research Strategies for Parasite Control in European farmed fish) H2020 EU project. Due to the small size of the metacercariae, the infective stage for human, these parasites cannot be detected visually in fish and monitoring requires expert application of time-consuming techniques. The aim of this was to develop a rapid and affordable molecular method based on multiplex PCR for simultaneous identification of metacercariae of the most common European Opisthorchiid and Heterophyid in fish or fish products. Elsevier 2020-05-11 /pmc/articles/PMC7232091/ /pubmed/32435707 http://dx.doi.org/10.1016/j.fawpar.2020.e00081 Text en © 2020 The Authors. Published by Elsevier Inc. on behalf of International Association of Food and Waterborne Parasitology. http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Short Communication
Caffara, Monica
Gustinelli, Andrea
Mazzone, Angelica
Fioravanti, Maria L.
Multiplex PCR for simultaneous identification of the most common European Opisthorchiid and Heterophyid in fish or fish products
title Multiplex PCR for simultaneous identification of the most common European Opisthorchiid and Heterophyid in fish or fish products
title_full Multiplex PCR for simultaneous identification of the most common European Opisthorchiid and Heterophyid in fish or fish products
title_fullStr Multiplex PCR for simultaneous identification of the most common European Opisthorchiid and Heterophyid in fish or fish products
title_full_unstemmed Multiplex PCR for simultaneous identification of the most common European Opisthorchiid and Heterophyid in fish or fish products
title_short Multiplex PCR for simultaneous identification of the most common European Opisthorchiid and Heterophyid in fish or fish products
title_sort multiplex pcr for simultaneous identification of the most common european opisthorchiid and heterophyid in fish or fish products
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7232091/
https://www.ncbi.nlm.nih.gov/pubmed/32435707
http://dx.doi.org/10.1016/j.fawpar.2020.e00081
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