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Hyperandrogenism in POMCa-deficient zebrafish enhances somatic growth without increasing adiposity

The endocrine regulatory roles of the hypothalamic–pituitary–adrenocortical axis on anxiety-like behavior and metabolic status have been found throughout animal taxa. However, the precise effects of the balancing adrenal corticosteroid biosynthesis under the influence of adrenocorticotrophic hormone...

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Autores principales: Shi, Chuang, Lu, Yao, Zhai, Gang, Huang, Jianfei, Shang, Guohui, Lou, Qiyong, Li, Dongliang, Jin, Xia, He, Jiangyan, Du, Zhenyu, Gui, Jianfang, Yin, Zhan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7232124/
https://www.ncbi.nlm.nih.gov/pubmed/31237951
http://dx.doi.org/10.1093/jmcb/mjz053
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author Shi, Chuang
Lu, Yao
Zhai, Gang
Huang, Jianfei
Shang, Guohui
Lou, Qiyong
Li, Dongliang
Jin, Xia
He, Jiangyan
Du, Zhenyu
Gui, Jianfang
Yin, Zhan
author_facet Shi, Chuang
Lu, Yao
Zhai, Gang
Huang, Jianfei
Shang, Guohui
Lou, Qiyong
Li, Dongliang
Jin, Xia
He, Jiangyan
Du, Zhenyu
Gui, Jianfang
Yin, Zhan
author_sort Shi, Chuang
collection PubMed
description The endocrine regulatory roles of the hypothalamic–pituitary–adrenocortical axis on anxiety-like behavior and metabolic status have been found throughout animal taxa. However, the precise effects of the balancing adrenal corticosteroid biosynthesis under the influence of adrenocorticotrophic hormone (ACTH), a pro-opiomelanocortin (POMC)-derived peptide, on animal energy expenditure and somatic growth remain unknown. POMC has also been identified as one of the candidate loci for polycystic ovary syndrome, which features hyperandrogenism and some prevalence of obesity in patients. Here we show that zebrafish lacking functional POMCa exhibit similar phenotypes of stress response and body weight gain but not obesity as observed in mammalian models. In contrast with the impaired anorexigenic signaling cascade of melanocyte-stimulating hormones and leptin, which are responsible for their obesity-prone weight gain observed in various pomc mutant mammals, analyses with our pomca mutant series indicate that ACTH is the key regulator for the phenotype with enhanced somatic growth without obesity in pomca-deficient zebrafish. Hypocortisolism associated with hyperandrogenism has been observed in the pomca-deficient zebrafish, with enhanced activation of mammalian target of rapamycin complex 1; reutilization of amino acids and fatty acid β-oxidation are observed in the muscle tissue of the pomca-deficient fish. After reducing hyperandrogenism by crossing our pomca mutant fish with a cyp17a1-deficient background, the phenotype of enhanced somatic growth in pomca-deficient fish was no longer observed. Thus, our work also demonstrated that the role of POMCa in stress response seems to be conserved in vertebrates, whereas its effect on adipostasis is unique to teleosts.
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spelling pubmed-72321242020-05-21 Hyperandrogenism in POMCa-deficient zebrafish enhances somatic growth without increasing adiposity Shi, Chuang Lu, Yao Zhai, Gang Huang, Jianfei Shang, Guohui Lou, Qiyong Li, Dongliang Jin, Xia He, Jiangyan Du, Zhenyu Gui, Jianfang Yin, Zhan J Mol Cell Biol Article The endocrine regulatory roles of the hypothalamic–pituitary–adrenocortical axis on anxiety-like behavior and metabolic status have been found throughout animal taxa. However, the precise effects of the balancing adrenal corticosteroid biosynthesis under the influence of adrenocorticotrophic hormone (ACTH), a pro-opiomelanocortin (POMC)-derived peptide, on animal energy expenditure and somatic growth remain unknown. POMC has also been identified as one of the candidate loci for polycystic ovary syndrome, which features hyperandrogenism and some prevalence of obesity in patients. Here we show that zebrafish lacking functional POMCa exhibit similar phenotypes of stress response and body weight gain but not obesity as observed in mammalian models. In contrast with the impaired anorexigenic signaling cascade of melanocyte-stimulating hormones and leptin, which are responsible for their obesity-prone weight gain observed in various pomc mutant mammals, analyses with our pomca mutant series indicate that ACTH is the key regulator for the phenotype with enhanced somatic growth without obesity in pomca-deficient zebrafish. Hypocortisolism associated with hyperandrogenism has been observed in the pomca-deficient zebrafish, with enhanced activation of mammalian target of rapamycin complex 1; reutilization of amino acids and fatty acid β-oxidation are observed in the muscle tissue of the pomca-deficient fish. After reducing hyperandrogenism by crossing our pomca mutant fish with a cyp17a1-deficient background, the phenotype of enhanced somatic growth in pomca-deficient fish was no longer observed. Thus, our work also demonstrated that the role of POMCa in stress response seems to be conserved in vertebrates, whereas its effect on adipostasis is unique to teleosts. Oxford University Press 2019-06-25 /pmc/articles/PMC7232124/ /pubmed/31237951 http://dx.doi.org/10.1093/jmcb/mjz053 Text en © The Author(s) (2019). Published by Oxford University Press on behalf of Journal of Molecular Cell Biology, IBCB, SIBS, CAS. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Article
Shi, Chuang
Lu, Yao
Zhai, Gang
Huang, Jianfei
Shang, Guohui
Lou, Qiyong
Li, Dongliang
Jin, Xia
He, Jiangyan
Du, Zhenyu
Gui, Jianfang
Yin, Zhan
Hyperandrogenism in POMCa-deficient zebrafish enhances somatic growth without increasing adiposity
title Hyperandrogenism in POMCa-deficient zebrafish enhances somatic growth without increasing adiposity
title_full Hyperandrogenism in POMCa-deficient zebrafish enhances somatic growth without increasing adiposity
title_fullStr Hyperandrogenism in POMCa-deficient zebrafish enhances somatic growth without increasing adiposity
title_full_unstemmed Hyperandrogenism in POMCa-deficient zebrafish enhances somatic growth without increasing adiposity
title_short Hyperandrogenism in POMCa-deficient zebrafish enhances somatic growth without increasing adiposity
title_sort hyperandrogenism in pomca-deficient zebrafish enhances somatic growth without increasing adiposity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7232124/
https://www.ncbi.nlm.nih.gov/pubmed/31237951
http://dx.doi.org/10.1093/jmcb/mjz053
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