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Hyperandrogenism in POMCa-deficient zebrafish enhances somatic growth without increasing adiposity
The endocrine regulatory roles of the hypothalamic–pituitary–adrenocortical axis on anxiety-like behavior and metabolic status have been found throughout animal taxa. However, the precise effects of the balancing adrenal corticosteroid biosynthesis under the influence of adrenocorticotrophic hormone...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7232124/ https://www.ncbi.nlm.nih.gov/pubmed/31237951 http://dx.doi.org/10.1093/jmcb/mjz053 |
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author | Shi, Chuang Lu, Yao Zhai, Gang Huang, Jianfei Shang, Guohui Lou, Qiyong Li, Dongliang Jin, Xia He, Jiangyan Du, Zhenyu Gui, Jianfang Yin, Zhan |
author_facet | Shi, Chuang Lu, Yao Zhai, Gang Huang, Jianfei Shang, Guohui Lou, Qiyong Li, Dongliang Jin, Xia He, Jiangyan Du, Zhenyu Gui, Jianfang Yin, Zhan |
author_sort | Shi, Chuang |
collection | PubMed |
description | The endocrine regulatory roles of the hypothalamic–pituitary–adrenocortical axis on anxiety-like behavior and metabolic status have been found throughout animal taxa. However, the precise effects of the balancing adrenal corticosteroid biosynthesis under the influence of adrenocorticotrophic hormone (ACTH), a pro-opiomelanocortin (POMC)-derived peptide, on animal energy expenditure and somatic growth remain unknown. POMC has also been identified as one of the candidate loci for polycystic ovary syndrome, which features hyperandrogenism and some prevalence of obesity in patients. Here we show that zebrafish lacking functional POMCa exhibit similar phenotypes of stress response and body weight gain but not obesity as observed in mammalian models. In contrast with the impaired anorexigenic signaling cascade of melanocyte-stimulating hormones and leptin, which are responsible for their obesity-prone weight gain observed in various pomc mutant mammals, analyses with our pomca mutant series indicate that ACTH is the key regulator for the phenotype with enhanced somatic growth without obesity in pomca-deficient zebrafish. Hypocortisolism associated with hyperandrogenism has been observed in the pomca-deficient zebrafish, with enhanced activation of mammalian target of rapamycin complex 1; reutilization of amino acids and fatty acid β-oxidation are observed in the muscle tissue of the pomca-deficient fish. After reducing hyperandrogenism by crossing our pomca mutant fish with a cyp17a1-deficient background, the phenotype of enhanced somatic growth in pomca-deficient fish was no longer observed. Thus, our work also demonstrated that the role of POMCa in stress response seems to be conserved in vertebrates, whereas its effect on adipostasis is unique to teleosts. |
format | Online Article Text |
id | pubmed-7232124 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-72321242020-05-21 Hyperandrogenism in POMCa-deficient zebrafish enhances somatic growth without increasing adiposity Shi, Chuang Lu, Yao Zhai, Gang Huang, Jianfei Shang, Guohui Lou, Qiyong Li, Dongliang Jin, Xia He, Jiangyan Du, Zhenyu Gui, Jianfang Yin, Zhan J Mol Cell Biol Article The endocrine regulatory roles of the hypothalamic–pituitary–adrenocortical axis on anxiety-like behavior and metabolic status have been found throughout animal taxa. However, the precise effects of the balancing adrenal corticosteroid biosynthesis under the influence of adrenocorticotrophic hormone (ACTH), a pro-opiomelanocortin (POMC)-derived peptide, on animal energy expenditure and somatic growth remain unknown. POMC has also been identified as one of the candidate loci for polycystic ovary syndrome, which features hyperandrogenism and some prevalence of obesity in patients. Here we show that zebrafish lacking functional POMCa exhibit similar phenotypes of stress response and body weight gain but not obesity as observed in mammalian models. In contrast with the impaired anorexigenic signaling cascade of melanocyte-stimulating hormones and leptin, which are responsible for their obesity-prone weight gain observed in various pomc mutant mammals, analyses with our pomca mutant series indicate that ACTH is the key regulator for the phenotype with enhanced somatic growth without obesity in pomca-deficient zebrafish. Hypocortisolism associated with hyperandrogenism has been observed in the pomca-deficient zebrafish, with enhanced activation of mammalian target of rapamycin complex 1; reutilization of amino acids and fatty acid β-oxidation are observed in the muscle tissue of the pomca-deficient fish. After reducing hyperandrogenism by crossing our pomca mutant fish with a cyp17a1-deficient background, the phenotype of enhanced somatic growth in pomca-deficient fish was no longer observed. Thus, our work also demonstrated that the role of POMCa in stress response seems to be conserved in vertebrates, whereas its effect on adipostasis is unique to teleosts. Oxford University Press 2019-06-25 /pmc/articles/PMC7232124/ /pubmed/31237951 http://dx.doi.org/10.1093/jmcb/mjz053 Text en © The Author(s) (2019). Published by Oxford University Press on behalf of Journal of Molecular Cell Biology, IBCB, SIBS, CAS. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
spellingShingle | Article Shi, Chuang Lu, Yao Zhai, Gang Huang, Jianfei Shang, Guohui Lou, Qiyong Li, Dongliang Jin, Xia He, Jiangyan Du, Zhenyu Gui, Jianfang Yin, Zhan Hyperandrogenism in POMCa-deficient zebrafish enhances somatic growth without increasing adiposity |
title | Hyperandrogenism in POMCa-deficient zebrafish enhances somatic growth without increasing adiposity |
title_full | Hyperandrogenism in POMCa-deficient zebrafish enhances somatic growth without increasing adiposity |
title_fullStr | Hyperandrogenism in POMCa-deficient zebrafish enhances somatic growth without increasing adiposity |
title_full_unstemmed | Hyperandrogenism in POMCa-deficient zebrafish enhances somatic growth without increasing adiposity |
title_short | Hyperandrogenism in POMCa-deficient zebrafish enhances somatic growth without increasing adiposity |
title_sort | hyperandrogenism in pomca-deficient zebrafish enhances somatic growth without increasing adiposity |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7232124/ https://www.ncbi.nlm.nih.gov/pubmed/31237951 http://dx.doi.org/10.1093/jmcb/mjz053 |
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