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Evaluation of the Allplex(TM) Gastrointestinal Panel—Parasite Assay for Protozoa Detection in Stool Samples: A Retrospective and Prospective Study

This study aims at evaluating the performances of the multiplex PCR Allplex(TM) Gastrointestinal Panel-Parasite Assay (GIPPA), which detects G. duodenalis, Cryptosporidium spp., E. histolytica, D. fragilis, B. hominis, and C. cayetanensis, by comparison to microscopy. A retrospective evaluation was...

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Autores principales: Autier, Brice, Gangneux, Jean-Pierre, Robert-Gangneux, Florence
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7232139/
https://www.ncbi.nlm.nih.gov/pubmed/32326453
http://dx.doi.org/10.3390/microorganisms8040569
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author Autier, Brice
Gangneux, Jean-Pierre
Robert-Gangneux, Florence
author_facet Autier, Brice
Gangneux, Jean-Pierre
Robert-Gangneux, Florence
author_sort Autier, Brice
collection PubMed
description This study aims at evaluating the performances of the multiplex PCR Allplex(TM) Gastrointestinal Panel-Parasite Assay (GIPPA), which detects G. duodenalis, Cryptosporidium spp., E. histolytica, D. fragilis, B. hominis, and C. cayetanensis, by comparison to microscopy. A retrospective evaluation was conducted on a series of positive clinical samples (n = 99) stored at −80 °C or at +4 °C. A five-month prospective study was then conducted on all samples sent to our lab for parasite detection (n = 586). In the retrospective cohort, sensitivity was 81% for both G. duodenalis (26/32) and D. fragilis (21/26) and 100% for Cryptosporidium spp. (26/26, including 6 different species), B. hominis (26/26), and C. cayetanensis (4/4). During the prospective study, 95 samples were positive by microscopy and 207 by multiplex PCR assay. The molecular assay showed a significantly higher sensitivity of PCR, especially for G. duodenalis (100% vs. 60.7%, p < 0.01), D. fragilis (97.2% vs. 14.1%, p < 0.001), and B. hominis (99.4% vs. 44.2%, p < 0.001) but also for E. histolytica (100% vs. 50.0%). The sensitivity of the Allplex(TM) GIPPA on the first stool sample was equivalent to the sensitivity of microscopy on multiple stool samples but inferior to multiplex PCR on multiple stool samples. Taken together, the Allplex(TM) GIPPA is suitable for the routine detection of protozoa in fecal samples.
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spelling pubmed-72321392020-06-01 Evaluation of the Allplex(TM) Gastrointestinal Panel—Parasite Assay for Protozoa Detection in Stool Samples: A Retrospective and Prospective Study Autier, Brice Gangneux, Jean-Pierre Robert-Gangneux, Florence Microorganisms Article This study aims at evaluating the performances of the multiplex PCR Allplex(TM) Gastrointestinal Panel-Parasite Assay (GIPPA), which detects G. duodenalis, Cryptosporidium spp., E. histolytica, D. fragilis, B. hominis, and C. cayetanensis, by comparison to microscopy. A retrospective evaluation was conducted on a series of positive clinical samples (n = 99) stored at −80 °C or at +4 °C. A five-month prospective study was then conducted on all samples sent to our lab for parasite detection (n = 586). In the retrospective cohort, sensitivity was 81% for both G. duodenalis (26/32) and D. fragilis (21/26) and 100% for Cryptosporidium spp. (26/26, including 6 different species), B. hominis (26/26), and C. cayetanensis (4/4). During the prospective study, 95 samples were positive by microscopy and 207 by multiplex PCR assay. The molecular assay showed a significantly higher sensitivity of PCR, especially for G. duodenalis (100% vs. 60.7%, p < 0.01), D. fragilis (97.2% vs. 14.1%, p < 0.001), and B. hominis (99.4% vs. 44.2%, p < 0.001) but also for E. histolytica (100% vs. 50.0%). The sensitivity of the Allplex(TM) GIPPA on the first stool sample was equivalent to the sensitivity of microscopy on multiple stool samples but inferior to multiplex PCR on multiple stool samples. Taken together, the Allplex(TM) GIPPA is suitable for the routine detection of protozoa in fecal samples. MDPI 2020-04-15 /pmc/articles/PMC7232139/ /pubmed/32326453 http://dx.doi.org/10.3390/microorganisms8040569 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Autier, Brice
Gangneux, Jean-Pierre
Robert-Gangneux, Florence
Evaluation of the Allplex(TM) Gastrointestinal Panel—Parasite Assay for Protozoa Detection in Stool Samples: A Retrospective and Prospective Study
title Evaluation of the Allplex(TM) Gastrointestinal Panel—Parasite Assay for Protozoa Detection in Stool Samples: A Retrospective and Prospective Study
title_full Evaluation of the Allplex(TM) Gastrointestinal Panel—Parasite Assay for Protozoa Detection in Stool Samples: A Retrospective and Prospective Study
title_fullStr Evaluation of the Allplex(TM) Gastrointestinal Panel—Parasite Assay for Protozoa Detection in Stool Samples: A Retrospective and Prospective Study
title_full_unstemmed Evaluation of the Allplex(TM) Gastrointestinal Panel—Parasite Assay for Protozoa Detection in Stool Samples: A Retrospective and Prospective Study
title_short Evaluation of the Allplex(TM) Gastrointestinal Panel—Parasite Assay for Protozoa Detection in Stool Samples: A Retrospective and Prospective Study
title_sort evaluation of the allplex(tm) gastrointestinal panel—parasite assay for protozoa detection in stool samples: a retrospective and prospective study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7232139/
https://www.ncbi.nlm.nih.gov/pubmed/32326453
http://dx.doi.org/10.3390/microorganisms8040569
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