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The Not so Good, the Bad and the Ugly: Differential Bacterial Adhesion and Invasion Mediated by Salmonella PagN Allelic Variants

While advances in genomic sequencing have highlighted significant strain variability between and within Salmonella serovars, only a few protein variants have been directly related to evolutionary adaptation for survival, such as host specificity or differential virulence. The current study investiga...

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Autores principales: Wu, Yanping, Hu, Qiaoyun, Dehinwal, Ruchika, Rakov, Alexey V., Grams, Nicholas, Clemens, Erin C., Hofmann, Jennifer, Okeke, Iruka N., Schifferli, Dieter M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7232170/
https://www.ncbi.nlm.nih.gov/pubmed/32235448
http://dx.doi.org/10.3390/microorganisms8040489
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author Wu, Yanping
Hu, Qiaoyun
Dehinwal, Ruchika
Rakov, Alexey V.
Grams, Nicholas
Clemens, Erin C.
Hofmann, Jennifer
Okeke, Iruka N.
Schifferli, Dieter M.
author_facet Wu, Yanping
Hu, Qiaoyun
Dehinwal, Ruchika
Rakov, Alexey V.
Grams, Nicholas
Clemens, Erin C.
Hofmann, Jennifer
Okeke, Iruka N.
Schifferli, Dieter M.
author_sort Wu, Yanping
collection PubMed
description While advances in genomic sequencing have highlighted significant strain variability between and within Salmonella serovars, only a few protein variants have been directly related to evolutionary adaptation for survival, such as host specificity or differential virulence. The current study investigated whether allelic variation of the Salmonella adhesin/invasin PagN influences bacterial interaction with their receptors. The Salmonella enterica, subspecies enterica serovar Typhi (S. Typhi) allelic variant of PagN was found to bind significantly better to different enterocytes as well as to the extracellular matrix protein laminin than did the major Salmonella enterica, subspecies enterica serovar Typhimurium (S. Typhimurium) allele. The two alleles differed at amino acid residues 49 and 109 in two of the four predicted PagN surface loops, and residue substitution analysis revealed that a glutamic acid at residue 49 increased the adhesive and invasive properties of S. Typhi PagN. PagN sequence comparisons from 542 Salmonella strains for six representative S. enterica serovars and S. diarizonae further supported the role of glutamic acid at residues 49 and 109 in optimizing adhesion to cells and laminin, as well as for cell invasion. In summary, this study characterized unique residues in allelic variants of a virulence factor that participates in the colonization and invasive properties of different Salmonella stains, subspecies and serovars.
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spelling pubmed-72321702020-05-22 The Not so Good, the Bad and the Ugly: Differential Bacterial Adhesion and Invasion Mediated by Salmonella PagN Allelic Variants Wu, Yanping Hu, Qiaoyun Dehinwal, Ruchika Rakov, Alexey V. Grams, Nicholas Clemens, Erin C. Hofmann, Jennifer Okeke, Iruka N. Schifferli, Dieter M. Microorganisms Article While advances in genomic sequencing have highlighted significant strain variability between and within Salmonella serovars, only a few protein variants have been directly related to evolutionary adaptation for survival, such as host specificity or differential virulence. The current study investigated whether allelic variation of the Salmonella adhesin/invasin PagN influences bacterial interaction with their receptors. The Salmonella enterica, subspecies enterica serovar Typhi (S. Typhi) allelic variant of PagN was found to bind significantly better to different enterocytes as well as to the extracellular matrix protein laminin than did the major Salmonella enterica, subspecies enterica serovar Typhimurium (S. Typhimurium) allele. The two alleles differed at amino acid residues 49 and 109 in two of the four predicted PagN surface loops, and residue substitution analysis revealed that a glutamic acid at residue 49 increased the adhesive and invasive properties of S. Typhi PagN. PagN sequence comparisons from 542 Salmonella strains for six representative S. enterica serovars and S. diarizonae further supported the role of glutamic acid at residues 49 and 109 in optimizing adhesion to cells and laminin, as well as for cell invasion. In summary, this study characterized unique residues in allelic variants of a virulence factor that participates in the colonization and invasive properties of different Salmonella stains, subspecies and serovars. MDPI 2020-03-30 /pmc/articles/PMC7232170/ /pubmed/32235448 http://dx.doi.org/10.3390/microorganisms8040489 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wu, Yanping
Hu, Qiaoyun
Dehinwal, Ruchika
Rakov, Alexey V.
Grams, Nicholas
Clemens, Erin C.
Hofmann, Jennifer
Okeke, Iruka N.
Schifferli, Dieter M.
The Not so Good, the Bad and the Ugly: Differential Bacterial Adhesion and Invasion Mediated by Salmonella PagN Allelic Variants
title The Not so Good, the Bad and the Ugly: Differential Bacterial Adhesion and Invasion Mediated by Salmonella PagN Allelic Variants
title_full The Not so Good, the Bad and the Ugly: Differential Bacterial Adhesion and Invasion Mediated by Salmonella PagN Allelic Variants
title_fullStr The Not so Good, the Bad and the Ugly: Differential Bacterial Adhesion and Invasion Mediated by Salmonella PagN Allelic Variants
title_full_unstemmed The Not so Good, the Bad and the Ugly: Differential Bacterial Adhesion and Invasion Mediated by Salmonella PagN Allelic Variants
title_short The Not so Good, the Bad and the Ugly: Differential Bacterial Adhesion and Invasion Mediated by Salmonella PagN Allelic Variants
title_sort not so good, the bad and the ugly: differential bacterial adhesion and invasion mediated by salmonella pagn allelic variants
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7232170/
https://www.ncbi.nlm.nih.gov/pubmed/32235448
http://dx.doi.org/10.3390/microorganisms8040489
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