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Recent Advances in Strategies for Activation and Discovery/Characterization of Cryptic Biosynthetic Gene Clusters in Streptomyces

Streptomyces spp. are prolific sources of valuable natural products (NPs) that are of great interest in pharmaceutical industries such as antibiotics, anticancer chemotherapeutics, immunosuppressants, etc. Approximately two-thirds of all known antibiotics are produced by actinomycetes, most predomin...

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Autores principales: Nguyen, Chung Thanh, Dhakal, Dipesh, Pham, Van Thuy Thi, Nguyen, Hue Thi, Sohng, Jae-Kyung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7232178/
https://www.ncbi.nlm.nih.gov/pubmed/32344564
http://dx.doi.org/10.3390/microorganisms8040616
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author Nguyen, Chung Thanh
Dhakal, Dipesh
Pham, Van Thuy Thi
Nguyen, Hue Thi
Sohng, Jae-Kyung
author_facet Nguyen, Chung Thanh
Dhakal, Dipesh
Pham, Van Thuy Thi
Nguyen, Hue Thi
Sohng, Jae-Kyung
author_sort Nguyen, Chung Thanh
collection PubMed
description Streptomyces spp. are prolific sources of valuable natural products (NPs) that are of great interest in pharmaceutical industries such as antibiotics, anticancer chemotherapeutics, immunosuppressants, etc. Approximately two-thirds of all known antibiotics are produced by actinomycetes, most predominantly by Streptomyces. Nevertheless, in recent years, the chances of the discovery of novel and bioactive compounds from Streptomyces have significantly declined. The major hindrance for obtaining such bioactive compounds from Streptomyces is that most of the compounds are not produced in significant titers, or the biosynthetic gene clusters (BGCs) are cryptic. The rapid development of genome sequencing has provided access to a tremendous number of NP-BGCs embedded in the microbial genomes. In addition, the studies of metabolomics provide a portfolio of entire metabolites produced from the strain of interest. Therefore, through the integrated approaches of different-omics techniques, the connection between gene expression and metabolism can be established. Hence, in this review we summarized recent advancements in strategies for activating cryptic BGCs in Streptomyces by utilizing diverse state-of-the-art techniques.
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spelling pubmed-72321782020-05-22 Recent Advances in Strategies for Activation and Discovery/Characterization of Cryptic Biosynthetic Gene Clusters in Streptomyces Nguyen, Chung Thanh Dhakal, Dipesh Pham, Van Thuy Thi Nguyen, Hue Thi Sohng, Jae-Kyung Microorganisms Review Streptomyces spp. are prolific sources of valuable natural products (NPs) that are of great interest in pharmaceutical industries such as antibiotics, anticancer chemotherapeutics, immunosuppressants, etc. Approximately two-thirds of all known antibiotics are produced by actinomycetes, most predominantly by Streptomyces. Nevertheless, in recent years, the chances of the discovery of novel and bioactive compounds from Streptomyces have significantly declined. The major hindrance for obtaining such bioactive compounds from Streptomyces is that most of the compounds are not produced in significant titers, or the biosynthetic gene clusters (BGCs) are cryptic. The rapid development of genome sequencing has provided access to a tremendous number of NP-BGCs embedded in the microbial genomes. In addition, the studies of metabolomics provide a portfolio of entire metabolites produced from the strain of interest. Therefore, through the integrated approaches of different-omics techniques, the connection between gene expression and metabolism can be established. Hence, in this review we summarized recent advancements in strategies for activating cryptic BGCs in Streptomyces by utilizing diverse state-of-the-art techniques. MDPI 2020-04-24 /pmc/articles/PMC7232178/ /pubmed/32344564 http://dx.doi.org/10.3390/microorganisms8040616 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Nguyen, Chung Thanh
Dhakal, Dipesh
Pham, Van Thuy Thi
Nguyen, Hue Thi
Sohng, Jae-Kyung
Recent Advances in Strategies for Activation and Discovery/Characterization of Cryptic Biosynthetic Gene Clusters in Streptomyces
title Recent Advances in Strategies for Activation and Discovery/Characterization of Cryptic Biosynthetic Gene Clusters in Streptomyces
title_full Recent Advances in Strategies for Activation and Discovery/Characterization of Cryptic Biosynthetic Gene Clusters in Streptomyces
title_fullStr Recent Advances in Strategies for Activation and Discovery/Characterization of Cryptic Biosynthetic Gene Clusters in Streptomyces
title_full_unstemmed Recent Advances in Strategies for Activation and Discovery/Characterization of Cryptic Biosynthetic Gene Clusters in Streptomyces
title_short Recent Advances in Strategies for Activation and Discovery/Characterization of Cryptic Biosynthetic Gene Clusters in Streptomyces
title_sort recent advances in strategies for activation and discovery/characterization of cryptic biosynthetic gene clusters in streptomyces
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7232178/
https://www.ncbi.nlm.nih.gov/pubmed/32344564
http://dx.doi.org/10.3390/microorganisms8040616
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