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Characterisation of Peste Des Petits Ruminants Disease in Pastoralist Flocks in Ngorongoro District of Northern Tanzania and Bluetongue Virus Co-Infection

Peste des petits ruminants (PPR) disease was first confirmed in Tanzania in 2008 in sheep and goats in Ngorongoro District, northern Tanzania, and is now endemic in this area. This study aimed to characterise PPR disease in pastoralist small ruminant flocks in Ngorongoro District. During June 2015,...

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Autores principales: Jones, Bryony Anne, Mahapatra, Mana, Chubwa, Chobi, Clarke, Brian, Batten, Carrie, Hicks, Hayley, Henstock, Mark, Keyyu, Julius, Kock, Richard, Parida, Satya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7232183/
https://www.ncbi.nlm.nih.gov/pubmed/32244509
http://dx.doi.org/10.3390/v12040389
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author Jones, Bryony Anne
Mahapatra, Mana
Chubwa, Chobi
Clarke, Brian
Batten, Carrie
Hicks, Hayley
Henstock, Mark
Keyyu, Julius
Kock, Richard
Parida, Satya
author_facet Jones, Bryony Anne
Mahapatra, Mana
Chubwa, Chobi
Clarke, Brian
Batten, Carrie
Hicks, Hayley
Henstock, Mark
Keyyu, Julius
Kock, Richard
Parida, Satya
author_sort Jones, Bryony Anne
collection PubMed
description Peste des petits ruminants (PPR) disease was first confirmed in Tanzania in 2008 in sheep and goats in Ngorongoro District, northern Tanzania, and is now endemic in this area. This study aimed to characterise PPR disease in pastoralist small ruminant flocks in Ngorongoro District. During June 2015, 33 PPR-like disease reports were investigated in different parts of the district, using semi-structured interviews, clinical examinations, PPR virus rapid detection test (PPRV-RDT), and laboratory analysis. Ten flocks were confirmed as PPRV infected by PPRV-RDT and/or real-time reverse transcription-polymerase chain reaction (RT-qPCR), and two flocks were co-infected with bluetongue virus (BTV), confirmed by RT-qPCR. Phylogenetic analysis of six partial N gene sequences showed that the PPR viruses clustered with recent lineage III Tanzanian viruses, and grouped with Ugandan, Kenyan and Democratic Republic of Congo isolates. No PPR-like disease was reported in wildlife. There was considerable variation in clinical syndromes between flocks: some showed a full range of PPR signs, while others were predominantly respiratory, diarrhoea, or oro-nasal syndromes, which were associated with different local disease names (olodua—a term for rinderpest, olkipiei—lung disease, oloirobi—fever, enkorotik—diarrhoea). BTV co-infection was associated with severe oro-nasal lesions. This clinical variability makes the field diagnosis of PPR challenging, highlighting the importance of access to pen-side antigen tests and multiplex assays to support improved surveillance and targeting of control activities for PPR eradication.
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spelling pubmed-72321832020-05-22 Characterisation of Peste Des Petits Ruminants Disease in Pastoralist Flocks in Ngorongoro District of Northern Tanzania and Bluetongue Virus Co-Infection Jones, Bryony Anne Mahapatra, Mana Chubwa, Chobi Clarke, Brian Batten, Carrie Hicks, Hayley Henstock, Mark Keyyu, Julius Kock, Richard Parida, Satya Viruses Article Peste des petits ruminants (PPR) disease was first confirmed in Tanzania in 2008 in sheep and goats in Ngorongoro District, northern Tanzania, and is now endemic in this area. This study aimed to characterise PPR disease in pastoralist small ruminant flocks in Ngorongoro District. During June 2015, 33 PPR-like disease reports were investigated in different parts of the district, using semi-structured interviews, clinical examinations, PPR virus rapid detection test (PPRV-RDT), and laboratory analysis. Ten flocks were confirmed as PPRV infected by PPRV-RDT and/or real-time reverse transcription-polymerase chain reaction (RT-qPCR), and two flocks were co-infected with bluetongue virus (BTV), confirmed by RT-qPCR. Phylogenetic analysis of six partial N gene sequences showed that the PPR viruses clustered with recent lineage III Tanzanian viruses, and grouped with Ugandan, Kenyan and Democratic Republic of Congo isolates. No PPR-like disease was reported in wildlife. There was considerable variation in clinical syndromes between flocks: some showed a full range of PPR signs, while others were predominantly respiratory, diarrhoea, or oro-nasal syndromes, which were associated with different local disease names (olodua—a term for rinderpest, olkipiei—lung disease, oloirobi—fever, enkorotik—diarrhoea). BTV co-infection was associated with severe oro-nasal lesions. This clinical variability makes the field diagnosis of PPR challenging, highlighting the importance of access to pen-side antigen tests and multiplex assays to support improved surveillance and targeting of control activities for PPR eradication. MDPI 2020-03-31 /pmc/articles/PMC7232183/ /pubmed/32244509 http://dx.doi.org/10.3390/v12040389 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Jones, Bryony Anne
Mahapatra, Mana
Chubwa, Chobi
Clarke, Brian
Batten, Carrie
Hicks, Hayley
Henstock, Mark
Keyyu, Julius
Kock, Richard
Parida, Satya
Characterisation of Peste Des Petits Ruminants Disease in Pastoralist Flocks in Ngorongoro District of Northern Tanzania and Bluetongue Virus Co-Infection
title Characterisation of Peste Des Petits Ruminants Disease in Pastoralist Flocks in Ngorongoro District of Northern Tanzania and Bluetongue Virus Co-Infection
title_full Characterisation of Peste Des Petits Ruminants Disease in Pastoralist Flocks in Ngorongoro District of Northern Tanzania and Bluetongue Virus Co-Infection
title_fullStr Characterisation of Peste Des Petits Ruminants Disease in Pastoralist Flocks in Ngorongoro District of Northern Tanzania and Bluetongue Virus Co-Infection
title_full_unstemmed Characterisation of Peste Des Petits Ruminants Disease in Pastoralist Flocks in Ngorongoro District of Northern Tanzania and Bluetongue Virus Co-Infection
title_short Characterisation of Peste Des Petits Ruminants Disease in Pastoralist Flocks in Ngorongoro District of Northern Tanzania and Bluetongue Virus Co-Infection
title_sort characterisation of peste des petits ruminants disease in pastoralist flocks in ngorongoro district of northern tanzania and bluetongue virus co-infection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7232183/
https://www.ncbi.nlm.nih.gov/pubmed/32244509
http://dx.doi.org/10.3390/v12040389
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