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The Availability of Iron Is Involved in the Murine Experimental Toxoplasma gondii Infection Outcome

Iron is an important constituent of our environment, being necessary for both mammalian and pathogenic protozoa survival. Iron-containing proteins exert a wide range of biological processes such as biodegradation and biosynthesis, as well as immune function, fetal development, and physical and menta...

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Autores principales: Oliveira, Mário Cézar, Coutinho, Loyane Bertagnolli, Almeida, Marcos Paulo Oliveira, Briceño, Marisol Pallete, Araujo, Ester Cristina Borges, Silva, Neide Maria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7232304/
https://www.ncbi.nlm.nih.gov/pubmed/32295126
http://dx.doi.org/10.3390/microorganisms8040560
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author Oliveira, Mário Cézar
Coutinho, Loyane Bertagnolli
Almeida, Marcos Paulo Oliveira
Briceño, Marisol Pallete
Araujo, Ester Cristina Borges
Silva, Neide Maria
author_facet Oliveira, Mário Cézar
Coutinho, Loyane Bertagnolli
Almeida, Marcos Paulo Oliveira
Briceño, Marisol Pallete
Araujo, Ester Cristina Borges
Silva, Neide Maria
author_sort Oliveira, Mário Cézar
collection PubMed
description Iron is an important constituent of our environment, being necessary for both mammalian and pathogenic protozoa survival. Iron-containing proteins exert a wide range of biological processes such as biodegradation and biosynthesis, as well as immune function, fetal development, and physical and mental well-being. This work aimed to investigate the effect of iron deprivation in Toxoplasma gondii infection outcome. C57BL/6 mice were orally infected with T. gondii and treated with an iron chelator, deferoxamine, or supplemented with iron (ferrous sulfate), and the parasitism as well as immunological and histological parameters were analyzed. It was observed that the infection increased iron accumulation in the organs, as well as systemically, and deferoxamine treatment diminished the iron content in serum samples and intestine. The deferoxamine treatment decreased the parasitism and inflammatory alterations in the small intestine and lung. Additionally, they partially preserved the Paneth cells and decreased the intestinal dysbiosis. The ferrous sulfate supplementation, despite not significantly increasing the parasite load in the organs, increased the inflammatory alterations in the liver. Together, our results suggest that iron chelation, which is commonly used to treat iron overload, could be a promising medicine to control T. gondii proliferation, mainly in the small intestine, and consequently inflammation caused by infection.
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spelling pubmed-72323042020-05-22 The Availability of Iron Is Involved in the Murine Experimental Toxoplasma gondii Infection Outcome Oliveira, Mário Cézar Coutinho, Loyane Bertagnolli Almeida, Marcos Paulo Oliveira Briceño, Marisol Pallete Araujo, Ester Cristina Borges Silva, Neide Maria Microorganisms Article Iron is an important constituent of our environment, being necessary for both mammalian and pathogenic protozoa survival. Iron-containing proteins exert a wide range of biological processes such as biodegradation and biosynthesis, as well as immune function, fetal development, and physical and mental well-being. This work aimed to investigate the effect of iron deprivation in Toxoplasma gondii infection outcome. C57BL/6 mice were orally infected with T. gondii and treated with an iron chelator, deferoxamine, or supplemented with iron (ferrous sulfate), and the parasitism as well as immunological and histological parameters were analyzed. It was observed that the infection increased iron accumulation in the organs, as well as systemically, and deferoxamine treatment diminished the iron content in serum samples and intestine. The deferoxamine treatment decreased the parasitism and inflammatory alterations in the small intestine and lung. Additionally, they partially preserved the Paneth cells and decreased the intestinal dysbiosis. The ferrous sulfate supplementation, despite not significantly increasing the parasite load in the organs, increased the inflammatory alterations in the liver. Together, our results suggest that iron chelation, which is commonly used to treat iron overload, could be a promising medicine to control T. gondii proliferation, mainly in the small intestine, and consequently inflammation caused by infection. MDPI 2020-04-14 /pmc/articles/PMC7232304/ /pubmed/32295126 http://dx.doi.org/10.3390/microorganisms8040560 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Oliveira, Mário Cézar
Coutinho, Loyane Bertagnolli
Almeida, Marcos Paulo Oliveira
Briceño, Marisol Pallete
Araujo, Ester Cristina Borges
Silva, Neide Maria
The Availability of Iron Is Involved in the Murine Experimental Toxoplasma gondii Infection Outcome
title The Availability of Iron Is Involved in the Murine Experimental Toxoplasma gondii Infection Outcome
title_full The Availability of Iron Is Involved in the Murine Experimental Toxoplasma gondii Infection Outcome
title_fullStr The Availability of Iron Is Involved in the Murine Experimental Toxoplasma gondii Infection Outcome
title_full_unstemmed The Availability of Iron Is Involved in the Murine Experimental Toxoplasma gondii Infection Outcome
title_short The Availability of Iron Is Involved in the Murine Experimental Toxoplasma gondii Infection Outcome
title_sort availability of iron is involved in the murine experimental toxoplasma gondii infection outcome
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7232304/
https://www.ncbi.nlm.nih.gov/pubmed/32295126
http://dx.doi.org/10.3390/microorganisms8040560
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