Cargando…

Retrieval of the Complete Coding Sequence of the UK-Endemic Tatenale Orthohantavirus Reveals Extensive Strain Variation and Supports Its Classification as a Novel Species

Orthohantaviruses are globally distributed viruses, associated with rodents and other small mammals. However, data on the circulation of orthohantaviruses within the UK, particularly the UK-endemic Tatenale virus, is sparse. In this study, 531 animals from five rodent species were collected from two...

Descripción completa

Detalles Bibliográficos
Autores principales: Chappell, Joseph G., Tsoleridis, Theocharis, Onianwa, Okechukwu, Drake, Gabby, Ashpole, Ian, Dobbs, Phillipa, Edema, William, Kumi-Ansah, Frederick, Bennett, Malcolm, Tarlinton, Rachael E., Ball, Jonathan K., McClure, C. Patrick
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7232349/
https://www.ncbi.nlm.nih.gov/pubmed/32316655
http://dx.doi.org/10.3390/v12040454
_version_ 1783535367544111104
author Chappell, Joseph G.
Tsoleridis, Theocharis
Onianwa, Okechukwu
Drake, Gabby
Ashpole, Ian
Dobbs, Phillipa
Edema, William
Kumi-Ansah, Frederick
Bennett, Malcolm
Tarlinton, Rachael E.
Ball, Jonathan K.
McClure, C. Patrick
author_facet Chappell, Joseph G.
Tsoleridis, Theocharis
Onianwa, Okechukwu
Drake, Gabby
Ashpole, Ian
Dobbs, Phillipa
Edema, William
Kumi-Ansah, Frederick
Bennett, Malcolm
Tarlinton, Rachael E.
Ball, Jonathan K.
McClure, C. Patrick
author_sort Chappell, Joseph G.
collection PubMed
description Orthohantaviruses are globally distributed viruses, associated with rodents and other small mammals. However, data on the circulation of orthohantaviruses within the UK, particularly the UK-endemic Tatenale virus, is sparse. In this study, 531 animals from five rodent species were collected from two locations in northern and central England and screened using a degenerate, pan- orthohantavirus RT-PCR assay. Tatenale virus was detected in a single field vole (Microtus agrestis) from central England and twelve field voles from northern England. Unbiased high-throughput sequencing of the central English strain resulted in the recovery of the complete coding sequence of a novel strain of Tatenale virus, whilst PCR-primer walking of the northern English strain recovered almost complete coding sequence of a previously identified strain. These findings represented the detection of a third lineage of Tatenale virus in the United Kingdom and extended the known geographic distribution of these viruses from northern to central England. Furthermore, the recovery of the complete coding sequence revealed that Tatenale virus was sufficiently related to the recently identified Traemersee virus, to meet the accepted criteria for classification as a single species of orthohantavirus.
format Online
Article
Text
id pubmed-7232349
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-72323492020-05-22 Retrieval of the Complete Coding Sequence of the UK-Endemic Tatenale Orthohantavirus Reveals Extensive Strain Variation and Supports Its Classification as a Novel Species Chappell, Joseph G. Tsoleridis, Theocharis Onianwa, Okechukwu Drake, Gabby Ashpole, Ian Dobbs, Phillipa Edema, William Kumi-Ansah, Frederick Bennett, Malcolm Tarlinton, Rachael E. Ball, Jonathan K. McClure, C. Patrick Viruses Communication Orthohantaviruses are globally distributed viruses, associated with rodents and other small mammals. However, data on the circulation of orthohantaviruses within the UK, particularly the UK-endemic Tatenale virus, is sparse. In this study, 531 animals from five rodent species were collected from two locations in northern and central England and screened using a degenerate, pan- orthohantavirus RT-PCR assay. Tatenale virus was detected in a single field vole (Microtus agrestis) from central England and twelve field voles from northern England. Unbiased high-throughput sequencing of the central English strain resulted in the recovery of the complete coding sequence of a novel strain of Tatenale virus, whilst PCR-primer walking of the northern English strain recovered almost complete coding sequence of a previously identified strain. These findings represented the detection of a third lineage of Tatenale virus in the United Kingdom and extended the known geographic distribution of these viruses from northern to central England. Furthermore, the recovery of the complete coding sequence revealed that Tatenale virus was sufficiently related to the recently identified Traemersee virus, to meet the accepted criteria for classification as a single species of orthohantavirus. MDPI 2020-04-17 /pmc/articles/PMC7232349/ /pubmed/32316655 http://dx.doi.org/10.3390/v12040454 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Chappell, Joseph G.
Tsoleridis, Theocharis
Onianwa, Okechukwu
Drake, Gabby
Ashpole, Ian
Dobbs, Phillipa
Edema, William
Kumi-Ansah, Frederick
Bennett, Malcolm
Tarlinton, Rachael E.
Ball, Jonathan K.
McClure, C. Patrick
Retrieval of the Complete Coding Sequence of the UK-Endemic Tatenale Orthohantavirus Reveals Extensive Strain Variation and Supports Its Classification as a Novel Species
title Retrieval of the Complete Coding Sequence of the UK-Endemic Tatenale Orthohantavirus Reveals Extensive Strain Variation and Supports Its Classification as a Novel Species
title_full Retrieval of the Complete Coding Sequence of the UK-Endemic Tatenale Orthohantavirus Reveals Extensive Strain Variation and Supports Its Classification as a Novel Species
title_fullStr Retrieval of the Complete Coding Sequence of the UK-Endemic Tatenale Orthohantavirus Reveals Extensive Strain Variation and Supports Its Classification as a Novel Species
title_full_unstemmed Retrieval of the Complete Coding Sequence of the UK-Endemic Tatenale Orthohantavirus Reveals Extensive Strain Variation and Supports Its Classification as a Novel Species
title_short Retrieval of the Complete Coding Sequence of the UK-Endemic Tatenale Orthohantavirus Reveals Extensive Strain Variation and Supports Its Classification as a Novel Species
title_sort retrieval of the complete coding sequence of the uk-endemic tatenale orthohantavirus reveals extensive strain variation and supports its classification as a novel species
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7232349/
https://www.ncbi.nlm.nih.gov/pubmed/32316655
http://dx.doi.org/10.3390/v12040454
work_keys_str_mv AT chappelljosephg retrievalofthecompletecodingsequenceoftheukendemictatenaleorthohantavirusrevealsextensivestrainvariationandsupportsitsclassificationasanovelspecies
AT tsoleridistheocharis retrievalofthecompletecodingsequenceoftheukendemictatenaleorthohantavirusrevealsextensivestrainvariationandsupportsitsclassificationasanovelspecies
AT onianwaokechukwu retrievalofthecompletecodingsequenceoftheukendemictatenaleorthohantavirusrevealsextensivestrainvariationandsupportsitsclassificationasanovelspecies
AT drakegabby retrievalofthecompletecodingsequenceoftheukendemictatenaleorthohantavirusrevealsextensivestrainvariationandsupportsitsclassificationasanovelspecies
AT ashpoleian retrievalofthecompletecodingsequenceoftheukendemictatenaleorthohantavirusrevealsextensivestrainvariationandsupportsitsclassificationasanovelspecies
AT dobbsphillipa retrievalofthecompletecodingsequenceoftheukendemictatenaleorthohantavirusrevealsextensivestrainvariationandsupportsitsclassificationasanovelspecies
AT edemawilliam retrievalofthecompletecodingsequenceoftheukendemictatenaleorthohantavirusrevealsextensivestrainvariationandsupportsitsclassificationasanovelspecies
AT kumiansahfrederick retrievalofthecompletecodingsequenceoftheukendemictatenaleorthohantavirusrevealsextensivestrainvariationandsupportsitsclassificationasanovelspecies
AT bennettmalcolm retrievalofthecompletecodingsequenceoftheukendemictatenaleorthohantavirusrevealsextensivestrainvariationandsupportsitsclassificationasanovelspecies
AT tarlintonrachaele retrievalofthecompletecodingsequenceoftheukendemictatenaleorthohantavirusrevealsextensivestrainvariationandsupportsitsclassificationasanovelspecies
AT balljonathank retrievalofthecompletecodingsequenceoftheukendemictatenaleorthohantavirusrevealsextensivestrainvariationandsupportsitsclassificationasanovelspecies
AT mcclurecpatrick retrievalofthecompletecodingsequenceoftheukendemictatenaleorthohantavirusrevealsextensivestrainvariationandsupportsitsclassificationasanovelspecies