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Copy Number and Prevalence of Porcine Endogenous Retroviruses (PERVs) in German Wild Boars

Porcine endogenous retroviruses (PERVs) are integrated in the genome of pigs and are transmitted like cellular genes from parents to the offspring. Whereas PERV-A and PERV-B are present in all pigs, PERV-C was found to be in many, but not all pigs. When PERV-C is present, recombination with PERV-A m...

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Autores principales: Krüger, Luise, Stillfried, Milena, Prinz, Carolin, Schröder, Vanessa, Neubert, Lena Katharina, Denner, Joachim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7232352/
https://www.ncbi.nlm.nih.gov/pubmed/32276520
http://dx.doi.org/10.3390/v12040419
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author Krüger, Luise
Stillfried, Milena
Prinz, Carolin
Schröder, Vanessa
Neubert, Lena Katharina
Denner, Joachim
author_facet Krüger, Luise
Stillfried, Milena
Prinz, Carolin
Schröder, Vanessa
Neubert, Lena Katharina
Denner, Joachim
author_sort Krüger, Luise
collection PubMed
description Porcine endogenous retroviruses (PERVs) are integrated in the genome of pigs and are transmitted like cellular genes from parents to the offspring. Whereas PERV-A and PERV-B are present in all pigs, PERV-C was found to be in many, but not all pigs. When PERV-C is present, recombination with PERV-A may happen and the PERV-A/C recombinants are characterized by a high replication rate. Until now, nothing has been known about the copy number of PERVs in wild boars and little is known about the prevalence of the phylogenetically youngest PERV-C in ancient wild boars. Here we investigated for the first time the copy number of PERVs in different populations of wild boars in and around Berlin using droplet digital PCR. Copy numbers between 3 and 69 per genome have been measured. A lower number but a higher variability was found compared to domestic pigs, including minipigs reported earlier (Fiebig et al., Xenotransplantation, 2018). The wild boar populations differed genetically and had been isolated during the existence of the Berlin wall. Despite this, the variations in copy number were larger in a single population compared to the differences between the populations. PERV-C was found in all 92 analyzed animals. Differences in the copy number of PERV in different organs of a single wild boar indicate that PERVs are also active in wild boars, replicating and infecting new cells as has been shown in domestic pigs.
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spelling pubmed-72323522020-05-22 Copy Number and Prevalence of Porcine Endogenous Retroviruses (PERVs) in German Wild Boars Krüger, Luise Stillfried, Milena Prinz, Carolin Schröder, Vanessa Neubert, Lena Katharina Denner, Joachim Viruses Article Porcine endogenous retroviruses (PERVs) are integrated in the genome of pigs and are transmitted like cellular genes from parents to the offspring. Whereas PERV-A and PERV-B are present in all pigs, PERV-C was found to be in many, but not all pigs. When PERV-C is present, recombination with PERV-A may happen and the PERV-A/C recombinants are characterized by a high replication rate. Until now, nothing has been known about the copy number of PERVs in wild boars and little is known about the prevalence of the phylogenetically youngest PERV-C in ancient wild boars. Here we investigated for the first time the copy number of PERVs in different populations of wild boars in and around Berlin using droplet digital PCR. Copy numbers between 3 and 69 per genome have been measured. A lower number but a higher variability was found compared to domestic pigs, including minipigs reported earlier (Fiebig et al., Xenotransplantation, 2018). The wild boar populations differed genetically and had been isolated during the existence of the Berlin wall. Despite this, the variations in copy number were larger in a single population compared to the differences between the populations. PERV-C was found in all 92 analyzed animals. Differences in the copy number of PERV in different organs of a single wild boar indicate that PERVs are also active in wild boars, replicating and infecting new cells as has been shown in domestic pigs. MDPI 2020-04-08 /pmc/articles/PMC7232352/ /pubmed/32276520 http://dx.doi.org/10.3390/v12040419 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Krüger, Luise
Stillfried, Milena
Prinz, Carolin
Schröder, Vanessa
Neubert, Lena Katharina
Denner, Joachim
Copy Number and Prevalence of Porcine Endogenous Retroviruses (PERVs) in German Wild Boars
title Copy Number and Prevalence of Porcine Endogenous Retroviruses (PERVs) in German Wild Boars
title_full Copy Number and Prevalence of Porcine Endogenous Retroviruses (PERVs) in German Wild Boars
title_fullStr Copy Number and Prevalence of Porcine Endogenous Retroviruses (PERVs) in German Wild Boars
title_full_unstemmed Copy Number and Prevalence of Porcine Endogenous Retroviruses (PERVs) in German Wild Boars
title_short Copy Number and Prevalence of Porcine Endogenous Retroviruses (PERVs) in German Wild Boars
title_sort copy number and prevalence of porcine endogenous retroviruses (pervs) in german wild boars
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7232352/
https://www.ncbi.nlm.nih.gov/pubmed/32276520
http://dx.doi.org/10.3390/v12040419
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