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Incidental Prophylactic Appendectomy Is Associated with a Profound Microbial Dysbiosis in the Long-Term

Incidental prophylactic surgeries are performed in certain situations. Incidental prophylactic appendectomies were common practice within opened bariatric surgeries. The gut microbiota has emerged as an important actor within the homeostasis of the host. A new hypothesis has been formulated about th...

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Autores principales: Sánchez-Alcoholado, Lidia, Fernández-García, José Carlos, Gutiérrez-Repiso, Carolina, Bernal-López, M Rosa, Ocaña-Wilhelmi, Luis, García-Fuentes, Eduardo, Moreno-Indias, Isabel, Tinahones, Francisco J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7232405/
https://www.ncbi.nlm.nih.gov/pubmed/32340272
http://dx.doi.org/10.3390/microorganisms8040609
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author Sánchez-Alcoholado, Lidia
Fernández-García, José Carlos
Gutiérrez-Repiso, Carolina
Bernal-López, M Rosa
Ocaña-Wilhelmi, Luis
García-Fuentes, Eduardo
Moreno-Indias, Isabel
Tinahones, Francisco J.
author_facet Sánchez-Alcoholado, Lidia
Fernández-García, José Carlos
Gutiérrez-Repiso, Carolina
Bernal-López, M Rosa
Ocaña-Wilhelmi, Luis
García-Fuentes, Eduardo
Moreno-Indias, Isabel
Tinahones, Francisco J.
author_sort Sánchez-Alcoholado, Lidia
collection PubMed
description Incidental prophylactic surgeries are performed in certain situations. Incidental prophylactic appendectomies were common practice within opened bariatric surgeries. The gut microbiota has emerged as an important actor within the homeostasis of the host. A new hypothesis has been formulated about the appendix function in relation to gut microbiota. Our objective was to study the gut microbiota profiles of patients that had suffered from an incidental prophylactic appendectomy during their bariatric surgeries, while comparing them to patients whose appendixes had remained intact. A case-control observational prospective study of 40 patients who underwent bariatric surgery, with or without an incidental prophylactic appendectomy, during 2004–2008 with an evaluation of their gut microbiota populations at the end of 2016 was conducted by sequencing the 16 S rRNA gene by Next Generation Sequencing of patients’ stools and appendix tissues. Patients with their appendix removed showed lower levels of richness and diversity of their gut microbiota populations. Odoribacter, Bilophila, Butyricimonas, and Faecalibacterium levels were increased in the Intact group, while Lachnobacterium suffered an expansion in the group without the appendix. Moreover, a linear regression model introduced the concept that Butyricimonas and Odoribacter may be implicated in insulin regulation. Thus, gut microbiota should be considered in the decisions of practical surgery, regarding the appendix as a mediator of homeostasis in the host. Butyricimonas and Odoribacter require further investigation as key bacteria implicated in insulin regulation.
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spelling pubmed-72324052020-05-22 Incidental Prophylactic Appendectomy Is Associated with a Profound Microbial Dysbiosis in the Long-Term Sánchez-Alcoholado, Lidia Fernández-García, José Carlos Gutiérrez-Repiso, Carolina Bernal-López, M Rosa Ocaña-Wilhelmi, Luis García-Fuentes, Eduardo Moreno-Indias, Isabel Tinahones, Francisco J. Microorganisms Article Incidental prophylactic surgeries are performed in certain situations. Incidental prophylactic appendectomies were common practice within opened bariatric surgeries. The gut microbiota has emerged as an important actor within the homeostasis of the host. A new hypothesis has been formulated about the appendix function in relation to gut microbiota. Our objective was to study the gut microbiota profiles of patients that had suffered from an incidental prophylactic appendectomy during their bariatric surgeries, while comparing them to patients whose appendixes had remained intact. A case-control observational prospective study of 40 patients who underwent bariatric surgery, with or without an incidental prophylactic appendectomy, during 2004–2008 with an evaluation of their gut microbiota populations at the end of 2016 was conducted by sequencing the 16 S rRNA gene by Next Generation Sequencing of patients’ stools and appendix tissues. Patients with their appendix removed showed lower levels of richness and diversity of their gut microbiota populations. Odoribacter, Bilophila, Butyricimonas, and Faecalibacterium levels were increased in the Intact group, while Lachnobacterium suffered an expansion in the group without the appendix. Moreover, a linear regression model introduced the concept that Butyricimonas and Odoribacter may be implicated in insulin regulation. Thus, gut microbiota should be considered in the decisions of practical surgery, regarding the appendix as a mediator of homeostasis in the host. Butyricimonas and Odoribacter require further investigation as key bacteria implicated in insulin regulation. MDPI 2020-04-23 /pmc/articles/PMC7232405/ /pubmed/32340272 http://dx.doi.org/10.3390/microorganisms8040609 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sánchez-Alcoholado, Lidia
Fernández-García, José Carlos
Gutiérrez-Repiso, Carolina
Bernal-López, M Rosa
Ocaña-Wilhelmi, Luis
García-Fuentes, Eduardo
Moreno-Indias, Isabel
Tinahones, Francisco J.
Incidental Prophylactic Appendectomy Is Associated with a Profound Microbial Dysbiosis in the Long-Term
title Incidental Prophylactic Appendectomy Is Associated with a Profound Microbial Dysbiosis in the Long-Term
title_full Incidental Prophylactic Appendectomy Is Associated with a Profound Microbial Dysbiosis in the Long-Term
title_fullStr Incidental Prophylactic Appendectomy Is Associated with a Profound Microbial Dysbiosis in the Long-Term
title_full_unstemmed Incidental Prophylactic Appendectomy Is Associated with a Profound Microbial Dysbiosis in the Long-Term
title_short Incidental Prophylactic Appendectomy Is Associated with a Profound Microbial Dysbiosis in the Long-Term
title_sort incidental prophylactic appendectomy is associated with a profound microbial dysbiosis in the long-term
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7232405/
https://www.ncbi.nlm.nih.gov/pubmed/32340272
http://dx.doi.org/10.3390/microorganisms8040609
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