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Candida albicans Adaptation on Simulated Human Body Fluids under Different pH

Candida albicans remains the most problematic of all Candida species, causing severe infections. Adaptation to different human body niches, such oral and urinary tracts, has been shown to be essential for survival and critical for virulence of C. albicans. Thus, the present work aimed to study the b...

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Autores principales: Barbosa, Ana, Araújo, Daniela, Ribeiro, Eduarda, Henriques, Mariana, Silva, Sónia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7232421/
https://www.ncbi.nlm.nih.gov/pubmed/32260085
http://dx.doi.org/10.3390/microorganisms8040511
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author Barbosa, Ana
Araújo, Daniela
Ribeiro, Eduarda
Henriques, Mariana
Silva, Sónia
author_facet Barbosa, Ana
Araújo, Daniela
Ribeiro, Eduarda
Henriques, Mariana
Silva, Sónia
author_sort Barbosa, Ana
collection PubMed
description Candida albicans remains the most problematic of all Candida species, causing severe infections. Adaptation to different human body niches, such oral and urinary tracts, has been shown to be essential for survival and critical for virulence of C. albicans. Thus, the present work aimed to study the behaviour of C. albicans on simulated human body fluids (artificial saliva and urine) at different values of pH (pH 5.8 and 7) by determining its ability to develop two of the most important virulence factors: biofilms and filamentous forms. Under this study, it was demonstrated that C. albicans was able to grow as free cells and to develop biofilm communities composed of multiple cell types (yeast and elongated hyphal cells) on both simulated human body fluids and under different pH. It was interesting to note that the pH had little impact on C. albicans planktonic and biofilm growth, despite influencing the development of filamentous shapes in artificial saliva and urine. So, it was possible to infer that C. albicans presents a high plasticity and adaptability to different human body fluids, namely saliva and urine. These can be the justification for the high number of oral and urinary candidiasis in the whole world.
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spelling pubmed-72324212020-05-22 Candida albicans Adaptation on Simulated Human Body Fluids under Different pH Barbosa, Ana Araújo, Daniela Ribeiro, Eduarda Henriques, Mariana Silva, Sónia Microorganisms Article Candida albicans remains the most problematic of all Candida species, causing severe infections. Adaptation to different human body niches, such oral and urinary tracts, has been shown to be essential for survival and critical for virulence of C. albicans. Thus, the present work aimed to study the behaviour of C. albicans on simulated human body fluids (artificial saliva and urine) at different values of pH (pH 5.8 and 7) by determining its ability to develop two of the most important virulence factors: biofilms and filamentous forms. Under this study, it was demonstrated that C. albicans was able to grow as free cells and to develop biofilm communities composed of multiple cell types (yeast and elongated hyphal cells) on both simulated human body fluids and under different pH. It was interesting to note that the pH had little impact on C. albicans planktonic and biofilm growth, despite influencing the development of filamentous shapes in artificial saliva and urine. So, it was possible to infer that C. albicans presents a high plasticity and adaptability to different human body fluids, namely saliva and urine. These can be the justification for the high number of oral and urinary candidiasis in the whole world. MDPI 2020-04-03 /pmc/articles/PMC7232421/ /pubmed/32260085 http://dx.doi.org/10.3390/microorganisms8040511 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Barbosa, Ana
Araújo, Daniela
Ribeiro, Eduarda
Henriques, Mariana
Silva, Sónia
Candida albicans Adaptation on Simulated Human Body Fluids under Different pH
title Candida albicans Adaptation on Simulated Human Body Fluids under Different pH
title_full Candida albicans Adaptation on Simulated Human Body Fluids under Different pH
title_fullStr Candida albicans Adaptation on Simulated Human Body Fluids under Different pH
title_full_unstemmed Candida albicans Adaptation on Simulated Human Body Fluids under Different pH
title_short Candida albicans Adaptation on Simulated Human Body Fluids under Different pH
title_sort candida albicans adaptation on simulated human body fluids under different ph
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7232421/
https://www.ncbi.nlm.nih.gov/pubmed/32260085
http://dx.doi.org/10.3390/microorganisms8040511
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