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Suppression of nbe-miR1919c-5p Expression in Nicotiana benthamiana Enhances Tobacco Curly Shoot Virus and Its Betasatellite Co-Infection

MicroRNAs (miRNAs) are non-coding but functional RNA molecules of 21–25 nucleotides in length. MiRNAs play significant regulatory roles in diverse plant biological processes. In order to decipher the relationship between nbe-miR1919c-5p and the accumulations of tobacco curly shoot virus (TbCSV) and...

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Autores principales: Du, Jiang, Wu, Rui, Liu, Zhuoying, Sun, Miao, Ghanem, Hussein, Li, Mingjun, Wu, Gentu, Qing, Ling
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7232422/
https://www.ncbi.nlm.nih.gov/pubmed/32244650
http://dx.doi.org/10.3390/v12040392
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author Du, Jiang
Wu, Rui
Liu, Zhuoying
Sun, Miao
Ghanem, Hussein
Li, Mingjun
Wu, Gentu
Qing, Ling
author_facet Du, Jiang
Wu, Rui
Liu, Zhuoying
Sun, Miao
Ghanem, Hussein
Li, Mingjun
Wu, Gentu
Qing, Ling
author_sort Du, Jiang
collection PubMed
description MicroRNAs (miRNAs) are non-coding but functional RNA molecules of 21–25 nucleotides in length. MiRNAs play significant regulatory roles in diverse plant biological processes. In order to decipher the relationship between nbe-miR1919c-5p and the accumulations of tobacco curly shoot virus (TbCSV) and its betasatellite (TbCSB) DNAs, as well as viral symptom development, we investigated the function of nbe-miR1919c-5p during TbCSV and TbCSB co-infection in plants using a PVX-and a TRV-based short tandem target mimic (STTM) technology. Suppression of nbe-miR1919c-5p expression using these two technologies enhanced TbCSV and TbCSB co-infection-induced leaf curling symptoms in Nicotiana benthamiana plants. Furthermore, suppression of nbe-miR1919c-5p expression enhanced TbCSV and TbCSB DNA accumulations in the infected plants. Our results can advance our knowledge on the nbe-miR1919c-5p function during TbCSV and TbCSB co-infection.
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spelling pubmed-72324222020-05-22 Suppression of nbe-miR1919c-5p Expression in Nicotiana benthamiana Enhances Tobacco Curly Shoot Virus and Its Betasatellite Co-Infection Du, Jiang Wu, Rui Liu, Zhuoying Sun, Miao Ghanem, Hussein Li, Mingjun Wu, Gentu Qing, Ling Viruses Article MicroRNAs (miRNAs) are non-coding but functional RNA molecules of 21–25 nucleotides in length. MiRNAs play significant regulatory roles in diverse plant biological processes. In order to decipher the relationship between nbe-miR1919c-5p and the accumulations of tobacco curly shoot virus (TbCSV) and its betasatellite (TbCSB) DNAs, as well as viral symptom development, we investigated the function of nbe-miR1919c-5p during TbCSV and TbCSB co-infection in plants using a PVX-and a TRV-based short tandem target mimic (STTM) technology. Suppression of nbe-miR1919c-5p expression using these two technologies enhanced TbCSV and TbCSB co-infection-induced leaf curling symptoms in Nicotiana benthamiana plants. Furthermore, suppression of nbe-miR1919c-5p expression enhanced TbCSV and TbCSB DNA accumulations in the infected plants. Our results can advance our knowledge on the nbe-miR1919c-5p function during TbCSV and TbCSB co-infection. MDPI 2020-04-01 /pmc/articles/PMC7232422/ /pubmed/32244650 http://dx.doi.org/10.3390/v12040392 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Du, Jiang
Wu, Rui
Liu, Zhuoying
Sun, Miao
Ghanem, Hussein
Li, Mingjun
Wu, Gentu
Qing, Ling
Suppression of nbe-miR1919c-5p Expression in Nicotiana benthamiana Enhances Tobacco Curly Shoot Virus and Its Betasatellite Co-Infection
title Suppression of nbe-miR1919c-5p Expression in Nicotiana benthamiana Enhances Tobacco Curly Shoot Virus and Its Betasatellite Co-Infection
title_full Suppression of nbe-miR1919c-5p Expression in Nicotiana benthamiana Enhances Tobacco Curly Shoot Virus and Its Betasatellite Co-Infection
title_fullStr Suppression of nbe-miR1919c-5p Expression in Nicotiana benthamiana Enhances Tobacco Curly Shoot Virus and Its Betasatellite Co-Infection
title_full_unstemmed Suppression of nbe-miR1919c-5p Expression in Nicotiana benthamiana Enhances Tobacco Curly Shoot Virus and Its Betasatellite Co-Infection
title_short Suppression of nbe-miR1919c-5p Expression in Nicotiana benthamiana Enhances Tobacco Curly Shoot Virus and Its Betasatellite Co-Infection
title_sort suppression of nbe-mir1919c-5p expression in nicotiana benthamiana enhances tobacco curly shoot virus and its betasatellite co-infection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7232422/
https://www.ncbi.nlm.nih.gov/pubmed/32244650
http://dx.doi.org/10.3390/v12040392
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