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Orthohantavirus Isolated in Reservoir Host Cells Displays Minimal Genetic Changes and Retains Wild-Type Infection Properties
Orthohantaviruses are globally emerging zoonotic pathogens. While the reservoir host role of several rodent species is well-established, detailed research on the mechanisms of host-othohantavirus interactions has been constrained by the lack of an experimental system that is able to effectively repl...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7232471/ https://www.ncbi.nlm.nih.gov/pubmed/32316667 http://dx.doi.org/10.3390/v12040457 |
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author | Strandin, Tomas Smura, Teemu Ahola, Paula Aaltonen, Kirsi Sironen, Tarja Hepojoki, Jussi Eckerle, Isabella Ulrich, Rainer G. Vapalahti, Olli Kipar, Anja Forbes, Kristian M. |
author_facet | Strandin, Tomas Smura, Teemu Ahola, Paula Aaltonen, Kirsi Sironen, Tarja Hepojoki, Jussi Eckerle, Isabella Ulrich, Rainer G. Vapalahti, Olli Kipar, Anja Forbes, Kristian M. |
author_sort | Strandin, Tomas |
collection | PubMed |
description | Orthohantaviruses are globally emerging zoonotic pathogens. While the reservoir host role of several rodent species is well-established, detailed research on the mechanisms of host-othohantavirus interactions has been constrained by the lack of an experimental system that is able to effectively replicate natural infections in controlled settings. Here we report the isolation, and genetic and phenotypic characterization of a novel Puumala orthohantavirus (PUUV) in cells derived from its reservoir host, the bank vole. The isolation process resulted in cell culture infection that evaded antiviral responses, persisted cell passaging, and had minor viral genome alterations. Critically, experimental infections of bank voles with the new isolate resembled natural infections in terms of viral load and host cell distribution. When compared to an attenuated Vero E6 cell-adapted PUUV Kazan strain, the novel isolate demonstrated delayed virus-specific humoral responses. A lack of virus-specific antibodies was also observed during experimental infections with wild-type PUUV, suggesting that delayed seroconversion could be a general phenomenon during orthohantavirus infection in reservoir hosts. Our results demonstrate that orthohantavirus isolation on cells derived from a vole reservoir host retains wild-type infection properties and should be considered the method of choice for experimental infection models to replicate natural processes. |
format | Online Article Text |
id | pubmed-7232471 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-72324712020-05-22 Orthohantavirus Isolated in Reservoir Host Cells Displays Minimal Genetic Changes and Retains Wild-Type Infection Properties Strandin, Tomas Smura, Teemu Ahola, Paula Aaltonen, Kirsi Sironen, Tarja Hepojoki, Jussi Eckerle, Isabella Ulrich, Rainer G. Vapalahti, Olli Kipar, Anja Forbes, Kristian M. Viruses Article Orthohantaviruses are globally emerging zoonotic pathogens. While the reservoir host role of several rodent species is well-established, detailed research on the mechanisms of host-othohantavirus interactions has been constrained by the lack of an experimental system that is able to effectively replicate natural infections in controlled settings. Here we report the isolation, and genetic and phenotypic characterization of a novel Puumala orthohantavirus (PUUV) in cells derived from its reservoir host, the bank vole. The isolation process resulted in cell culture infection that evaded antiviral responses, persisted cell passaging, and had minor viral genome alterations. Critically, experimental infections of bank voles with the new isolate resembled natural infections in terms of viral load and host cell distribution. When compared to an attenuated Vero E6 cell-adapted PUUV Kazan strain, the novel isolate demonstrated delayed virus-specific humoral responses. A lack of virus-specific antibodies was also observed during experimental infections with wild-type PUUV, suggesting that delayed seroconversion could be a general phenomenon during orthohantavirus infection in reservoir hosts. Our results demonstrate that orthohantavirus isolation on cells derived from a vole reservoir host retains wild-type infection properties and should be considered the method of choice for experimental infection models to replicate natural processes. MDPI 2020-04-17 /pmc/articles/PMC7232471/ /pubmed/32316667 http://dx.doi.org/10.3390/v12040457 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Strandin, Tomas Smura, Teemu Ahola, Paula Aaltonen, Kirsi Sironen, Tarja Hepojoki, Jussi Eckerle, Isabella Ulrich, Rainer G. Vapalahti, Olli Kipar, Anja Forbes, Kristian M. Orthohantavirus Isolated in Reservoir Host Cells Displays Minimal Genetic Changes and Retains Wild-Type Infection Properties |
title | Orthohantavirus Isolated in Reservoir Host Cells Displays Minimal Genetic Changes and Retains Wild-Type Infection Properties |
title_full | Orthohantavirus Isolated in Reservoir Host Cells Displays Minimal Genetic Changes and Retains Wild-Type Infection Properties |
title_fullStr | Orthohantavirus Isolated in Reservoir Host Cells Displays Minimal Genetic Changes and Retains Wild-Type Infection Properties |
title_full_unstemmed | Orthohantavirus Isolated in Reservoir Host Cells Displays Minimal Genetic Changes and Retains Wild-Type Infection Properties |
title_short | Orthohantavirus Isolated in Reservoir Host Cells Displays Minimal Genetic Changes and Retains Wild-Type Infection Properties |
title_sort | orthohantavirus isolated in reservoir host cells displays minimal genetic changes and retains wild-type infection properties |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7232471/ https://www.ncbi.nlm.nih.gov/pubmed/32316667 http://dx.doi.org/10.3390/v12040457 |
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