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Novel Toscana Virus Reverse Genetics System Establishes NSs as an Antagonist of Type I Interferon Responses
The sand fly-borne Toscana virus (TOSV) is the major cause of human meningoencephalitis in the Mediterranean basin during the summer season. In this work, we have developed a T7 RNA polymerase-driven reverse genetics system to recover infectious particles of a lineage B strain of TOSV. The viral pro...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7232479/ https://www.ncbi.nlm.nih.gov/pubmed/32260371 http://dx.doi.org/10.3390/v12040400 |
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author | Woelfl, Franziska Léger, Psylvia Oreshkova, Nadia Pahmeier, Felix Windhaber, Stefan Koch, Jana Stanifer, Megan Roman Sosa, Gleyder Uckeley, Zina M. Rey, Felix A. Boulant, Steeve Kortekaas, Jeroen Wichgers Schreur, Paul J. Lozach, Pierre-Yves |
author_facet | Woelfl, Franziska Léger, Psylvia Oreshkova, Nadia Pahmeier, Felix Windhaber, Stefan Koch, Jana Stanifer, Megan Roman Sosa, Gleyder Uckeley, Zina M. Rey, Felix A. Boulant, Steeve Kortekaas, Jeroen Wichgers Schreur, Paul J. Lozach, Pierre-Yves |
author_sort | Woelfl, Franziska |
collection | PubMed |
description | The sand fly-borne Toscana virus (TOSV) is the major cause of human meningoencephalitis in the Mediterranean basin during the summer season. In this work, we have developed a T7 RNA polymerase-driven reverse genetics system to recover infectious particles of a lineage B strain of TOSV. The viral protein pattern and growth properties of the rescued virus (rTOSV) were found to be similar to those of the corresponding wild-type (wt) virus. Using this system, we genetically engineered a TOSV mutant lacking expression of the non-structural protein NSs (rTOSVɸNSs). Unlike rTOSV and the wt virus, rTOSVɸNSs was unable to (i) suppress interferon (IFN)-b messenger RNA induction; and (ii) grow efficiently in cells producing IFN-b. Together, our results highlight the importance of NSs for TOSV in evading the IFN response and provide a comprehensive toolbox to investigate the TOSV life cycle in mammalian and insect host cells, including several novel polyclonal antibodies. |
format | Online Article Text |
id | pubmed-7232479 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-72324792020-05-22 Novel Toscana Virus Reverse Genetics System Establishes NSs as an Antagonist of Type I Interferon Responses Woelfl, Franziska Léger, Psylvia Oreshkova, Nadia Pahmeier, Felix Windhaber, Stefan Koch, Jana Stanifer, Megan Roman Sosa, Gleyder Uckeley, Zina M. Rey, Felix A. Boulant, Steeve Kortekaas, Jeroen Wichgers Schreur, Paul J. Lozach, Pierre-Yves Viruses Article The sand fly-borne Toscana virus (TOSV) is the major cause of human meningoencephalitis in the Mediterranean basin during the summer season. In this work, we have developed a T7 RNA polymerase-driven reverse genetics system to recover infectious particles of a lineage B strain of TOSV. The viral protein pattern and growth properties of the rescued virus (rTOSV) were found to be similar to those of the corresponding wild-type (wt) virus. Using this system, we genetically engineered a TOSV mutant lacking expression of the non-structural protein NSs (rTOSVɸNSs). Unlike rTOSV and the wt virus, rTOSVɸNSs was unable to (i) suppress interferon (IFN)-b messenger RNA induction; and (ii) grow efficiently in cells producing IFN-b. Together, our results highlight the importance of NSs for TOSV in evading the IFN response and provide a comprehensive toolbox to investigate the TOSV life cycle in mammalian and insect host cells, including several novel polyclonal antibodies. MDPI 2020-04-04 /pmc/articles/PMC7232479/ /pubmed/32260371 http://dx.doi.org/10.3390/v12040400 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Woelfl, Franziska Léger, Psylvia Oreshkova, Nadia Pahmeier, Felix Windhaber, Stefan Koch, Jana Stanifer, Megan Roman Sosa, Gleyder Uckeley, Zina M. Rey, Felix A. Boulant, Steeve Kortekaas, Jeroen Wichgers Schreur, Paul J. Lozach, Pierre-Yves Novel Toscana Virus Reverse Genetics System Establishes NSs as an Antagonist of Type I Interferon Responses |
title | Novel Toscana Virus Reverse Genetics System Establishes NSs as an Antagonist of Type I Interferon Responses |
title_full | Novel Toscana Virus Reverse Genetics System Establishes NSs as an Antagonist of Type I Interferon Responses |
title_fullStr | Novel Toscana Virus Reverse Genetics System Establishes NSs as an Antagonist of Type I Interferon Responses |
title_full_unstemmed | Novel Toscana Virus Reverse Genetics System Establishes NSs as an Antagonist of Type I Interferon Responses |
title_short | Novel Toscana Virus Reverse Genetics System Establishes NSs as an Antagonist of Type I Interferon Responses |
title_sort | novel toscana virus reverse genetics system establishes nss as an antagonist of type i interferon responses |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7232479/ https://www.ncbi.nlm.nih.gov/pubmed/32260371 http://dx.doi.org/10.3390/v12040400 |
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