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Identification and RNAi Profile of a Novel Iflavirus Infecting Senegalese Aedes vexans arabiensis Mosquitoes

The inland floodwater mosquito Aedes vexans (Meigen, 1830) is a competent vector of numerous arthropod-borne viruses such as Rift Valley fever virus (Phenuiviridae) and Zika virus (Flaviviridae). Aedes vexans spp. have widespread Afrotropical distribution and are common European cosmopolitan mosquit...

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Autores principales: Parry, Rhys, Naccache, Fanny, Ndiaye, El Hadji, Fall, Gamou, Castelli, Ilaria, Lühken, Renke, Medlock, Jolyon, Cull, Benjamin, Hesson, Jenny C., Montarsi, Fabrizio, Failloux, Anna-Bella, Kohl, Alain, Schnettler, Esther, Diallo, Mawlouth, Asgari, Sassan, Dietrich, Isabelle, Becker, Stefanie C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7232509/
https://www.ncbi.nlm.nih.gov/pubmed/32295109
http://dx.doi.org/10.3390/v12040440
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author Parry, Rhys
Naccache, Fanny
Ndiaye, El Hadji
Fall, Gamou
Castelli, Ilaria
Lühken, Renke
Medlock, Jolyon
Cull, Benjamin
Hesson, Jenny C.
Montarsi, Fabrizio
Failloux, Anna-Bella
Kohl, Alain
Schnettler, Esther
Diallo, Mawlouth
Asgari, Sassan
Dietrich, Isabelle
Becker, Stefanie C.
author_facet Parry, Rhys
Naccache, Fanny
Ndiaye, El Hadji
Fall, Gamou
Castelli, Ilaria
Lühken, Renke
Medlock, Jolyon
Cull, Benjamin
Hesson, Jenny C.
Montarsi, Fabrizio
Failloux, Anna-Bella
Kohl, Alain
Schnettler, Esther
Diallo, Mawlouth
Asgari, Sassan
Dietrich, Isabelle
Becker, Stefanie C.
author_sort Parry, Rhys
collection PubMed
description The inland floodwater mosquito Aedes vexans (Meigen, 1830) is a competent vector of numerous arthropod-borne viruses such as Rift Valley fever virus (Phenuiviridae) and Zika virus (Flaviviridae). Aedes vexans spp. have widespread Afrotropical distribution and are common European cosmopolitan mosquitoes. We examined the virome of Ae. vexans arabiensis samples from Barkédji village, Senegal, with small RNA sequencing, bioinformatic analysis, and RT-PCR screening. We identified a novel 9494 nt iflavirus (Picornaviridae) designated here as Aedes vexans iflavirus (AvIFV). Annotation of the AvIFV genome reveals a 2782 amino acid polyprotein with iflavirus protein domain architecture and typical iflavirus 5’ internal ribosomal entry site and 3’ poly-A tail. Aedes vexans iflavirus is most closely related to a partial virus sequence from Venturia canescens (a parasitoid wasp) with 56.77% pairwise amino acid identity. Analysis of AvIFV-derived small RNAs suggests that AvIFV is targeted by the exogenous RNA interference pathway but not the PIWI-interacting RNA response, as ~60% of AvIFV reads corresponded to 21 nt Dicer-2 virus-derived small RNAs and the 24–29 nt AvIFV read population did not exhibit a “ping-pong” signature. The RT-PCR screens of archival and current (circa 2011–2020) Ae. vexans arabiensis laboratory samples and wild-caught mosquitoes from Barkédji suggest that AvIFV is ubiquitous in these mosquitoes. Further, we screened wild-caught European Ae. vexans samples from Germany, the United Kingdom, Italy, and Sweden, all of which tested negative for AvIFV RNA. This report provides insight into the diversity of commensal Aedes viruses and the host RNAi response towards iflaviruses.
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spelling pubmed-72325092020-05-22 Identification and RNAi Profile of a Novel Iflavirus Infecting Senegalese Aedes vexans arabiensis Mosquitoes Parry, Rhys Naccache, Fanny Ndiaye, El Hadji Fall, Gamou Castelli, Ilaria Lühken, Renke Medlock, Jolyon Cull, Benjamin Hesson, Jenny C. Montarsi, Fabrizio Failloux, Anna-Bella Kohl, Alain Schnettler, Esther Diallo, Mawlouth Asgari, Sassan Dietrich, Isabelle Becker, Stefanie C. Viruses Article The inland floodwater mosquito Aedes vexans (Meigen, 1830) is a competent vector of numerous arthropod-borne viruses such as Rift Valley fever virus (Phenuiviridae) and Zika virus (Flaviviridae). Aedes vexans spp. have widespread Afrotropical distribution and are common European cosmopolitan mosquitoes. We examined the virome of Ae. vexans arabiensis samples from Barkédji village, Senegal, with small RNA sequencing, bioinformatic analysis, and RT-PCR screening. We identified a novel 9494 nt iflavirus (Picornaviridae) designated here as Aedes vexans iflavirus (AvIFV). Annotation of the AvIFV genome reveals a 2782 amino acid polyprotein with iflavirus protein domain architecture and typical iflavirus 5’ internal ribosomal entry site and 3’ poly-A tail. Aedes vexans iflavirus is most closely related to a partial virus sequence from Venturia canescens (a parasitoid wasp) with 56.77% pairwise amino acid identity. Analysis of AvIFV-derived small RNAs suggests that AvIFV is targeted by the exogenous RNA interference pathway but not the PIWI-interacting RNA response, as ~60% of AvIFV reads corresponded to 21 nt Dicer-2 virus-derived small RNAs and the 24–29 nt AvIFV read population did not exhibit a “ping-pong” signature. The RT-PCR screens of archival and current (circa 2011–2020) Ae. vexans arabiensis laboratory samples and wild-caught mosquitoes from Barkédji suggest that AvIFV is ubiquitous in these mosquitoes. Further, we screened wild-caught European Ae. vexans samples from Germany, the United Kingdom, Italy, and Sweden, all of which tested negative for AvIFV RNA. This report provides insight into the diversity of commensal Aedes viruses and the host RNAi response towards iflaviruses. MDPI 2020-04-14 /pmc/articles/PMC7232509/ /pubmed/32295109 http://dx.doi.org/10.3390/v12040440 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Parry, Rhys
Naccache, Fanny
Ndiaye, El Hadji
Fall, Gamou
Castelli, Ilaria
Lühken, Renke
Medlock, Jolyon
Cull, Benjamin
Hesson, Jenny C.
Montarsi, Fabrizio
Failloux, Anna-Bella
Kohl, Alain
Schnettler, Esther
Diallo, Mawlouth
Asgari, Sassan
Dietrich, Isabelle
Becker, Stefanie C.
Identification and RNAi Profile of a Novel Iflavirus Infecting Senegalese Aedes vexans arabiensis Mosquitoes
title Identification and RNAi Profile of a Novel Iflavirus Infecting Senegalese Aedes vexans arabiensis Mosquitoes
title_full Identification and RNAi Profile of a Novel Iflavirus Infecting Senegalese Aedes vexans arabiensis Mosquitoes
title_fullStr Identification and RNAi Profile of a Novel Iflavirus Infecting Senegalese Aedes vexans arabiensis Mosquitoes
title_full_unstemmed Identification and RNAi Profile of a Novel Iflavirus Infecting Senegalese Aedes vexans arabiensis Mosquitoes
title_short Identification and RNAi Profile of a Novel Iflavirus Infecting Senegalese Aedes vexans arabiensis Mosquitoes
title_sort identification and rnai profile of a novel iflavirus infecting senegalese aedes vexans arabiensis mosquitoes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7232509/
https://www.ncbi.nlm.nih.gov/pubmed/32295109
http://dx.doi.org/10.3390/v12040440
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