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The Tomato spotted wilt virus (TSWV) Genome is Differentially Targeted in TSWV-Infected Tomato (Solanum lycopersicum) with or without Sw-5 Gene

Tospoviruses cause significant losses to a wide range of agronomic and horticultural crops worldwide. The type member, Tomato spotted wilt tospovirus (TSWV), causes systemic infection in susceptible tomato cultivars, whereas its infection is localized in cultivars carrying the Sw-5 resistance gene....

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Autores principales: Olaya, Cristian, Fletcher, Stephen J., Zhai, Ying, Peters, Jonathan, Margaria, Paolo, Winter, Stephan, Mitter, Neena, Pappu, Hanu R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7232525/
https://www.ncbi.nlm.nih.gov/pubmed/32224858
http://dx.doi.org/10.3390/v12040363
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author Olaya, Cristian
Fletcher, Stephen J.
Zhai, Ying
Peters, Jonathan
Margaria, Paolo
Winter, Stephan
Mitter, Neena
Pappu, Hanu R.
author_facet Olaya, Cristian
Fletcher, Stephen J.
Zhai, Ying
Peters, Jonathan
Margaria, Paolo
Winter, Stephan
Mitter, Neena
Pappu, Hanu R.
author_sort Olaya, Cristian
collection PubMed
description Tospoviruses cause significant losses to a wide range of agronomic and horticultural crops worldwide. The type member, Tomato spotted wilt tospovirus (TSWV), causes systemic infection in susceptible tomato cultivars, whereas its infection is localized in cultivars carrying the Sw-5 resistance gene. The response to TSWV infection in tomato cultivars with or without Sw-5 was determined at the virus small RNA level in the locally infected leaf. Predicted reads were aligned to TSWV reference sequences. The TSWV genome was found to be differentially processed among each of the three-viral genomic RNAs—Large (L), Medium (M) and Small (S)—in the Sw-5(+) compared to Sw-5(−) genotypes. In the Sw-5(+) cultivar, the L RNA had the highest number of viral small-interfering RNAs (vsiRNAs), whereas in the Sw-5(−) cultivar the number was higher in the S RNA. Among the three-viral genomic RNAs, the distribution of hotspots showed a higher number of reads per million reads of vsiRNAs of 21 and 22 nt class at the 5′ and 3′ ends of the L and the S RNAs, with less coverage in the M RNA. In the Sw-5(−) cultivar, the nature of the 5′ nucleotide-end in the siRNAs varied significantly; reads with 5′-adenine-end were most abundant in the mock control, whereas cytosine and uracil were more abundant in the infected plants. No such differences were seen in case of the resistant genotype. Findings provided insights into the response of tomato cultivars to TSWV infection.
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spelling pubmed-72325252020-05-22 The Tomato spotted wilt virus (TSWV) Genome is Differentially Targeted in TSWV-Infected Tomato (Solanum lycopersicum) with or without Sw-5 Gene Olaya, Cristian Fletcher, Stephen J. Zhai, Ying Peters, Jonathan Margaria, Paolo Winter, Stephan Mitter, Neena Pappu, Hanu R. Viruses Article Tospoviruses cause significant losses to a wide range of agronomic and horticultural crops worldwide. The type member, Tomato spotted wilt tospovirus (TSWV), causes systemic infection in susceptible tomato cultivars, whereas its infection is localized in cultivars carrying the Sw-5 resistance gene. The response to TSWV infection in tomato cultivars with or without Sw-5 was determined at the virus small RNA level in the locally infected leaf. Predicted reads were aligned to TSWV reference sequences. The TSWV genome was found to be differentially processed among each of the three-viral genomic RNAs—Large (L), Medium (M) and Small (S)—in the Sw-5(+) compared to Sw-5(−) genotypes. In the Sw-5(+) cultivar, the L RNA had the highest number of viral small-interfering RNAs (vsiRNAs), whereas in the Sw-5(−) cultivar the number was higher in the S RNA. Among the three-viral genomic RNAs, the distribution of hotspots showed a higher number of reads per million reads of vsiRNAs of 21 and 22 nt class at the 5′ and 3′ ends of the L and the S RNAs, with less coverage in the M RNA. In the Sw-5(−) cultivar, the nature of the 5′ nucleotide-end in the siRNAs varied significantly; reads with 5′-adenine-end were most abundant in the mock control, whereas cytosine and uracil were more abundant in the infected plants. No such differences were seen in case of the resistant genotype. Findings provided insights into the response of tomato cultivars to TSWV infection. MDPI 2020-03-26 /pmc/articles/PMC7232525/ /pubmed/32224858 http://dx.doi.org/10.3390/v12040363 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Olaya, Cristian
Fletcher, Stephen J.
Zhai, Ying
Peters, Jonathan
Margaria, Paolo
Winter, Stephan
Mitter, Neena
Pappu, Hanu R.
The Tomato spotted wilt virus (TSWV) Genome is Differentially Targeted in TSWV-Infected Tomato (Solanum lycopersicum) with or without Sw-5 Gene
title The Tomato spotted wilt virus (TSWV) Genome is Differentially Targeted in TSWV-Infected Tomato (Solanum lycopersicum) with or without Sw-5 Gene
title_full The Tomato spotted wilt virus (TSWV) Genome is Differentially Targeted in TSWV-Infected Tomato (Solanum lycopersicum) with or without Sw-5 Gene
title_fullStr The Tomato spotted wilt virus (TSWV) Genome is Differentially Targeted in TSWV-Infected Tomato (Solanum lycopersicum) with or without Sw-5 Gene
title_full_unstemmed The Tomato spotted wilt virus (TSWV) Genome is Differentially Targeted in TSWV-Infected Tomato (Solanum lycopersicum) with or without Sw-5 Gene
title_short The Tomato spotted wilt virus (TSWV) Genome is Differentially Targeted in TSWV-Infected Tomato (Solanum lycopersicum) with or without Sw-5 Gene
title_sort tomato spotted wilt virus (tswv) genome is differentially targeted in tswv-infected tomato (solanum lycopersicum) with or without sw-5 gene
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7232525/
https://www.ncbi.nlm.nih.gov/pubmed/32224858
http://dx.doi.org/10.3390/v12040363
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