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Effects of an injectable long-acting formulation of ivermectin on Onchocerca ochengi in zebu cattle

The availability of a safe macrofilaricidal drug would help to accelerate onchocerciasis elimination. A trial was conducted in Cameroon to evaluate the effects of a subcutaneous injectable long-acting formulation of ivermectin (LAFI) on the microfilariae (mf) and adult stages of Onchocerca ochengi....

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Autores principales: Boussinesq, Michel, Enyong, Peter, Chounna-Ndongmo, Patrick, Njouendou, Abdel-Jelil, Pion, Sébastien David, Rech, Anthony, Roberge, Christophe, Gaudriault, Georges, Wanji, Samuel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: EDP Sciences 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7233116/
https://www.ncbi.nlm.nih.gov/pubmed/32420864
http://dx.doi.org/10.1051/parasite/2020036
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author Boussinesq, Michel
Enyong, Peter
Chounna-Ndongmo, Patrick
Njouendou, Abdel-Jelil
Pion, Sébastien David
Rech, Anthony
Roberge, Christophe
Gaudriault, Georges
Wanji, Samuel
author_facet Boussinesq, Michel
Enyong, Peter
Chounna-Ndongmo, Patrick
Njouendou, Abdel-Jelil
Pion, Sébastien David
Rech, Anthony
Roberge, Christophe
Gaudriault, Georges
Wanji, Samuel
author_sort Boussinesq, Michel
collection PubMed
description The availability of a safe macrofilaricidal drug would help to accelerate onchocerciasis elimination. A trial was conducted in Cameroon to evaluate the effects of a subcutaneous injectable long-acting formulation of ivermectin (LAFI) on the microfilariae (mf) and adult stages of Onchocerca ochengi. Ten zebu cattle naturally infected with the parasite were injected subcutaneously with either 500 mg (group A, N = 4), or 1000 mg long-acting ivermectin (group B, N = 4) or the vehicle (group C, N = 2). Skin samples were collected from each animal before, and 6, 12, and 24 months after treatment to measure microfilarial densities (MFDs). Nodules excised before, and 6 and 12 months after treatment were examined histologically to assess the adult worms’ viability and reproductive status. Blood samples were collected at pre-determined time-points to obtain pharmacokinetic data. Before treatment, the average O. ochengi MFDs were similar in the three groups. Six months after treatment, all animals in groups A and B were free of skin mf, whereas those in group C still showed high MFDs (mean = 324.5 mf/g). Only one ivermectin-treated animal (belonging to group A) had skin mf 12 months after treatment (0.9 mf/g). At 24 months, another animal in group A showed skin mf (10.0 mf/g). The histologic examination of nodules at 6 and 12 months showed that LAFI was not macrofilaricidal but had a strong effect on embryogenesis. The new LAFI regimen might be an additional tool to accelerate the elimination of human onchocerciasis in specific settings.
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spelling pubmed-72331162020-05-19 Effects of an injectable long-acting formulation of ivermectin on Onchocerca ochengi in zebu cattle Boussinesq, Michel Enyong, Peter Chounna-Ndongmo, Patrick Njouendou, Abdel-Jelil Pion, Sébastien David Rech, Anthony Roberge, Christophe Gaudriault, Georges Wanji, Samuel Parasite Research Article The availability of a safe macrofilaricidal drug would help to accelerate onchocerciasis elimination. A trial was conducted in Cameroon to evaluate the effects of a subcutaneous injectable long-acting formulation of ivermectin (LAFI) on the microfilariae (mf) and adult stages of Onchocerca ochengi. Ten zebu cattle naturally infected with the parasite were injected subcutaneously with either 500 mg (group A, N = 4), or 1000 mg long-acting ivermectin (group B, N = 4) or the vehicle (group C, N = 2). Skin samples were collected from each animal before, and 6, 12, and 24 months after treatment to measure microfilarial densities (MFDs). Nodules excised before, and 6 and 12 months after treatment were examined histologically to assess the adult worms’ viability and reproductive status. Blood samples were collected at pre-determined time-points to obtain pharmacokinetic data. Before treatment, the average O. ochengi MFDs were similar in the three groups. Six months after treatment, all animals in groups A and B were free of skin mf, whereas those in group C still showed high MFDs (mean = 324.5 mf/g). Only one ivermectin-treated animal (belonging to group A) had skin mf 12 months after treatment (0.9 mf/g). At 24 months, another animal in group A showed skin mf (10.0 mf/g). The histologic examination of nodules at 6 and 12 months showed that LAFI was not macrofilaricidal but had a strong effect on embryogenesis. The new LAFI regimen might be an additional tool to accelerate the elimination of human onchocerciasis in specific settings. EDP Sciences 2020-05-18 /pmc/articles/PMC7233116/ /pubmed/32420864 http://dx.doi.org/10.1051/parasite/2020036 Text en © M. Boussinesq et al., published by EDP Sciences, 2020 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Boussinesq, Michel
Enyong, Peter
Chounna-Ndongmo, Patrick
Njouendou, Abdel-Jelil
Pion, Sébastien David
Rech, Anthony
Roberge, Christophe
Gaudriault, Georges
Wanji, Samuel
Effects of an injectable long-acting formulation of ivermectin on Onchocerca ochengi in zebu cattle
title Effects of an injectable long-acting formulation of ivermectin on Onchocerca ochengi in zebu cattle
title_full Effects of an injectable long-acting formulation of ivermectin on Onchocerca ochengi in zebu cattle
title_fullStr Effects of an injectable long-acting formulation of ivermectin on Onchocerca ochengi in zebu cattle
title_full_unstemmed Effects of an injectable long-acting formulation of ivermectin on Onchocerca ochengi in zebu cattle
title_short Effects of an injectable long-acting formulation of ivermectin on Onchocerca ochengi in zebu cattle
title_sort effects of an injectable long-acting formulation of ivermectin on onchocerca ochengi in zebu cattle
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7233116/
https://www.ncbi.nlm.nih.gov/pubmed/32420864
http://dx.doi.org/10.1051/parasite/2020036
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