Sequence mismatch in PCR probes may mask the COVID-19 detection in Nepal

• Most of the COVID-19 cases in Nepal are in the Southern districts of Nepal bordering India with travel histories to India. • Very few positive cases of COVID-19 are detected in Nepal which could either be due to early national lockdown. • Low PCR positivity rates could also be due to inefficiency...

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Detalles Bibliográficos
Autores principales: Rana, Divya RSJB, Pokhrel, Nischal
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Ltd. 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7233248/
https://www.ncbi.nlm.nih.gov/pubmed/32425334
http://dx.doi.org/10.1016/j.mcp.2020.101599
Descripción
Sumario:• Most of the COVID-19 cases in Nepal are in the Southern districts of Nepal bordering India with travel histories to India. • Very few positive cases of COVID-19 are detected in Nepal which could either be due to early national lockdown. • Low PCR positivity rates could also be due to inefficiency of the PCR methods. • Whole genomes of 93 clinical samples from COVID-19 patients were analyzed to find the primer and probe binding sites. • Mutations in probe binding sites were found which could impact PCR efficiency resulting in false negative results.

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