Sequence mismatch in PCR probes may mask the COVID-19 detection in Nepal

• Most of the COVID-19 cases in Nepal are in the Southern districts of Nepal bordering India with travel histories to India. • Very few positive cases of COVID-19 are detected in Nepal which could either be due to early national lockdown. • Low PCR positivity rates could also be due to inefficiency...

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Detalles Bibliográficos
Autores principales: Rana, Divya RSJB, Pokhrel, Nischal
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Ltd. 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7233248/
https://www.ncbi.nlm.nih.gov/pubmed/32425334
http://dx.doi.org/10.1016/j.mcp.2020.101599
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author Rana, Divya RSJB
Pokhrel, Nischal
author_facet Rana, Divya RSJB
Pokhrel, Nischal
author_sort Rana, Divya RSJB
collection PubMed
description • Most of the COVID-19 cases in Nepal are in the Southern districts of Nepal bordering India with travel histories to India. • Very few positive cases of COVID-19 are detected in Nepal which could either be due to early national lockdown. • Low PCR positivity rates could also be due to inefficiency of the PCR methods. • Whole genomes of 93 clinical samples from COVID-19 patients were analyzed to find the primer and probe binding sites. • Mutations in probe binding sites were found which could impact PCR efficiency resulting in false negative results.
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spelling pubmed-72332482020-05-18 Sequence mismatch in PCR probes may mask the COVID-19 detection in Nepal Rana, Divya RSJB Pokhrel, Nischal Mol Cell Probes Article • Most of the COVID-19 cases in Nepal are in the Southern districts of Nepal bordering India with travel histories to India. • Very few positive cases of COVID-19 are detected in Nepal which could either be due to early national lockdown. • Low PCR positivity rates could also be due to inefficiency of the PCR methods. • Whole genomes of 93 clinical samples from COVID-19 patients were analyzed to find the primer and probe binding sites. • Mutations in probe binding sites were found which could impact PCR efficiency resulting in false negative results. Elsevier Ltd. 2020-10 2020-05-18 /pmc/articles/PMC7233248/ /pubmed/32425334 http://dx.doi.org/10.1016/j.mcp.2020.101599 Text en © 2020 Elsevier Ltd. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Rana, Divya RSJB
Pokhrel, Nischal
Sequence mismatch in PCR probes may mask the COVID-19 detection in Nepal
title Sequence mismatch in PCR probes may mask the COVID-19 detection in Nepal
title_full Sequence mismatch in PCR probes may mask the COVID-19 detection in Nepal
title_fullStr Sequence mismatch in PCR probes may mask the COVID-19 detection in Nepal
title_full_unstemmed Sequence mismatch in PCR probes may mask the COVID-19 detection in Nepal
title_short Sequence mismatch in PCR probes may mask the COVID-19 detection in Nepal
title_sort sequence mismatch in pcr probes may mask the covid-19 detection in nepal
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7233248/
https://www.ncbi.nlm.nih.gov/pubmed/32425334
http://dx.doi.org/10.1016/j.mcp.2020.101599
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