Enzyme- and gene-specific biases in reverse transcription of RNA raise concerns for evaluating gene expression

Reverse transcription is the first step of most analyses of gene expression, yet the quantitative biases it introduces are largely overlooked. Following a series of purpose-designed systematic experiments we cherry-pick examples of various biases introduced by reverse transcription, and alert the “g...

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Autores principales: Minshall, Nicola, Git, Anna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7235240/
https://www.ncbi.nlm.nih.gov/pubmed/32424137
http://dx.doi.org/10.1038/s41598-020-65005-0
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author Minshall, Nicola
Git, Anna
author_facet Minshall, Nicola
Git, Anna
author_sort Minshall, Nicola
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description Reverse transcription is the first step of most analyses of gene expression, yet the quantitative biases it introduces are largely overlooked. Following a series of purpose-designed systematic experiments we cherry-pick examples of various biases introduced by reverse transcription, and alert the “gene expression community” to the pitfalls and improved practice of this fundamental technique.
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spelling pubmed-72352402020-05-29 Enzyme- and gene-specific biases in reverse transcription of RNA raise concerns for evaluating gene expression Minshall, Nicola Git, Anna Sci Rep Article Reverse transcription is the first step of most analyses of gene expression, yet the quantitative biases it introduces are largely overlooked. Following a series of purpose-designed systematic experiments we cherry-pick examples of various biases introduced by reverse transcription, and alert the “gene expression community” to the pitfalls and improved practice of this fundamental technique. Nature Publishing Group UK 2020-05-18 /pmc/articles/PMC7235240/ /pubmed/32424137 http://dx.doi.org/10.1038/s41598-020-65005-0 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Minshall, Nicola
Git, Anna
Enzyme- and gene-specific biases in reverse transcription of RNA raise concerns for evaluating gene expression
title Enzyme- and gene-specific biases in reverse transcription of RNA raise concerns for evaluating gene expression
title_full Enzyme- and gene-specific biases in reverse transcription of RNA raise concerns for evaluating gene expression
title_fullStr Enzyme- and gene-specific biases in reverse transcription of RNA raise concerns for evaluating gene expression
title_full_unstemmed Enzyme- and gene-specific biases in reverse transcription of RNA raise concerns for evaluating gene expression
title_short Enzyme- and gene-specific biases in reverse transcription of RNA raise concerns for evaluating gene expression
title_sort enzyme- and gene-specific biases in reverse transcription of rna raise concerns for evaluating gene expression
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7235240/
https://www.ncbi.nlm.nih.gov/pubmed/32424137
http://dx.doi.org/10.1038/s41598-020-65005-0
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