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Enzyme- and gene-specific biases in reverse transcription of RNA raise concerns for evaluating gene expression
Reverse transcription is the first step of most analyses of gene expression, yet the quantitative biases it introduces are largely overlooked. Following a series of purpose-designed systematic experiments we cherry-pick examples of various biases introduced by reverse transcription, and alert the “g...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Nature Publishing Group UK
2020
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7235240/ https://www.ncbi.nlm.nih.gov/pubmed/32424137 http://dx.doi.org/10.1038/s41598-020-65005-0 |