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Immunorthodontics: in vivo gene expression of orthodontic tooth movement

Orthodontic tooth movement (OTM) is a “sterile” inflammatory process. The present study aimed to reveal the underlying biological mechanisms, by studying the force associated-gene expression changes, in a time-dependent manner. Ni-Ti springs were set to move the upper 1(st)-molar in C57BL/6 mice. OT...

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Autores principales: Klein, Yehuda, Fleissig, Omer, Polak, David, Barenholz, Yechezkel, Mandelboim, Ofer, Chaushu, Stella
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7235241/
https://www.ncbi.nlm.nih.gov/pubmed/32424121
http://dx.doi.org/10.1038/s41598-020-65089-8
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author Klein, Yehuda
Fleissig, Omer
Polak, David
Barenholz, Yechezkel
Mandelboim, Ofer
Chaushu, Stella
author_facet Klein, Yehuda
Fleissig, Omer
Polak, David
Barenholz, Yechezkel
Mandelboim, Ofer
Chaushu, Stella
author_sort Klein, Yehuda
collection PubMed
description Orthodontic tooth movement (OTM) is a “sterile” inflammatory process. The present study aimed to reveal the underlying biological mechanisms, by studying the force associated-gene expression changes, in a time-dependent manner. Ni-Ti springs were set to move the upper 1(st)-molar in C57BL/6 mice. OTM was measured by μCT. Total-RNA was extracted from tissue blocks at 1,3,7 and 14-days post force application, and from two control groups: naïve and inactivated spring. Gene-expression profiles were generated by next-generation-RNA-sequencing. Gene Set Enrichment Analysis, K-means algorithm and Ingenuity pathway analysis were used for data interpretation. Genes of interest were validated with qRT-PCR. A total of 3075 differentially expressed genes (DEGs) were identified, with the greatest number at day 3. Two distinct clusters patterns were recognized: those in which DEGs peaked in the first days and declined thereafter (tissue degradation, phagocytosis, leukocyte extravasation, innate and adaptive immune system responses), and those in which DEGs were initially down-regulated and increased at day 14 (cell proliferation and migration, cytoskeletal rearrangement, tissue homeostasis, angiogenesis). The uncovering of novel innate and adaptive immune processes in OTM led us to propose a new term “Immunorthodontics”. This genomic data can serve as a platform for OTM modulation future approaches.
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spelling pubmed-72352412020-05-29 Immunorthodontics: in vivo gene expression of orthodontic tooth movement Klein, Yehuda Fleissig, Omer Polak, David Barenholz, Yechezkel Mandelboim, Ofer Chaushu, Stella Sci Rep Article Orthodontic tooth movement (OTM) is a “sterile” inflammatory process. The present study aimed to reveal the underlying biological mechanisms, by studying the force associated-gene expression changes, in a time-dependent manner. Ni-Ti springs were set to move the upper 1(st)-molar in C57BL/6 mice. OTM was measured by μCT. Total-RNA was extracted from tissue blocks at 1,3,7 and 14-days post force application, and from two control groups: naïve and inactivated spring. Gene-expression profiles were generated by next-generation-RNA-sequencing. Gene Set Enrichment Analysis, K-means algorithm and Ingenuity pathway analysis were used for data interpretation. Genes of interest were validated with qRT-PCR. A total of 3075 differentially expressed genes (DEGs) were identified, with the greatest number at day 3. Two distinct clusters patterns were recognized: those in which DEGs peaked in the first days and declined thereafter (tissue degradation, phagocytosis, leukocyte extravasation, innate and adaptive immune system responses), and those in which DEGs were initially down-regulated and increased at day 14 (cell proliferation and migration, cytoskeletal rearrangement, tissue homeostasis, angiogenesis). The uncovering of novel innate and adaptive immune processes in OTM led us to propose a new term “Immunorthodontics”. This genomic data can serve as a platform for OTM modulation future approaches. Nature Publishing Group UK 2020-05-18 /pmc/articles/PMC7235241/ /pubmed/32424121 http://dx.doi.org/10.1038/s41598-020-65089-8 Text en © The Author(s) 2020, corrected publication 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Klein, Yehuda
Fleissig, Omer
Polak, David
Barenholz, Yechezkel
Mandelboim, Ofer
Chaushu, Stella
Immunorthodontics: in vivo gene expression of orthodontic tooth movement
title Immunorthodontics: in vivo gene expression of orthodontic tooth movement
title_full Immunorthodontics: in vivo gene expression of orthodontic tooth movement
title_fullStr Immunorthodontics: in vivo gene expression of orthodontic tooth movement
title_full_unstemmed Immunorthodontics: in vivo gene expression of orthodontic tooth movement
title_short Immunorthodontics: in vivo gene expression of orthodontic tooth movement
title_sort immunorthodontics: in vivo gene expression of orthodontic tooth movement
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7235241/
https://www.ncbi.nlm.nih.gov/pubmed/32424121
http://dx.doi.org/10.1038/s41598-020-65089-8
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