Visualization of PS/γ-Secretase Activity in Living Cells

A change in Presenilin (PS)/γ-secretase activity is linked to essential biological events as well as to the progression of many diseases. However, not much is known about how PS/γ-secretase activity is spatiotemporally regulated in cells. One of the limitations is lack of tools to directly monitor d...

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Autores principales: Maesako, Masato, Sekula, Nicole M., Aristarkhova, Anna, Feschenko, Polina, Anderson, Lauren C., Berezovska, Oksana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7235286/
https://www.ncbi.nlm.nih.gov/pubmed/32438286
http://dx.doi.org/10.1016/j.isci.2020.101139
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author Maesako, Masato
Sekula, Nicole M.
Aristarkhova, Anna
Feschenko, Polina
Anderson, Lauren C.
Berezovska, Oksana
author_facet Maesako, Masato
Sekula, Nicole M.
Aristarkhova, Anna
Feschenko, Polina
Anderson, Lauren C.
Berezovska, Oksana
author_sort Maesako, Masato
collection PubMed
description A change in Presenilin (PS)/γ-secretase activity is linked to essential biological events as well as to the progression of many diseases. However, not much is known about how PS/γ-secretase activity is spatiotemporally regulated in cells. One of the limitations is lack of tools to directly monitor dynamic behavior of the PS/γ-secretase in intact/live cells. Here we present successful development and validation of the Förster resonance energy transfer (FRET)-based biosensors that enable quantitative monitoring of endogenous PS/γ-secretase activity in live cells longitudinally on a cell-by-cell basis. Using these FRET biosensors, we uncovered that PS/γ-secretase activity is heterogeneously regulated among live neurons.
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spelling pubmed-72352862020-05-20 Visualization of PS/γ-Secretase Activity in Living Cells Maesako, Masato Sekula, Nicole M. Aristarkhova, Anna Feschenko, Polina Anderson, Lauren C. Berezovska, Oksana iScience Article A change in Presenilin (PS)/γ-secretase activity is linked to essential biological events as well as to the progression of many diseases. However, not much is known about how PS/γ-secretase activity is spatiotemporally regulated in cells. One of the limitations is lack of tools to directly monitor dynamic behavior of the PS/γ-secretase in intact/live cells. Here we present successful development and validation of the Förster resonance energy transfer (FRET)-based biosensors that enable quantitative monitoring of endogenous PS/γ-secretase activity in live cells longitudinally on a cell-by-cell basis. Using these FRET biosensors, we uncovered that PS/γ-secretase activity is heterogeneously regulated among live neurons. Elsevier 2020-05-07 /pmc/articles/PMC7235286/ /pubmed/32438286 http://dx.doi.org/10.1016/j.isci.2020.101139 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Maesako, Masato
Sekula, Nicole M.
Aristarkhova, Anna
Feschenko, Polina
Anderson, Lauren C.
Berezovska, Oksana
Visualization of PS/γ-Secretase Activity in Living Cells
title Visualization of PS/γ-Secretase Activity in Living Cells
title_full Visualization of PS/γ-Secretase Activity in Living Cells
title_fullStr Visualization of PS/γ-Secretase Activity in Living Cells
title_full_unstemmed Visualization of PS/γ-Secretase Activity in Living Cells
title_short Visualization of PS/γ-Secretase Activity in Living Cells
title_sort visualization of ps/γ-secretase activity in living cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7235286/
https://www.ncbi.nlm.nih.gov/pubmed/32438286
http://dx.doi.org/10.1016/j.isci.2020.101139
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AT andersonlaurenc visualizationofpsgsecretaseactivityinlivingcells
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