Identification of Novel Molecular Network Expression in Acute Myocardial Infarction

BACKGROUND: In the current study, we aimed to analyze the hypothesis that human myocardial-specific extracellular RNAs expression could be used for acute myocardial injury(AMI) diagnosis. METHODOLOGY: We used bioinformatics’ analysis to identify RNAs linked to ubiquitin system and specific to AMI, n...

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Detalles Bibliográficos
Autores principales: Matboli, Marwa, Shafei, Ayman E., Agwa, Sara H.A., Elzahy, Sherif Sammir, Anwar, Ahmed K., Mansour, Amr R., Gaber, Ahmed I., Said, Ali E.A., Lwis, Paula, Hamdy, Marwa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Bentham Science Publishers 2019
Materias:
Genomics
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7235391/
https://www.ncbi.nlm.nih.gov/pubmed/32476991
http://dx.doi.org/10.2174/1389202920666190820142043
Descripción
Sumario:BACKGROUND: In the current study, we aimed to analyze the hypothesis that human myocardial-specific extracellular RNAs expression could be used for acute myocardial injury(AMI) diagnosis. METHODOLOGY: We used bioinformatics’ analysis to identify RNAs linked to ubiquitin system and specific to AMI, named, (lncRNA-RP11-175K6.1), (LOC101927740), microRNA-106b-5p (miR-106b-5p) and Anaphase, promoting complex 11 (ANapc11mRNA). We measured the serum expression of the chosen RNAs in 69 individuals with acute coronary syndromes, 31 individuals with angina pectoris without MI and non-cardiac chest pain and 31 healthy control individuals by real-time reverse-transcription PCR. RESULTS: Our study revealed a significant decrease in both lncRNA-RP11-175K6.1 and ANapc11mRNA expression of in the sera samples of AMI patients compared to that of the two control groups alongside with significant upregulation of miR-106b-5p. CONCLUSION: Of note, the investigated serum RNAs decrease the false discovery rate of AMI to 3.2%.

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