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Long Noncoding RNA WT1-AS Inhibit Cell Malignancy via miR-494-3p in Glioma

Primary brain tumors are a rare occurrence in comparison to other malignancies, the most predominant form being glioma. Commonly, exposure to ionizing radiations and inheritance of associated conditions such a neurofibromatosis and tuberous sclerosis are the most common causes of development of glio...

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Autores principales: Qiu, Guangting, Tong, Wenjie, Jiang, Chenghao, Xie, Qingsong, Zou, Jingfang, Luo, Cong, Zhao, Jianwei, Zhang, Lu, Zhao, Jiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7235650/
https://www.ncbi.nlm.nih.gov/pubmed/32419643
http://dx.doi.org/10.1177/1533033820919759
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author Qiu, Guangting
Tong, Wenjie
Jiang, Chenghao
Xie, Qingsong
Zou, Jingfang
Luo, Cong
Zhao, Jianwei
Zhang, Lu
Zhao, Jiang
author_facet Qiu, Guangting
Tong, Wenjie
Jiang, Chenghao
Xie, Qingsong
Zou, Jingfang
Luo, Cong
Zhao, Jianwei
Zhang, Lu
Zhao, Jiang
author_sort Qiu, Guangting
collection PubMed
description Primary brain tumors are a rare occurrence in comparison to other malignancies, the most predominant form being glioma. Commonly, exposure to ionizing radiations and inheritance of associated conditions such a neurofibromatosis and tuberous sclerosis are the most common causes of development of glioma. However, understanding of the molecular mechanisms that drive glioma development is limited. We explore the role of aberration of microRNA namely miR-494-3p through long noncoding RNA WT1-AS in the development of gliomas. In this study, we found that, levels of WT1-AS were significantly reduced in glioma tissues and cell lines. The miR-494-3p levels were negatively correlated with WT1-AS levels. The cellular proliferation and invasiveness decreased in WT1-AS transfected cell lines. Further the half maximal inhibitory concentration (IC(50)) of chemotherapeutic agent temozolomide was significantly reduced in the presence of WT1-AS. The cotransfection of WT1-AS and miR-494-3p reduced activation of phospho-AKT (p-AKT). Expression of miR-494-3p is modulated by binding to long noncoding RNA WT1-AS. Deregulation of WT1-AS leads to aberrant expression of miR-494-3p leading to hyperactivation of AKT. This malformation may result in altering protective immune responses in malignancies. Targeting of WT1-AS, miR-494-3p, and AKT may be novel therapeutic options in treatment of glioma.
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spelling pubmed-72356502020-06-09 Long Noncoding RNA WT1-AS Inhibit Cell Malignancy via miR-494-3p in Glioma Qiu, Guangting Tong, Wenjie Jiang, Chenghao Xie, Qingsong Zou, Jingfang Luo, Cong Zhao, Jianwei Zhang, Lu Zhao, Jiang Technol Cancer Res Treat Original Article Primary brain tumors are a rare occurrence in comparison to other malignancies, the most predominant form being glioma. Commonly, exposure to ionizing radiations and inheritance of associated conditions such a neurofibromatosis and tuberous sclerosis are the most common causes of development of glioma. However, understanding of the molecular mechanisms that drive glioma development is limited. We explore the role of aberration of microRNA namely miR-494-3p through long noncoding RNA WT1-AS in the development of gliomas. In this study, we found that, levels of WT1-AS were significantly reduced in glioma tissues and cell lines. The miR-494-3p levels were negatively correlated with WT1-AS levels. The cellular proliferation and invasiveness decreased in WT1-AS transfected cell lines. Further the half maximal inhibitory concentration (IC(50)) of chemotherapeutic agent temozolomide was significantly reduced in the presence of WT1-AS. The cotransfection of WT1-AS and miR-494-3p reduced activation of phospho-AKT (p-AKT). Expression of miR-494-3p is modulated by binding to long noncoding RNA WT1-AS. Deregulation of WT1-AS leads to aberrant expression of miR-494-3p leading to hyperactivation of AKT. This malformation may result in altering protective immune responses in malignancies. Targeting of WT1-AS, miR-494-3p, and AKT may be novel therapeutic options in treatment of glioma. SAGE Publications 2020-05-18 /pmc/articles/PMC7235650/ /pubmed/32419643 http://dx.doi.org/10.1177/1533033820919759 Text en © The Author(s) 2020 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Original Article
Qiu, Guangting
Tong, Wenjie
Jiang, Chenghao
Xie, Qingsong
Zou, Jingfang
Luo, Cong
Zhao, Jianwei
Zhang, Lu
Zhao, Jiang
Long Noncoding RNA WT1-AS Inhibit Cell Malignancy via miR-494-3p in Glioma
title Long Noncoding RNA WT1-AS Inhibit Cell Malignancy via miR-494-3p in Glioma
title_full Long Noncoding RNA WT1-AS Inhibit Cell Malignancy via miR-494-3p in Glioma
title_fullStr Long Noncoding RNA WT1-AS Inhibit Cell Malignancy via miR-494-3p in Glioma
title_full_unstemmed Long Noncoding RNA WT1-AS Inhibit Cell Malignancy via miR-494-3p in Glioma
title_short Long Noncoding RNA WT1-AS Inhibit Cell Malignancy via miR-494-3p in Glioma
title_sort long noncoding rna wt1-as inhibit cell malignancy via mir-494-3p in glioma
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7235650/
https://www.ncbi.nlm.nih.gov/pubmed/32419643
http://dx.doi.org/10.1177/1533033820919759
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