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Design and Fabrication of Capillary-Driven Flow Device for Point-Of-Care Diagnostics

Point-of-care (POC) diagnostics enables the diagnosis and monitoring of patients from the clinic or their home. Ideally, POC devices should be compact, portable and operatable without the requirement of expertise or complex fluid mechanical controls. This paper showcases a chip-and-dip device, which...

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Detalles Bibliográficos
Autores principales: Hassan, Sammer-ul, Zhang, Xunli
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7235737/
https://www.ncbi.nlm.nih.gov/pubmed/32326641
http://dx.doi.org/10.3390/bios10040039
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author Hassan, Sammer-ul
Zhang, Xunli
author_facet Hassan, Sammer-ul
Zhang, Xunli
author_sort Hassan, Sammer-ul
collection PubMed
description Point-of-care (POC) diagnostics enables the diagnosis and monitoring of patients from the clinic or their home. Ideally, POC devices should be compact, portable and operatable without the requirement of expertise or complex fluid mechanical controls. This paper showcases a chip-and-dip device, which works on the principle of capillary-driven flow microfluidics and allows analytes’ detection by multiple microchannels in a single microchip via smartphone imaging. The chip-and-dip device, fabricated with inexpensive materials, works by simply dipping the reagents-coated microchip consisting of microchannels into a fluidic sample. The sample is loaded into the microchannels via capillary action and reacts with the reagents to produce a colourimetric signal. Unlike dipstick tests, this device allows the loading of bacterial/pathogenic samples for antimicrobial testing. A single device can be coated with multiple reagents, and more analytes can be detected in one sample. This platform could be used for a wide variety of assays. Here, we show the design, fabrication and working principle of the chip-and-dip flow device along with a specific application consisting in the determination of β-lactamase activity and cortisol. The simplicity, robustness and multiplexing capability of the chip-and-dip device will allow it to be used for POC diagnostics.
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spelling pubmed-72357372020-05-22 Design and Fabrication of Capillary-Driven Flow Device for Point-Of-Care Diagnostics Hassan, Sammer-ul Zhang, Xunli Biosensors (Basel) Communication Point-of-care (POC) diagnostics enables the diagnosis and monitoring of patients from the clinic or their home. Ideally, POC devices should be compact, portable and operatable without the requirement of expertise or complex fluid mechanical controls. This paper showcases a chip-and-dip device, which works on the principle of capillary-driven flow microfluidics and allows analytes’ detection by multiple microchannels in a single microchip via smartphone imaging. The chip-and-dip device, fabricated with inexpensive materials, works by simply dipping the reagents-coated microchip consisting of microchannels into a fluidic sample. The sample is loaded into the microchannels via capillary action and reacts with the reagents to produce a colourimetric signal. Unlike dipstick tests, this device allows the loading of bacterial/pathogenic samples for antimicrobial testing. A single device can be coated with multiple reagents, and more analytes can be detected in one sample. This platform could be used for a wide variety of assays. Here, we show the design, fabrication and working principle of the chip-and-dip flow device along with a specific application consisting in the determination of β-lactamase activity and cortisol. The simplicity, robustness and multiplexing capability of the chip-and-dip device will allow it to be used for POC diagnostics. MDPI 2020-04-15 /pmc/articles/PMC7235737/ /pubmed/32326641 http://dx.doi.org/10.3390/bios10040039 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Hassan, Sammer-ul
Zhang, Xunli
Design and Fabrication of Capillary-Driven Flow Device for Point-Of-Care Diagnostics
title Design and Fabrication of Capillary-Driven Flow Device for Point-Of-Care Diagnostics
title_full Design and Fabrication of Capillary-Driven Flow Device for Point-Of-Care Diagnostics
title_fullStr Design and Fabrication of Capillary-Driven Flow Device for Point-Of-Care Diagnostics
title_full_unstemmed Design and Fabrication of Capillary-Driven Flow Device for Point-Of-Care Diagnostics
title_short Design and Fabrication of Capillary-Driven Flow Device for Point-Of-Care Diagnostics
title_sort design and fabrication of capillary-driven flow device for point-of-care diagnostics
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7235737/
https://www.ncbi.nlm.nih.gov/pubmed/32326641
http://dx.doi.org/10.3390/bios10040039
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