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Polyphasic Validation of a Nisin-Biogel to Control Canine Periodontal Disease

Background: Periodontal disease (PD) is a highly prevalent inflammatory disease in dogs. This disease is initiated by a polymicrobial biofilm on the teeth surface, whose control includes its prevention and removal. Recently, it was shown that nisin displays antimicrobial activity against canine PD-r...

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Autores principales: Cunha, Eva, Freitas, Ferdinando Bernardino, São Braz, Berta, Moreira da Silva, Jorge, Tavares, Luís, Veiga, Ana Salomé, Oliveira, Manuela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7235766/
https://www.ncbi.nlm.nih.gov/pubmed/32295175
http://dx.doi.org/10.3390/antibiotics9040180
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author Cunha, Eva
Freitas, Ferdinando Bernardino
São Braz, Berta
Moreira da Silva, Jorge
Tavares, Luís
Veiga, Ana Salomé
Oliveira, Manuela
author_facet Cunha, Eva
Freitas, Ferdinando Bernardino
São Braz, Berta
Moreira da Silva, Jorge
Tavares, Luís
Veiga, Ana Salomé
Oliveira, Manuela
author_sort Cunha, Eva
collection PubMed
description Background: Periodontal disease (PD) is a highly prevalent inflammatory disease in dogs. This disease is initiated by a polymicrobial biofilm on the teeth surface, whose control includes its prevention and removal. Recently, it was shown that nisin displays antimicrobial activity against canine PD-related bacteria. Moreover, guar gum biogel has shown to be a promising topical delivery system for nisin. Methods: In this study we aimed to evaluate the antimicrobial activity of the nisin-biogel in the presence of canine saliva and after a 24-month storage, at different conditions, using a canine oral enterococci collection. We also studied the nisin-biogel cytotoxicity using a Vero cell line and canine primary intestinal fibroblasts. Results: The presence of saliva hampers nisin-biogel antimicrobial activity, and higher nisin concentrations were required for an effective activity. A significant reduction (p ≤ 0.05) in inhibitory activity was observed for nisin-biogel solutions stored at 37 °C, over a 24-month period, which was not observed with the other conditions. The nisin-biogel showed no cytotoxicity against the cells tested at concentrations up to 200 µg/mL. Conclusions: Our results confirmed the potential of the nisin-biogel for canine PD control, supporting the development of an in vivo clinical trial.
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spelling pubmed-72357662020-05-22 Polyphasic Validation of a Nisin-Biogel to Control Canine Periodontal Disease Cunha, Eva Freitas, Ferdinando Bernardino São Braz, Berta Moreira da Silva, Jorge Tavares, Luís Veiga, Ana Salomé Oliveira, Manuela Antibiotics (Basel) Article Background: Periodontal disease (PD) is a highly prevalent inflammatory disease in dogs. This disease is initiated by a polymicrobial biofilm on the teeth surface, whose control includes its prevention and removal. Recently, it was shown that nisin displays antimicrobial activity against canine PD-related bacteria. Moreover, guar gum biogel has shown to be a promising topical delivery system for nisin. Methods: In this study we aimed to evaluate the antimicrobial activity of the nisin-biogel in the presence of canine saliva and after a 24-month storage, at different conditions, using a canine oral enterococci collection. We also studied the nisin-biogel cytotoxicity using a Vero cell line and canine primary intestinal fibroblasts. Results: The presence of saliva hampers nisin-biogel antimicrobial activity, and higher nisin concentrations were required for an effective activity. A significant reduction (p ≤ 0.05) in inhibitory activity was observed for nisin-biogel solutions stored at 37 °C, over a 24-month period, which was not observed with the other conditions. The nisin-biogel showed no cytotoxicity against the cells tested at concentrations up to 200 µg/mL. Conclusions: Our results confirmed the potential of the nisin-biogel for canine PD control, supporting the development of an in vivo clinical trial. MDPI 2020-04-14 /pmc/articles/PMC7235766/ /pubmed/32295175 http://dx.doi.org/10.3390/antibiotics9040180 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Cunha, Eva
Freitas, Ferdinando Bernardino
São Braz, Berta
Moreira da Silva, Jorge
Tavares, Luís
Veiga, Ana Salomé
Oliveira, Manuela
Polyphasic Validation of a Nisin-Biogel to Control Canine Periodontal Disease
title Polyphasic Validation of a Nisin-Biogel to Control Canine Periodontal Disease
title_full Polyphasic Validation of a Nisin-Biogel to Control Canine Periodontal Disease
title_fullStr Polyphasic Validation of a Nisin-Biogel to Control Canine Periodontal Disease
title_full_unstemmed Polyphasic Validation of a Nisin-Biogel to Control Canine Periodontal Disease
title_short Polyphasic Validation of a Nisin-Biogel to Control Canine Periodontal Disease
title_sort polyphasic validation of a nisin-biogel to control canine periodontal disease
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7235766/
https://www.ncbi.nlm.nih.gov/pubmed/32295175
http://dx.doi.org/10.3390/antibiotics9040180
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