Cargando…

Increased Circulating Levels of Tissue-Type Plasminogen Activator Are Associated with the Risk of Spontaneous Abortion During the First Trimester of Pregnancy

Spontaneous abortion is a common complication in early pregnancy, with an incidence of around 20%. Ultrasound scan and measurement of human chorionic gonadotropin are used to identify patients at risk of spontaneous abortion; however, there is a clinical need to find new biomarkers to prospectively...

Descripción completa

Detalles Bibliográficos
Autores principales: Monteiro, Lara J., Varas-Godoy, Manuel, Acuña-Gallardo, Stephanie, Correa, Paula, Passalacqua, Gianluca, Monckeberg, Max, Rice, Gregory E., Illanes, Sebastián E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7235768/
https://www.ncbi.nlm.nih.gov/pubmed/32244842
http://dx.doi.org/10.3390/diagnostics10040197
_version_ 1783536031335710720
author Monteiro, Lara J.
Varas-Godoy, Manuel
Acuña-Gallardo, Stephanie
Correa, Paula
Passalacqua, Gianluca
Monckeberg, Max
Rice, Gregory E.
Illanes, Sebastián E.
author_facet Monteiro, Lara J.
Varas-Godoy, Manuel
Acuña-Gallardo, Stephanie
Correa, Paula
Passalacqua, Gianluca
Monckeberg, Max
Rice, Gregory E.
Illanes, Sebastián E.
author_sort Monteiro, Lara J.
collection PubMed
description Spontaneous abortion is a common complication in early pregnancy, with an incidence of around 20%. Ultrasound scan and measurement of human chorionic gonadotropin are used to identify patients at risk of spontaneous abortion; however, there is a clinical need to find new biomarkers to prospectively identify patients before the onset of clinical symptoms. Here, we aim to investigate potential biomarkers of spontaneous abortion taken in the first clinical appointment of pregnancy. A case–control study was conducted based on a prospectively collected cohort in which cases and controls were retrospectively stratified based on pregnancy outcome: normal healthy pregnancies (controls = 33) and pregnancies that ended in spontaneous abortion (cases = 10). We evaluated extracellular vesicles isolated by precipitation with ExoQuick™ and protein concentrations of tissue plasminogen activator, leptin, and adiponectin measured by ELISA. The extracellular vesicles showed the typical morphology and membrane proteins: CD63, Alix, and Flotilin-1. The size distributions of the isolated extracellular vesicles were 112 ± 27 and 118 ± 28 nm in diameter for controls and spontaneous abortion, respectively, and the total amount of extracellular vesicles did not show any difference between controls and the spontaneous abortion group. The tissue plasminogen activator showed a significant difference (p = 0.0004) between both groups, although neither adiponectin nor leptin revealed significant changes, indicating that women who had spontaneous abortions have significantly higher levels of tissue plasminogen activator than women who had normal pregnancies.
format Online
Article
Text
id pubmed-7235768
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-72357682020-05-22 Increased Circulating Levels of Tissue-Type Plasminogen Activator Are Associated with the Risk of Spontaneous Abortion During the First Trimester of Pregnancy Monteiro, Lara J. Varas-Godoy, Manuel Acuña-Gallardo, Stephanie Correa, Paula Passalacqua, Gianluca Monckeberg, Max Rice, Gregory E. Illanes, Sebastián E. Diagnostics (Basel) Article Spontaneous abortion is a common complication in early pregnancy, with an incidence of around 20%. Ultrasound scan and measurement of human chorionic gonadotropin are used to identify patients at risk of spontaneous abortion; however, there is a clinical need to find new biomarkers to prospectively identify patients before the onset of clinical symptoms. Here, we aim to investigate potential biomarkers of spontaneous abortion taken in the first clinical appointment of pregnancy. A case–control study was conducted based on a prospectively collected cohort in which cases and controls were retrospectively stratified based on pregnancy outcome: normal healthy pregnancies (controls = 33) and pregnancies that ended in spontaneous abortion (cases = 10). We evaluated extracellular vesicles isolated by precipitation with ExoQuick™ and protein concentrations of tissue plasminogen activator, leptin, and adiponectin measured by ELISA. The extracellular vesicles showed the typical morphology and membrane proteins: CD63, Alix, and Flotilin-1. The size distributions of the isolated extracellular vesicles were 112 ± 27 and 118 ± 28 nm in diameter for controls and spontaneous abortion, respectively, and the total amount of extracellular vesicles did not show any difference between controls and the spontaneous abortion group. The tissue plasminogen activator showed a significant difference (p = 0.0004) between both groups, although neither adiponectin nor leptin revealed significant changes, indicating that women who had spontaneous abortions have significantly higher levels of tissue plasminogen activator than women who had normal pregnancies. MDPI 2020-04-01 /pmc/articles/PMC7235768/ /pubmed/32244842 http://dx.doi.org/10.3390/diagnostics10040197 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Monteiro, Lara J.
Varas-Godoy, Manuel
Acuña-Gallardo, Stephanie
Correa, Paula
Passalacqua, Gianluca
Monckeberg, Max
Rice, Gregory E.
Illanes, Sebastián E.
Increased Circulating Levels of Tissue-Type Plasminogen Activator Are Associated with the Risk of Spontaneous Abortion During the First Trimester of Pregnancy
title Increased Circulating Levels of Tissue-Type Plasminogen Activator Are Associated with the Risk of Spontaneous Abortion During the First Trimester of Pregnancy
title_full Increased Circulating Levels of Tissue-Type Plasminogen Activator Are Associated with the Risk of Spontaneous Abortion During the First Trimester of Pregnancy
title_fullStr Increased Circulating Levels of Tissue-Type Plasminogen Activator Are Associated with the Risk of Spontaneous Abortion During the First Trimester of Pregnancy
title_full_unstemmed Increased Circulating Levels of Tissue-Type Plasminogen Activator Are Associated with the Risk of Spontaneous Abortion During the First Trimester of Pregnancy
title_short Increased Circulating Levels of Tissue-Type Plasminogen Activator Are Associated with the Risk of Spontaneous Abortion During the First Trimester of Pregnancy
title_sort increased circulating levels of tissue-type plasminogen activator are associated with the risk of spontaneous abortion during the first trimester of pregnancy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7235768/
https://www.ncbi.nlm.nih.gov/pubmed/32244842
http://dx.doi.org/10.3390/diagnostics10040197
work_keys_str_mv AT monteirolaraj increasedcirculatinglevelsoftissuetypeplasminogenactivatorareassociatedwiththeriskofspontaneousabortionduringthefirsttrimesterofpregnancy
AT varasgodoymanuel increasedcirculatinglevelsoftissuetypeplasminogenactivatorareassociatedwiththeriskofspontaneousabortionduringthefirsttrimesterofpregnancy
AT acunagallardostephanie increasedcirculatinglevelsoftissuetypeplasminogenactivatorareassociatedwiththeriskofspontaneousabortionduringthefirsttrimesterofpregnancy
AT correapaula increasedcirculatinglevelsoftissuetypeplasminogenactivatorareassociatedwiththeriskofspontaneousabortionduringthefirsttrimesterofpregnancy
AT passalacquagianluca increasedcirculatinglevelsoftissuetypeplasminogenactivatorareassociatedwiththeriskofspontaneousabortionduringthefirsttrimesterofpregnancy
AT monckebergmax increasedcirculatinglevelsoftissuetypeplasminogenactivatorareassociatedwiththeriskofspontaneousabortionduringthefirsttrimesterofpregnancy
AT ricegregorye increasedcirculatinglevelsoftissuetypeplasminogenactivatorareassociatedwiththeriskofspontaneousabortionduringthefirsttrimesterofpregnancy
AT illanessebastiane increasedcirculatinglevelsoftissuetypeplasminogenactivatorareassociatedwiththeriskofspontaneousabortionduringthefirsttrimesterofpregnancy