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Effects of Antibiotics on the Intestinal Microbiota of Mice

Studies on human and mouse gastrointestinal microbiota have correlated the composition of the microbiota to a variety of diseases, as well as proved it vital to prevent colonization with resistant bacteria, a phenomenon known as colonization resistance. Antibiotics dramatically modify the gut commun...

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Autores principales: Hertz, Frederik Boetius, Budding, Andries E., van der Lugt-Degen, Malieka, Savelkoul, Paul H., Løbner-Olesen, Anders, Frimodt-Møller, Niels
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7235770/
https://www.ncbi.nlm.nih.gov/pubmed/32316518
http://dx.doi.org/10.3390/antibiotics9040191
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author Hertz, Frederik Boetius
Budding, Andries E.
van der Lugt-Degen, Malieka
Savelkoul, Paul H.
Løbner-Olesen, Anders
Frimodt-Møller, Niels
author_facet Hertz, Frederik Boetius
Budding, Andries E.
van der Lugt-Degen, Malieka
Savelkoul, Paul H.
Løbner-Olesen, Anders
Frimodt-Møller, Niels
author_sort Hertz, Frederik Boetius
collection PubMed
description Studies on human and mouse gastrointestinal microbiota have correlated the composition of the microbiota to a variety of diseases, as well as proved it vital to prevent colonization with resistant bacteria, a phenomenon known as colonization resistance. Antibiotics dramatically modify the gut community and there are examples of how antibiotic usage lead to colonization with resistant bacteria [e.g., dicloxacillin usage selecting for ESBL-producing E. coli carriage], as shown by Hertz et al. Here, we investigated the impact of five antibiotics [cefotaxime, cefuroxime, dicloxacillin, clindamycin, and ciprofloxacin] on the intestinal microbiota in mice. Five different antibiotics were each given to groups of five mice. The intestinal microbiotas were profiled by use of the IS-pro analysis; a 16S–23S rDNA interspace [IS]-region-based profiling method. For the mice receiving dicloxacillin and clindamycin, we observed dramatic shifts in dominating phyla from day 1 to day 5. Of note, diversity increased, but overall bacterial load decreased. For ciprofloxacin, cefotaxime, and cefuroxime there were few overall changes. We speculate that antibiotics with efficacy against the abundant anaerobes in the gut, particularly Bacteroidetes, can in fact be selected for resistant bacteria, disregarding the spectrum of activity.
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spelling pubmed-72357702020-05-22 Effects of Antibiotics on the Intestinal Microbiota of Mice Hertz, Frederik Boetius Budding, Andries E. van der Lugt-Degen, Malieka Savelkoul, Paul H. Løbner-Olesen, Anders Frimodt-Møller, Niels Antibiotics (Basel) Article Studies on human and mouse gastrointestinal microbiota have correlated the composition of the microbiota to a variety of diseases, as well as proved it vital to prevent colonization with resistant bacteria, a phenomenon known as colonization resistance. Antibiotics dramatically modify the gut community and there are examples of how antibiotic usage lead to colonization with resistant bacteria [e.g., dicloxacillin usage selecting for ESBL-producing E. coli carriage], as shown by Hertz et al. Here, we investigated the impact of five antibiotics [cefotaxime, cefuroxime, dicloxacillin, clindamycin, and ciprofloxacin] on the intestinal microbiota in mice. Five different antibiotics were each given to groups of five mice. The intestinal microbiotas were profiled by use of the IS-pro analysis; a 16S–23S rDNA interspace [IS]-region-based profiling method. For the mice receiving dicloxacillin and clindamycin, we observed dramatic shifts in dominating phyla from day 1 to day 5. Of note, diversity increased, but overall bacterial load decreased. For ciprofloxacin, cefotaxime, and cefuroxime there were few overall changes. We speculate that antibiotics with efficacy against the abundant anaerobes in the gut, particularly Bacteroidetes, can in fact be selected for resistant bacteria, disregarding the spectrum of activity. MDPI 2020-04-17 /pmc/articles/PMC7235770/ /pubmed/32316518 http://dx.doi.org/10.3390/antibiotics9040191 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hertz, Frederik Boetius
Budding, Andries E.
van der Lugt-Degen, Malieka
Savelkoul, Paul H.
Løbner-Olesen, Anders
Frimodt-Møller, Niels
Effects of Antibiotics on the Intestinal Microbiota of Mice
title Effects of Antibiotics on the Intestinal Microbiota of Mice
title_full Effects of Antibiotics on the Intestinal Microbiota of Mice
title_fullStr Effects of Antibiotics on the Intestinal Microbiota of Mice
title_full_unstemmed Effects of Antibiotics on the Intestinal Microbiota of Mice
title_short Effects of Antibiotics on the Intestinal Microbiota of Mice
title_sort effects of antibiotics on the intestinal microbiota of mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7235770/
https://www.ncbi.nlm.nih.gov/pubmed/32316518
http://dx.doi.org/10.3390/antibiotics9040191
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