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Flexible and Printed Electrochemical Immunosensor Coated with Oxygen Plasma Treated SWCNTs for Histamine Detection

Heterocyclic amine histamine is a well-known foodborne toxicant (mostly linked to “scombroid poisoning”) synthesized from the microbial decarboxylation of amino acid histidine. In this work, we report the fabrication of a flexible screen-printed immunosensor based on a silver electrode coated with s...

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Autores principales: Shkodra, Bajramshahe, Demelash Abera, Biresaw, Cantarella, Giuseppe, Douaki, Ali, Avancini, Enrico, Petti, Luisa, Lugli, Paolo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7235776/
https://www.ncbi.nlm.nih.gov/pubmed/32290233
http://dx.doi.org/10.3390/bios10040035
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author Shkodra, Bajramshahe
Demelash Abera, Biresaw
Cantarella, Giuseppe
Douaki, Ali
Avancini, Enrico
Petti, Luisa
Lugli, Paolo
author_facet Shkodra, Bajramshahe
Demelash Abera, Biresaw
Cantarella, Giuseppe
Douaki, Ali
Avancini, Enrico
Petti, Luisa
Lugli, Paolo
author_sort Shkodra, Bajramshahe
collection PubMed
description Heterocyclic amine histamine is a well-known foodborne toxicant (mostly linked to “scombroid poisoning”) synthesized from the microbial decarboxylation of amino acid histidine. In this work, we report the fabrication of a flexible screen-printed immunosensor based on a silver electrode coated with single-walled carbon nanotubes (SWCNTs) for the detection of histamine directly in fish samples. Biosensors were realized by first spray depositing SWCNTs on the working electrodes and by subsequently treating them with oxygen plasma to reduce the unwanted effects related to their hydrophobicity. Next, anti-histamine antibodies were directly immobilized on the treated SWCNTs. Histamine was detected using the typical reaction of histamine and histamine-labeled with horseradish peroxidase (HRP) competing to bind with anti-histamine antibodies. The developed immunosensor shows a wide linear detection range from 0.005 to 50 ng/mL for histamine samples, with a coefficient of determination as high as 98.05%. Average recoveries in fish samples were observed from 96.00% to 104.7%. The biosensor also shows good selectivity (less than 3% relative response for cadaverine, putrescine, and tyramine), reproducibility, mechanical and time stability, being a promising analytical tool for the analysis of histamine, as well as of other food hazards.
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spelling pubmed-72357762020-05-22 Flexible and Printed Electrochemical Immunosensor Coated with Oxygen Plasma Treated SWCNTs for Histamine Detection Shkodra, Bajramshahe Demelash Abera, Biresaw Cantarella, Giuseppe Douaki, Ali Avancini, Enrico Petti, Luisa Lugli, Paolo Biosensors (Basel) Article Heterocyclic amine histamine is a well-known foodborne toxicant (mostly linked to “scombroid poisoning”) synthesized from the microbial decarboxylation of amino acid histidine. In this work, we report the fabrication of a flexible screen-printed immunosensor based on a silver electrode coated with single-walled carbon nanotubes (SWCNTs) for the detection of histamine directly in fish samples. Biosensors were realized by first spray depositing SWCNTs on the working electrodes and by subsequently treating them with oxygen plasma to reduce the unwanted effects related to their hydrophobicity. Next, anti-histamine antibodies were directly immobilized on the treated SWCNTs. Histamine was detected using the typical reaction of histamine and histamine-labeled with horseradish peroxidase (HRP) competing to bind with anti-histamine antibodies. The developed immunosensor shows a wide linear detection range from 0.005 to 50 ng/mL for histamine samples, with a coefficient of determination as high as 98.05%. Average recoveries in fish samples were observed from 96.00% to 104.7%. The biosensor also shows good selectivity (less than 3% relative response for cadaverine, putrescine, and tyramine), reproducibility, mechanical and time stability, being a promising analytical tool for the analysis of histamine, as well as of other food hazards. MDPI 2020-04-10 /pmc/articles/PMC7235776/ /pubmed/32290233 http://dx.doi.org/10.3390/bios10040035 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Shkodra, Bajramshahe
Demelash Abera, Biresaw
Cantarella, Giuseppe
Douaki, Ali
Avancini, Enrico
Petti, Luisa
Lugli, Paolo
Flexible and Printed Electrochemical Immunosensor Coated with Oxygen Plasma Treated SWCNTs for Histamine Detection
title Flexible and Printed Electrochemical Immunosensor Coated with Oxygen Plasma Treated SWCNTs for Histamine Detection
title_full Flexible and Printed Electrochemical Immunosensor Coated with Oxygen Plasma Treated SWCNTs for Histamine Detection
title_fullStr Flexible and Printed Electrochemical Immunosensor Coated with Oxygen Plasma Treated SWCNTs for Histamine Detection
title_full_unstemmed Flexible and Printed Electrochemical Immunosensor Coated with Oxygen Plasma Treated SWCNTs for Histamine Detection
title_short Flexible and Printed Electrochemical Immunosensor Coated with Oxygen Plasma Treated SWCNTs for Histamine Detection
title_sort flexible and printed electrochemical immunosensor coated with oxygen plasma treated swcnts for histamine detection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7235776/
https://www.ncbi.nlm.nih.gov/pubmed/32290233
http://dx.doi.org/10.3390/bios10040035
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