Cargando…

Assessment of the Usefulness of Cefapirin and Cefalonium Disks for Susceptibility Testing of Staphylococcus aureus Isolates from Bovine Mastitis

Cefapirin (CEP) and cefalonium (CNM) are first-generation cephalosporins widely used to treat bovine mastitis caused by Gram-positive bacteria including staphylococci. However, disks for susceptibility testing of those drugs in causative bacteria are not available. This study evaluated the efficacy...

Descripción completa

Detalles Bibliográficos
Autores principales: Harada, Kazuki, Irie, Shieri, Ohnishi, Mamoru, Kataoka, Yasushi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7235829/
https://www.ncbi.nlm.nih.gov/pubmed/32326215
http://dx.doi.org/10.3390/antibiotics9040197
_version_ 1783536045388726272
author Harada, Kazuki
Irie, Shieri
Ohnishi, Mamoru
Kataoka, Yasushi
author_facet Harada, Kazuki
Irie, Shieri
Ohnishi, Mamoru
Kataoka, Yasushi
author_sort Harada, Kazuki
collection PubMed
description Cefapirin (CEP) and cefalonium (CNM) are first-generation cephalosporins widely used to treat bovine mastitis caused by Gram-positive bacteria including staphylococci. However, disks for susceptibility testing of those drugs in causative bacteria are not available. This study evaluated the efficacy of 10 µg and 30 µg pilot disks of CEP (CEP10 and CEP30) and CNM (CNM10 and CNM30) against 130 Staphylococcus aureus isolates from bovine mastitis. Scattergrams of minimum inhibitory concentrations (MICs) and zone diameters (ZDs) illustrated significant correlations between the MICs and ZDs of CEP10 (r = −0.912), CEP30 (r = −0.933), CNM10 (r = −0.847), and CNM30 (r = −0.807). The analysis by Normalized Resistance Interpretation indicated that the epidemiolocal cut-off value (ECV) of MIC for both cefapirin and cefalonium is ≤ 0.5 µg/mL, and the ECV of ZD for CEP10, CEP30, CNM10, and CNM30 were ≥ 22 mm, ≥ 25 mm, ≥ 22 mm, and ≥ 29 mm, respectively. We believe that both 10 μg and 30 μg CEP and CNM susceptibility disks will be helpful for guiding the appropriate use of these antibiotics for bovine mastitis. Further studies toward the establishment of clinical breakpoint of CEP and CNM would be needed for their routine use.
format Online
Article
Text
id pubmed-7235829
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-72358292020-05-22 Assessment of the Usefulness of Cefapirin and Cefalonium Disks for Susceptibility Testing of Staphylococcus aureus Isolates from Bovine Mastitis Harada, Kazuki Irie, Shieri Ohnishi, Mamoru Kataoka, Yasushi Antibiotics (Basel) Article Cefapirin (CEP) and cefalonium (CNM) are first-generation cephalosporins widely used to treat bovine mastitis caused by Gram-positive bacteria including staphylococci. However, disks for susceptibility testing of those drugs in causative bacteria are not available. This study evaluated the efficacy of 10 µg and 30 µg pilot disks of CEP (CEP10 and CEP30) and CNM (CNM10 and CNM30) against 130 Staphylococcus aureus isolates from bovine mastitis. Scattergrams of minimum inhibitory concentrations (MICs) and zone diameters (ZDs) illustrated significant correlations between the MICs and ZDs of CEP10 (r = −0.912), CEP30 (r = −0.933), CNM10 (r = −0.847), and CNM30 (r = −0.807). The analysis by Normalized Resistance Interpretation indicated that the epidemiolocal cut-off value (ECV) of MIC for both cefapirin and cefalonium is ≤ 0.5 µg/mL, and the ECV of ZD for CEP10, CEP30, CNM10, and CNM30 were ≥ 22 mm, ≥ 25 mm, ≥ 22 mm, and ≥ 29 mm, respectively. We believe that both 10 μg and 30 μg CEP and CNM susceptibility disks will be helpful for guiding the appropriate use of these antibiotics for bovine mastitis. Further studies toward the establishment of clinical breakpoint of CEP and CNM would be needed for their routine use. MDPI 2020-04-21 /pmc/articles/PMC7235829/ /pubmed/32326215 http://dx.doi.org/10.3390/antibiotics9040197 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Harada, Kazuki
Irie, Shieri
Ohnishi, Mamoru
Kataoka, Yasushi
Assessment of the Usefulness of Cefapirin and Cefalonium Disks for Susceptibility Testing of Staphylococcus aureus Isolates from Bovine Mastitis
title Assessment of the Usefulness of Cefapirin and Cefalonium Disks for Susceptibility Testing of Staphylococcus aureus Isolates from Bovine Mastitis
title_full Assessment of the Usefulness of Cefapirin and Cefalonium Disks for Susceptibility Testing of Staphylococcus aureus Isolates from Bovine Mastitis
title_fullStr Assessment of the Usefulness of Cefapirin and Cefalonium Disks for Susceptibility Testing of Staphylococcus aureus Isolates from Bovine Mastitis
title_full_unstemmed Assessment of the Usefulness of Cefapirin and Cefalonium Disks for Susceptibility Testing of Staphylococcus aureus Isolates from Bovine Mastitis
title_short Assessment of the Usefulness of Cefapirin and Cefalonium Disks for Susceptibility Testing of Staphylococcus aureus Isolates from Bovine Mastitis
title_sort assessment of the usefulness of cefapirin and cefalonium disks for susceptibility testing of staphylococcus aureus isolates from bovine mastitis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7235829/
https://www.ncbi.nlm.nih.gov/pubmed/32326215
http://dx.doi.org/10.3390/antibiotics9040197
work_keys_str_mv AT haradakazuki assessmentoftheusefulnessofcefapirinandcefaloniumdisksforsusceptibilitytestingofstaphylococcusaureusisolatesfrombovinemastitis
AT irieshieri assessmentoftheusefulnessofcefapirinandcefaloniumdisksforsusceptibilitytestingofstaphylococcusaureusisolatesfrombovinemastitis
AT ohnishimamoru assessmentoftheusefulnessofcefapirinandcefaloniumdisksforsusceptibilitytestingofstaphylococcusaureusisolatesfrombovinemastitis
AT kataokayasushi assessmentoftheusefulnessofcefapirinandcefaloniumdisksforsusceptibilitytestingofstaphylococcusaureusisolatesfrombovinemastitis