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Characterization of the Antinociceptive Activity from Stevia serrata Cav

Background: Stevia serrata Cav. (Asteraceae), widely found in Guatemala, is used to treat gastrointestinal problems. The aim of this study was to demonstrate the antinociceptive and anti-inflammatory effects of the essential oil (EO) and the mechanism of action. Methods: EO was tested in chemical (c...

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Autores principales: Cordeiro, Millena S., Simas, Daniel L. R., Pérez-Sabino, Juan F., Mérida-Reyes, Max S., Muñoz-Wug, Manuel A., Oliva-Hernández, Bessie E., da Silva, Antônio J. R., Fernandes, Patricia D., Giorno, Thais B. S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7235832/
https://www.ncbi.nlm.nih.gov/pubmed/32272558
http://dx.doi.org/10.3390/biomedicines8040079
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author Cordeiro, Millena S.
Simas, Daniel L. R.
Pérez-Sabino, Juan F.
Mérida-Reyes, Max S.
Muñoz-Wug, Manuel A.
Oliva-Hernández, Bessie E.
da Silva, Antônio J. R.
Fernandes, Patricia D.
Giorno, Thais B. S.
author_facet Cordeiro, Millena S.
Simas, Daniel L. R.
Pérez-Sabino, Juan F.
Mérida-Reyes, Max S.
Muñoz-Wug, Manuel A.
Oliva-Hernández, Bessie E.
da Silva, Antônio J. R.
Fernandes, Patricia D.
Giorno, Thais B. S.
author_sort Cordeiro, Millena S.
collection PubMed
description Background: Stevia serrata Cav. (Asteraceae), widely found in Guatemala, is used to treat gastrointestinal problems. The aim of this study was to demonstrate the antinociceptive and anti-inflammatory effects of the essential oil (EO) and the mechanism of action. Methods: EO was tested in chemical (capsaicin- and glutamate-induced licking response) or thermal (hot plate) models of nociception at 10, 30 or 100 mg/kg doses. The mechanism of action was evaluated using two receptor antagonists (naloxone, atropine) and an enzyme inhibitor (L-NAME). The anti-hyperalgesic effect was evaluated using carrageenan-induced nociception and evaluated in the hot plate. Results: All three doses of EO reduced licking response induced by glutamate, and higher doses reduced capsaicin-induced licking. EO also increased area under the curve, similar to the morphine-treated group. The antinociceptive effect induced by EO was reversed by pretreatment of mice with naloxone (1 mg/kg, ip), atropine (1 mg/kg, ip) or L-NAME (3 mg/kg, ip). EO also demonstrated an anti-hyperalgesic effect. The 100 mg/kg dose increased the latency time, even at 1 h after oral administration and this effect has been maintained until the 96th hour, post-administration. Conclusions: Our data suggest that essential oil of S. serrata presents an antinociceptive effect mediated, at least in part, through activation of opioid, cholinergic and nitrergic pathways.
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spelling pubmed-72358322020-05-22 Characterization of the Antinociceptive Activity from Stevia serrata Cav Cordeiro, Millena S. Simas, Daniel L. R. Pérez-Sabino, Juan F. Mérida-Reyes, Max S. Muñoz-Wug, Manuel A. Oliva-Hernández, Bessie E. da Silva, Antônio J. R. Fernandes, Patricia D. Giorno, Thais B. S. Biomedicines Article Background: Stevia serrata Cav. (Asteraceae), widely found in Guatemala, is used to treat gastrointestinal problems. The aim of this study was to demonstrate the antinociceptive and anti-inflammatory effects of the essential oil (EO) and the mechanism of action. Methods: EO was tested in chemical (capsaicin- and glutamate-induced licking response) or thermal (hot plate) models of nociception at 10, 30 or 100 mg/kg doses. The mechanism of action was evaluated using two receptor antagonists (naloxone, atropine) and an enzyme inhibitor (L-NAME). The anti-hyperalgesic effect was evaluated using carrageenan-induced nociception and evaluated in the hot plate. Results: All three doses of EO reduced licking response induced by glutamate, and higher doses reduced capsaicin-induced licking. EO also increased area under the curve, similar to the morphine-treated group. The antinociceptive effect induced by EO was reversed by pretreatment of mice with naloxone (1 mg/kg, ip), atropine (1 mg/kg, ip) or L-NAME (3 mg/kg, ip). EO also demonstrated an anti-hyperalgesic effect. The 100 mg/kg dose increased the latency time, even at 1 h after oral administration and this effect has been maintained until the 96th hour, post-administration. Conclusions: Our data suggest that essential oil of S. serrata presents an antinociceptive effect mediated, at least in part, through activation of opioid, cholinergic and nitrergic pathways. MDPI 2020-04-07 /pmc/articles/PMC7235832/ /pubmed/32272558 http://dx.doi.org/10.3390/biomedicines8040079 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Cordeiro, Millena S.
Simas, Daniel L. R.
Pérez-Sabino, Juan F.
Mérida-Reyes, Max S.
Muñoz-Wug, Manuel A.
Oliva-Hernández, Bessie E.
da Silva, Antônio J. R.
Fernandes, Patricia D.
Giorno, Thais B. S.
Characterization of the Antinociceptive Activity from Stevia serrata Cav
title Characterization of the Antinociceptive Activity from Stevia serrata Cav
title_full Characterization of the Antinociceptive Activity from Stevia serrata Cav
title_fullStr Characterization of the Antinociceptive Activity from Stevia serrata Cav
title_full_unstemmed Characterization of the Antinociceptive Activity from Stevia serrata Cav
title_short Characterization of the Antinociceptive Activity from Stevia serrata Cav
title_sort characterization of the antinociceptive activity from stevia serrata cav
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7235832/
https://www.ncbi.nlm.nih.gov/pubmed/32272558
http://dx.doi.org/10.3390/biomedicines8040079
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