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Characterization of the Antinociceptive Activity from Stevia serrata Cav
Background: Stevia serrata Cav. (Asteraceae), widely found in Guatemala, is used to treat gastrointestinal problems. The aim of this study was to demonstrate the antinociceptive and anti-inflammatory effects of the essential oil (EO) and the mechanism of action. Methods: EO was tested in chemical (c...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7235832/ https://www.ncbi.nlm.nih.gov/pubmed/32272558 http://dx.doi.org/10.3390/biomedicines8040079 |
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author | Cordeiro, Millena S. Simas, Daniel L. R. Pérez-Sabino, Juan F. Mérida-Reyes, Max S. Muñoz-Wug, Manuel A. Oliva-Hernández, Bessie E. da Silva, Antônio J. R. Fernandes, Patricia D. Giorno, Thais B. S. |
author_facet | Cordeiro, Millena S. Simas, Daniel L. R. Pérez-Sabino, Juan F. Mérida-Reyes, Max S. Muñoz-Wug, Manuel A. Oliva-Hernández, Bessie E. da Silva, Antônio J. R. Fernandes, Patricia D. Giorno, Thais B. S. |
author_sort | Cordeiro, Millena S. |
collection | PubMed |
description | Background: Stevia serrata Cav. (Asteraceae), widely found in Guatemala, is used to treat gastrointestinal problems. The aim of this study was to demonstrate the antinociceptive and anti-inflammatory effects of the essential oil (EO) and the mechanism of action. Methods: EO was tested in chemical (capsaicin- and glutamate-induced licking response) or thermal (hot plate) models of nociception at 10, 30 or 100 mg/kg doses. The mechanism of action was evaluated using two receptor antagonists (naloxone, atropine) and an enzyme inhibitor (L-NAME). The anti-hyperalgesic effect was evaluated using carrageenan-induced nociception and evaluated in the hot plate. Results: All three doses of EO reduced licking response induced by glutamate, and higher doses reduced capsaicin-induced licking. EO also increased area under the curve, similar to the morphine-treated group. The antinociceptive effect induced by EO was reversed by pretreatment of mice with naloxone (1 mg/kg, ip), atropine (1 mg/kg, ip) or L-NAME (3 mg/kg, ip). EO also demonstrated an anti-hyperalgesic effect. The 100 mg/kg dose increased the latency time, even at 1 h after oral administration and this effect has been maintained until the 96th hour, post-administration. Conclusions: Our data suggest that essential oil of S. serrata presents an antinociceptive effect mediated, at least in part, through activation of opioid, cholinergic and nitrergic pathways. |
format | Online Article Text |
id | pubmed-7235832 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-72358322020-05-22 Characterization of the Antinociceptive Activity from Stevia serrata Cav Cordeiro, Millena S. Simas, Daniel L. R. Pérez-Sabino, Juan F. Mérida-Reyes, Max S. Muñoz-Wug, Manuel A. Oliva-Hernández, Bessie E. da Silva, Antônio J. R. Fernandes, Patricia D. Giorno, Thais B. S. Biomedicines Article Background: Stevia serrata Cav. (Asteraceae), widely found in Guatemala, is used to treat gastrointestinal problems. The aim of this study was to demonstrate the antinociceptive and anti-inflammatory effects of the essential oil (EO) and the mechanism of action. Methods: EO was tested in chemical (capsaicin- and glutamate-induced licking response) or thermal (hot plate) models of nociception at 10, 30 or 100 mg/kg doses. The mechanism of action was evaluated using two receptor antagonists (naloxone, atropine) and an enzyme inhibitor (L-NAME). The anti-hyperalgesic effect was evaluated using carrageenan-induced nociception and evaluated in the hot plate. Results: All three doses of EO reduced licking response induced by glutamate, and higher doses reduced capsaicin-induced licking. EO also increased area under the curve, similar to the morphine-treated group. The antinociceptive effect induced by EO was reversed by pretreatment of mice with naloxone (1 mg/kg, ip), atropine (1 mg/kg, ip) or L-NAME (3 mg/kg, ip). EO also demonstrated an anti-hyperalgesic effect. The 100 mg/kg dose increased the latency time, even at 1 h after oral administration and this effect has been maintained until the 96th hour, post-administration. Conclusions: Our data suggest that essential oil of S. serrata presents an antinociceptive effect mediated, at least in part, through activation of opioid, cholinergic and nitrergic pathways. MDPI 2020-04-07 /pmc/articles/PMC7235832/ /pubmed/32272558 http://dx.doi.org/10.3390/biomedicines8040079 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Cordeiro, Millena S. Simas, Daniel L. R. Pérez-Sabino, Juan F. Mérida-Reyes, Max S. Muñoz-Wug, Manuel A. Oliva-Hernández, Bessie E. da Silva, Antônio J. R. Fernandes, Patricia D. Giorno, Thais B. S. Characterization of the Antinociceptive Activity from Stevia serrata Cav |
title | Characterization of the Antinociceptive Activity from Stevia serrata Cav |
title_full | Characterization of the Antinociceptive Activity from Stevia serrata Cav |
title_fullStr | Characterization of the Antinociceptive Activity from Stevia serrata Cav |
title_full_unstemmed | Characterization of the Antinociceptive Activity from Stevia serrata Cav |
title_short | Characterization of the Antinociceptive Activity from Stevia serrata Cav |
title_sort | characterization of the antinociceptive activity from stevia serrata cav |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7235832/ https://www.ncbi.nlm.nih.gov/pubmed/32272558 http://dx.doi.org/10.3390/biomedicines8040079 |
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