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Performance of malaria microscopy external quality assessment and networking among health facilities in west Amhara region, Ethiopia
BACKGROUND: Microscopic examination of peripheral blood smear produces reliable results both about the malaria infection status and level of parasitemia. However, test results are affected by skill of the laboratory personnel, workload, condition of microscopes and quality of laboratory supplies. Th...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7236141/ https://www.ncbi.nlm.nih.gov/pubmed/32429860 http://dx.doi.org/10.1186/s12879-020-05077-5 |
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author | Tegegne, Banchamlak Ejigu, Kefale Alemu, Getaneh Fetene, Yeshimebet Endaylalu, Kindye Melese, Mulatu |
author_facet | Tegegne, Banchamlak Ejigu, Kefale Alemu, Getaneh Fetene, Yeshimebet Endaylalu, Kindye Melese, Mulatu |
author_sort | Tegegne, Banchamlak |
collection | PubMed |
description | BACKGROUND: Microscopic examination of peripheral blood smear produces reliable results both about the malaria infection status and level of parasitemia. However, test results are affected by skill of the laboratory personnel, workload, condition of microscopes and quality of laboratory supplies. Therefore, continuous monitoring of the performance of laboratories is of pivotal importance in order to make timely correction. METHODS: A facility based cross-sectional study was conducted from July 2017 to July 2019 to assess malaria microscopy performance among thirty malaria diagnostic laboratories in west Amhara region. Thirty slides were collected from participating laboratories every quarter. Collected slides were taken to Amhara Public Health Institute reference laboratory and re-checked by malaria microscopists who were blind to the results from health facilities. Percentage of test agreement, rates of false positive, false negative and species misdiagnosis were calculated using Excel 2010. RESULTS: Among a total of 6689 slides re-checked, results of 6146 slides were the same with that of participating laboratories. The test agreement was 97.31 and 94.6% for parasite detection and species identification, respectively. Variations in the overall performance of individual laboratories were seen within a range of 81.55 to 97.27% test agreement. Results of 543 (8.12%) slides were discordant, of which 363 (5.4%), 93 (1.4%) and 87 (1.3%) slides were due to species misdiagnosis, false positive and false negative results, respectively. CONCLUSION: There was good test agreement between participated laboratories and Amhara Public Health Institute. More accurate performance is expected as the country is tracking to malaria elimination. Hence, further strengthening the external quality assurance program is recommended. |
format | Online Article Text |
id | pubmed-7236141 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-72361412020-05-27 Performance of malaria microscopy external quality assessment and networking among health facilities in west Amhara region, Ethiopia Tegegne, Banchamlak Ejigu, Kefale Alemu, Getaneh Fetene, Yeshimebet Endaylalu, Kindye Melese, Mulatu BMC Infect Dis Research Article BACKGROUND: Microscopic examination of peripheral blood smear produces reliable results both about the malaria infection status and level of parasitemia. However, test results are affected by skill of the laboratory personnel, workload, condition of microscopes and quality of laboratory supplies. Therefore, continuous monitoring of the performance of laboratories is of pivotal importance in order to make timely correction. METHODS: A facility based cross-sectional study was conducted from July 2017 to July 2019 to assess malaria microscopy performance among thirty malaria diagnostic laboratories in west Amhara region. Thirty slides were collected from participating laboratories every quarter. Collected slides were taken to Amhara Public Health Institute reference laboratory and re-checked by malaria microscopists who were blind to the results from health facilities. Percentage of test agreement, rates of false positive, false negative and species misdiagnosis were calculated using Excel 2010. RESULTS: Among a total of 6689 slides re-checked, results of 6146 slides were the same with that of participating laboratories. The test agreement was 97.31 and 94.6% for parasite detection and species identification, respectively. Variations in the overall performance of individual laboratories were seen within a range of 81.55 to 97.27% test agreement. Results of 543 (8.12%) slides were discordant, of which 363 (5.4%), 93 (1.4%) and 87 (1.3%) slides were due to species misdiagnosis, false positive and false negative results, respectively. CONCLUSION: There was good test agreement between participated laboratories and Amhara Public Health Institute. More accurate performance is expected as the country is tracking to malaria elimination. Hence, further strengthening the external quality assurance program is recommended. BioMed Central 2020-05-19 /pmc/articles/PMC7236141/ /pubmed/32429860 http://dx.doi.org/10.1186/s12879-020-05077-5 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Article Tegegne, Banchamlak Ejigu, Kefale Alemu, Getaneh Fetene, Yeshimebet Endaylalu, Kindye Melese, Mulatu Performance of malaria microscopy external quality assessment and networking among health facilities in west Amhara region, Ethiopia |
title | Performance of malaria microscopy external quality assessment and networking among health facilities in west Amhara region, Ethiopia |
title_full | Performance of malaria microscopy external quality assessment and networking among health facilities in west Amhara region, Ethiopia |
title_fullStr | Performance of malaria microscopy external quality assessment and networking among health facilities in west Amhara region, Ethiopia |
title_full_unstemmed | Performance of malaria microscopy external quality assessment and networking among health facilities in west Amhara region, Ethiopia |
title_short | Performance of malaria microscopy external quality assessment and networking among health facilities in west Amhara region, Ethiopia |
title_sort | performance of malaria microscopy external quality assessment and networking among health facilities in west amhara region, ethiopia |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7236141/ https://www.ncbi.nlm.nih.gov/pubmed/32429860 http://dx.doi.org/10.1186/s12879-020-05077-5 |
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