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Performance of malaria microscopy external quality assessment and networking among health facilities in west Amhara region, Ethiopia

BACKGROUND: Microscopic examination of peripheral blood smear produces reliable results both about the malaria infection status and level of parasitemia. However, test results are affected by skill of the laboratory personnel, workload, condition of microscopes and quality of laboratory supplies. Th...

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Autores principales: Tegegne, Banchamlak, Ejigu, Kefale, Alemu, Getaneh, Fetene, Yeshimebet, Endaylalu, Kindye, Melese, Mulatu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7236141/
https://www.ncbi.nlm.nih.gov/pubmed/32429860
http://dx.doi.org/10.1186/s12879-020-05077-5
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author Tegegne, Banchamlak
Ejigu, Kefale
Alemu, Getaneh
Fetene, Yeshimebet
Endaylalu, Kindye
Melese, Mulatu
author_facet Tegegne, Banchamlak
Ejigu, Kefale
Alemu, Getaneh
Fetene, Yeshimebet
Endaylalu, Kindye
Melese, Mulatu
author_sort Tegegne, Banchamlak
collection PubMed
description BACKGROUND: Microscopic examination of peripheral blood smear produces reliable results both about the malaria infection status and level of parasitemia. However, test results are affected by skill of the laboratory personnel, workload, condition of microscopes and quality of laboratory supplies. Therefore, continuous monitoring of the performance of laboratories is of pivotal importance in order to make timely correction. METHODS: A facility based cross-sectional study was conducted from July 2017 to July 2019 to assess malaria microscopy performance among thirty malaria diagnostic laboratories in west Amhara region. Thirty slides were collected from participating laboratories every quarter. Collected slides were taken to Amhara Public Health Institute reference laboratory and re-checked by malaria microscopists who were blind to the results from health facilities. Percentage of test agreement, rates of false positive, false negative and species misdiagnosis were calculated using Excel 2010. RESULTS: Among a total of 6689 slides re-checked, results of 6146 slides were the same with that of participating laboratories. The test agreement was 97.31 and 94.6% for parasite detection and species identification, respectively. Variations in the overall performance of individual laboratories were seen within a range of 81.55 to 97.27% test agreement. Results of 543 (8.12%) slides were discordant, of which 363 (5.4%), 93 (1.4%) and 87 (1.3%) slides were due to species misdiagnosis, false positive and false negative results, respectively. CONCLUSION: There was good test agreement between participated laboratories and Amhara Public Health Institute. More accurate performance is expected as the country is tracking to malaria elimination. Hence, further strengthening the external quality assurance program is recommended.
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spelling pubmed-72361412020-05-27 Performance of malaria microscopy external quality assessment and networking among health facilities in west Amhara region, Ethiopia Tegegne, Banchamlak Ejigu, Kefale Alemu, Getaneh Fetene, Yeshimebet Endaylalu, Kindye Melese, Mulatu BMC Infect Dis Research Article BACKGROUND: Microscopic examination of peripheral blood smear produces reliable results both about the malaria infection status and level of parasitemia. However, test results are affected by skill of the laboratory personnel, workload, condition of microscopes and quality of laboratory supplies. Therefore, continuous monitoring of the performance of laboratories is of pivotal importance in order to make timely correction. METHODS: A facility based cross-sectional study was conducted from July 2017 to July 2019 to assess malaria microscopy performance among thirty malaria diagnostic laboratories in west Amhara region. Thirty slides were collected from participating laboratories every quarter. Collected slides were taken to Amhara Public Health Institute reference laboratory and re-checked by malaria microscopists who were blind to the results from health facilities. Percentage of test agreement, rates of false positive, false negative and species misdiagnosis were calculated using Excel 2010. RESULTS: Among a total of 6689 slides re-checked, results of 6146 slides were the same with that of participating laboratories. The test agreement was 97.31 and 94.6% for parasite detection and species identification, respectively. Variations in the overall performance of individual laboratories were seen within a range of 81.55 to 97.27% test agreement. Results of 543 (8.12%) slides were discordant, of which 363 (5.4%), 93 (1.4%) and 87 (1.3%) slides were due to species misdiagnosis, false positive and false negative results, respectively. CONCLUSION: There was good test agreement between participated laboratories and Amhara Public Health Institute. More accurate performance is expected as the country is tracking to malaria elimination. Hence, further strengthening the external quality assurance program is recommended. BioMed Central 2020-05-19 /pmc/articles/PMC7236141/ /pubmed/32429860 http://dx.doi.org/10.1186/s12879-020-05077-5 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
Tegegne, Banchamlak
Ejigu, Kefale
Alemu, Getaneh
Fetene, Yeshimebet
Endaylalu, Kindye
Melese, Mulatu
Performance of malaria microscopy external quality assessment and networking among health facilities in west Amhara region, Ethiopia
title Performance of malaria microscopy external quality assessment and networking among health facilities in west Amhara region, Ethiopia
title_full Performance of malaria microscopy external quality assessment and networking among health facilities in west Amhara region, Ethiopia
title_fullStr Performance of malaria microscopy external quality assessment and networking among health facilities in west Amhara region, Ethiopia
title_full_unstemmed Performance of malaria microscopy external quality assessment and networking among health facilities in west Amhara region, Ethiopia
title_short Performance of malaria microscopy external quality assessment and networking among health facilities in west Amhara region, Ethiopia
title_sort performance of malaria microscopy external quality assessment and networking among health facilities in west amhara region, ethiopia
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7236141/
https://www.ncbi.nlm.nih.gov/pubmed/32429860
http://dx.doi.org/10.1186/s12879-020-05077-5
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