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Effects of various inhibitory substances and immobilization on ethanol production efficiency of a thermotolerant Pichia kudriavzevii

BACKGROUND: Although bioethanol production has been gaining worldwide attention as an alternative to fossil fuel, ethanol productivities and yields are still limited due to the susceptibility of fermentation microorganisms to various stress and inhibitory substances. There is therefore an unmet need...

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Autores principales: Ndubuisi, Ifeanyi A., Qin, Qijian, Liao, Guiyan, Wang, Bin, Moneke, Anene N., Ogbonna, James C., Jin, Cheng, Fang, Wenxia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7236494/
https://www.ncbi.nlm.nih.gov/pubmed/32477425
http://dx.doi.org/10.1186/s13068-020-01729-5
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author Ndubuisi, Ifeanyi A.
Qin, Qijian
Liao, Guiyan
Wang, Bin
Moneke, Anene N.
Ogbonna, James C.
Jin, Cheng
Fang, Wenxia
author_facet Ndubuisi, Ifeanyi A.
Qin, Qijian
Liao, Guiyan
Wang, Bin
Moneke, Anene N.
Ogbonna, James C.
Jin, Cheng
Fang, Wenxia
author_sort Ndubuisi, Ifeanyi A.
collection PubMed
description BACKGROUND: Although bioethanol production has been gaining worldwide attention as an alternative to fossil fuel, ethanol productivities and yields are still limited due to the susceptibility of fermentation microorganisms to various stress and inhibitory substances. There is therefore an unmet need to search for multi-stress-tolerant organisms to improve ethanol productivity and reduce production cost, particularly when lignocellulosic hydrolysates are used as the feedstock. RESULTS: Here, we have characterized a previously isolated Pichia kudriavzevii LC375240 strain which is thermotolerant to high temperatures of 37 °C and 42 °C. More excitingly, growth and ethanol productivity of this strain exhibit strong tolerance to multiple stresses such as acetic acid, furfural, formic acid, H(2)O(2) and high concentration of ethanol at 42 °C. In addition, simple immobilization of LC375240 on corncobs resulted to a more stable and higher efficient ethanol production for successive four cycles of repeated batch fermentation at 42 °C. CONCLUSION: The feature of being thermotolerant and multi-stress-tolerant is unique to P. kudriavzevii LC375240 and makes it a good candidate for second-generation bioethanol fermentation as well as for investigating the molecular basis underlying the robust stress tolerance. Immobilization of P. kudriavzevii LC375240 on corncobs is another option for cheap and high ethanol productivity.
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spelling pubmed-72364942020-05-29 Effects of various inhibitory substances and immobilization on ethanol production efficiency of a thermotolerant Pichia kudriavzevii Ndubuisi, Ifeanyi A. Qin, Qijian Liao, Guiyan Wang, Bin Moneke, Anene N. Ogbonna, James C. Jin, Cheng Fang, Wenxia Biotechnol Biofuels Research BACKGROUND: Although bioethanol production has been gaining worldwide attention as an alternative to fossil fuel, ethanol productivities and yields are still limited due to the susceptibility of fermentation microorganisms to various stress and inhibitory substances. There is therefore an unmet need to search for multi-stress-tolerant organisms to improve ethanol productivity and reduce production cost, particularly when lignocellulosic hydrolysates are used as the feedstock. RESULTS: Here, we have characterized a previously isolated Pichia kudriavzevii LC375240 strain which is thermotolerant to high temperatures of 37 °C and 42 °C. More excitingly, growth and ethanol productivity of this strain exhibit strong tolerance to multiple stresses such as acetic acid, furfural, formic acid, H(2)O(2) and high concentration of ethanol at 42 °C. In addition, simple immobilization of LC375240 on corncobs resulted to a more stable and higher efficient ethanol production for successive four cycles of repeated batch fermentation at 42 °C. CONCLUSION: The feature of being thermotolerant and multi-stress-tolerant is unique to P. kudriavzevii LC375240 and makes it a good candidate for second-generation bioethanol fermentation as well as for investigating the molecular basis underlying the robust stress tolerance. Immobilization of P. kudriavzevii LC375240 on corncobs is another option for cheap and high ethanol productivity. BioMed Central 2020-05-18 /pmc/articles/PMC7236494/ /pubmed/32477425 http://dx.doi.org/10.1186/s13068-020-01729-5 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Ndubuisi, Ifeanyi A.
Qin, Qijian
Liao, Guiyan
Wang, Bin
Moneke, Anene N.
Ogbonna, James C.
Jin, Cheng
Fang, Wenxia
Effects of various inhibitory substances and immobilization on ethanol production efficiency of a thermotolerant Pichia kudriavzevii
title Effects of various inhibitory substances and immobilization on ethanol production efficiency of a thermotolerant Pichia kudriavzevii
title_full Effects of various inhibitory substances and immobilization on ethanol production efficiency of a thermotolerant Pichia kudriavzevii
title_fullStr Effects of various inhibitory substances and immobilization on ethanol production efficiency of a thermotolerant Pichia kudriavzevii
title_full_unstemmed Effects of various inhibitory substances and immobilization on ethanol production efficiency of a thermotolerant Pichia kudriavzevii
title_short Effects of various inhibitory substances and immobilization on ethanol production efficiency of a thermotolerant Pichia kudriavzevii
title_sort effects of various inhibitory substances and immobilization on ethanol production efficiency of a thermotolerant pichia kudriavzevii
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7236494/
https://www.ncbi.nlm.nih.gov/pubmed/32477425
http://dx.doi.org/10.1186/s13068-020-01729-5
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