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Deficient Expression of DGCR8 in Human Testis is Related to Spermatogenesis Dysfunction, Especially in Meiosis I

INTRODUCTION: DiGeorge syndrome critical region gene 8 (DGCR8) contributes to miRNA biogenesis, and defects in its expression could lead to defects in spermatogenesis. METHODS: Here, we assess gene and protein expression levels of DGCR8 in the testicular biopsy specimens obtained from men with obstr...

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Autores principales: Babakhanzadeh, Emad, Khodadadian, Ali, Nazari, Majid, Dehghan Tezerjani, Masoud, Aghaei, Seyed Mohsen, Ghasemifar, Sina, Hosseinnia, Mehdi, Mazaheri, Mahta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7237130/
https://www.ncbi.nlm.nih.gov/pubmed/32523370
http://dx.doi.org/10.2147/IJGM.S255431
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author Babakhanzadeh, Emad
Khodadadian, Ali
Nazari, Majid
Dehghan Tezerjani, Masoud
Aghaei, Seyed Mohsen
Ghasemifar, Sina
Hosseinnia, Mehdi
Mazaheri, Mahta
author_facet Babakhanzadeh, Emad
Khodadadian, Ali
Nazari, Majid
Dehghan Tezerjani, Masoud
Aghaei, Seyed Mohsen
Ghasemifar, Sina
Hosseinnia, Mehdi
Mazaheri, Mahta
author_sort Babakhanzadeh, Emad
collection PubMed
description INTRODUCTION: DiGeorge syndrome critical region gene 8 (DGCR8) contributes to miRNA biogenesis, and defects in its expression could lead to defects in spermatogenesis. METHODS: Here, we assess gene and protein expression levels of DGCR8 in the testicular biopsy specimens obtained from men with obstructive azoospermia (OA, n = 19) and various types of non-obstructive azoospermia (NOA) including maturation arrest (MA, n = 17), Sertoli cell-only syndrome (SCOS, n = 20) and hypospermatogenesis (HYPO, 18). Also, samples of men with NOA were divided into two groups based on successful and unsuccessful sperm recovery, NOA+ in 21 patients and NOA− in 34 patients. RESULTS: Examinations disclosed a severe decrease in DGCR8 in samples with MA and SCOS in comparison to OA samples (P < 0.001). Also, the results showed DGCR8 has significantly lower expression in testis tissues of NOA− group in comparison to NOA+ group (p<0.05). Western blot analysis confirmed that the DGCR8 protein was not expressed in SCOS samples and had a very low expression in MA and HYPO samples. DISCUSSION: The results of this survey showed that DGCR8 is an important gene for the entire spermatogenesis pathway. Moreover, DGCR8 gene plays an important role in the diagnosis of NOA subgroups, and also the expression changes in it might contribute to SCOS or MA phenotypes. This gene with considering other related genes can also be a predictor of sperm retrieval.
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spelling pubmed-72371302020-06-09 Deficient Expression of DGCR8 in Human Testis is Related to Spermatogenesis Dysfunction, Especially in Meiosis I Babakhanzadeh, Emad Khodadadian, Ali Nazari, Majid Dehghan Tezerjani, Masoud Aghaei, Seyed Mohsen Ghasemifar, Sina Hosseinnia, Mehdi Mazaheri, Mahta Int J Gen Med Original Research INTRODUCTION: DiGeorge syndrome critical region gene 8 (DGCR8) contributes to miRNA biogenesis, and defects in its expression could lead to defects in spermatogenesis. METHODS: Here, we assess gene and protein expression levels of DGCR8 in the testicular biopsy specimens obtained from men with obstructive azoospermia (OA, n = 19) and various types of non-obstructive azoospermia (NOA) including maturation arrest (MA, n = 17), Sertoli cell-only syndrome (SCOS, n = 20) and hypospermatogenesis (HYPO, 18). Also, samples of men with NOA were divided into two groups based on successful and unsuccessful sperm recovery, NOA+ in 21 patients and NOA− in 34 patients. RESULTS: Examinations disclosed a severe decrease in DGCR8 in samples with MA and SCOS in comparison to OA samples (P < 0.001). Also, the results showed DGCR8 has significantly lower expression in testis tissues of NOA− group in comparison to NOA+ group (p<0.05). Western blot analysis confirmed that the DGCR8 protein was not expressed in SCOS samples and had a very low expression in MA and HYPO samples. DISCUSSION: The results of this survey showed that DGCR8 is an important gene for the entire spermatogenesis pathway. Moreover, DGCR8 gene plays an important role in the diagnosis of NOA subgroups, and also the expression changes in it might contribute to SCOS or MA phenotypes. This gene with considering other related genes can also be a predictor of sperm retrieval. Dove 2020-05-15 /pmc/articles/PMC7237130/ /pubmed/32523370 http://dx.doi.org/10.2147/IJGM.S255431 Text en © 2020 Babakhanzadeh et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Babakhanzadeh, Emad
Khodadadian, Ali
Nazari, Majid
Dehghan Tezerjani, Masoud
Aghaei, Seyed Mohsen
Ghasemifar, Sina
Hosseinnia, Mehdi
Mazaheri, Mahta
Deficient Expression of DGCR8 in Human Testis is Related to Spermatogenesis Dysfunction, Especially in Meiosis I
title Deficient Expression of DGCR8 in Human Testis is Related to Spermatogenesis Dysfunction, Especially in Meiosis I
title_full Deficient Expression of DGCR8 in Human Testis is Related to Spermatogenesis Dysfunction, Especially in Meiosis I
title_fullStr Deficient Expression of DGCR8 in Human Testis is Related to Spermatogenesis Dysfunction, Especially in Meiosis I
title_full_unstemmed Deficient Expression of DGCR8 in Human Testis is Related to Spermatogenesis Dysfunction, Especially in Meiosis I
title_short Deficient Expression of DGCR8 in Human Testis is Related to Spermatogenesis Dysfunction, Especially in Meiosis I
title_sort deficient expression of dgcr8 in human testis is related to spermatogenesis dysfunction, especially in meiosis i
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7237130/
https://www.ncbi.nlm.nih.gov/pubmed/32523370
http://dx.doi.org/10.2147/IJGM.S255431
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