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Eco-Physiological Traits Related to Recovery from Complete Submergence in the Model Legume Lotus japonicus
Submergence is a severe form of stress for most plants. Lotus japonicus is a model legume with potential use in assisting breeding programs of closely related forage Lotus species. Twelve L. japonicus genotypes (10 recombinant inbred lines (RILs) and 2 parental accessions) with different constitutiv...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7238009/ https://www.ncbi.nlm.nih.gov/pubmed/32326202 http://dx.doi.org/10.3390/plants9040538 |
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author | Buraschi, Florencia B. Mollard, Federico P.O. Grimoldi, Agustín A. Striker, Gustavo G. |
author_facet | Buraschi, Florencia B. Mollard, Federico P.O. Grimoldi, Agustín A. Striker, Gustavo G. |
author_sort | Buraschi, Florencia B. |
collection | PubMed |
description | Submergence is a severe form of stress for most plants. Lotus japonicus is a model legume with potential use in assisting breeding programs of closely related forage Lotus species. Twelve L. japonicus genotypes (10 recombinant inbred lines (RILs) and 2 parental accessions) with different constitutive shoot to root dry mass ratios (S:R) were subjected to 7 days of submergence in clear water and allowed to recover for two weeks post-submergence; a set of non-submerged plants served as controls. Relative growth rate (RGR) was used to indicate the recovery ability of the plants. Leaf relative water content (RWC), stomatal conductance (g(s)), greenness of basal and apical leaves, and chlorophyll fluorescence (Fv/Fm, as a measure of photoinhibition) were monitored during recovery, and relationships among these variables and RGR were explored across genotypes. The main results showed (i) variation in recovery ability (RGR) from short-term complete submergence among genotypes, (ii) a trade-off between growth during vs. after the stress indicated by a negative correlation between RGR during submergence and RGR post-submergence, (iii) an inverse relationship between RGR during recovery and S:R upon de-submergence, (iv) positive relationships between RGR at early recovery and RWC and g(s), which were negatively related to S:R, suggesting this parameter as a good estimator of plant water balance post-submergence, (v) chlorophyll retention allowed fast recovery as revealed by the positive relationship between greenness of basal and apical leaves and RGR during the first recovery week, and (vi) full repair of the submergence-damaged photosynthetic apparatus occurred more slowly (second recovery week) than full recovery of plant water relations. The inclusion of these traits contributing to submergence recovery in L. japonicus should be considered to speed up the breeding process of the closely related forage Lotus spp. used in current agriculture. |
format | Online Article Text |
id | pubmed-7238009 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-72380092020-05-28 Eco-Physiological Traits Related to Recovery from Complete Submergence in the Model Legume Lotus japonicus Buraschi, Florencia B. Mollard, Federico P.O. Grimoldi, Agustín A. Striker, Gustavo G. Plants (Basel) Article Submergence is a severe form of stress for most plants. Lotus japonicus is a model legume with potential use in assisting breeding programs of closely related forage Lotus species. Twelve L. japonicus genotypes (10 recombinant inbred lines (RILs) and 2 parental accessions) with different constitutive shoot to root dry mass ratios (S:R) were subjected to 7 days of submergence in clear water and allowed to recover for two weeks post-submergence; a set of non-submerged plants served as controls. Relative growth rate (RGR) was used to indicate the recovery ability of the plants. Leaf relative water content (RWC), stomatal conductance (g(s)), greenness of basal and apical leaves, and chlorophyll fluorescence (Fv/Fm, as a measure of photoinhibition) were monitored during recovery, and relationships among these variables and RGR were explored across genotypes. The main results showed (i) variation in recovery ability (RGR) from short-term complete submergence among genotypes, (ii) a trade-off between growth during vs. after the stress indicated by a negative correlation between RGR during submergence and RGR post-submergence, (iii) an inverse relationship between RGR during recovery and S:R upon de-submergence, (iv) positive relationships between RGR at early recovery and RWC and g(s), which were negatively related to S:R, suggesting this parameter as a good estimator of plant water balance post-submergence, (v) chlorophyll retention allowed fast recovery as revealed by the positive relationship between greenness of basal and apical leaves and RGR during the first recovery week, and (vi) full repair of the submergence-damaged photosynthetic apparatus occurred more slowly (second recovery week) than full recovery of plant water relations. The inclusion of these traits contributing to submergence recovery in L. japonicus should be considered to speed up the breeding process of the closely related forage Lotus spp. used in current agriculture. MDPI 2020-04-21 /pmc/articles/PMC7238009/ /pubmed/32326202 http://dx.doi.org/10.3390/plants9040538 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Buraschi, Florencia B. Mollard, Federico P.O. Grimoldi, Agustín A. Striker, Gustavo G. Eco-Physiological Traits Related to Recovery from Complete Submergence in the Model Legume Lotus japonicus |
title | Eco-Physiological Traits Related to Recovery from Complete Submergence in the Model Legume Lotus japonicus |
title_full | Eco-Physiological Traits Related to Recovery from Complete Submergence in the Model Legume Lotus japonicus |
title_fullStr | Eco-Physiological Traits Related to Recovery from Complete Submergence in the Model Legume Lotus japonicus |
title_full_unstemmed | Eco-Physiological Traits Related to Recovery from Complete Submergence in the Model Legume Lotus japonicus |
title_short | Eco-Physiological Traits Related to Recovery from Complete Submergence in the Model Legume Lotus japonicus |
title_sort | eco-physiological traits related to recovery from complete submergence in the model legume lotus japonicus |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7238009/ https://www.ncbi.nlm.nih.gov/pubmed/32326202 http://dx.doi.org/10.3390/plants9040538 |
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