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Interaction of Lipoplex with Albumin Enhances Gene Expression in Hepatitis Mice

Understanding the in vivo fate of lipoplex, which is composed of cationic liposomes and DNA, is an important issue toward gene therapy. In disease conditions, the fate of lipoplex might change compared with the normal condition. Here, we examined the contribution of interaction with serum components...

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Autores principales: Yoshikawa, Naoki, Fumoto, Shintaro, Yoshikawa, Keiko, Hu, Die, Okami, Kazuya, Kato, Riku, Nakashima, Mikiro, Miyamoto, Hirotaka, Nishida, Koyo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7238045/
https://www.ncbi.nlm.nih.gov/pubmed/32290201
http://dx.doi.org/10.3390/pharmaceutics12040341
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author Yoshikawa, Naoki
Fumoto, Shintaro
Yoshikawa, Keiko
Hu, Die
Okami, Kazuya
Kato, Riku
Nakashima, Mikiro
Miyamoto, Hirotaka
Nishida, Koyo
author_facet Yoshikawa, Naoki
Fumoto, Shintaro
Yoshikawa, Keiko
Hu, Die
Okami, Kazuya
Kato, Riku
Nakashima, Mikiro
Miyamoto, Hirotaka
Nishida, Koyo
author_sort Yoshikawa, Naoki
collection PubMed
description Understanding the in vivo fate of lipoplex, which is composed of cationic liposomes and DNA, is an important issue toward gene therapy. In disease conditions, the fate of lipoplex might change compared with the normal condition. Here, we examined the contribution of interaction with serum components to in vivo transfection using lipoplex in hepatitis mice. Prior to administration, lipoplex was incubated with serum or albumin. In the liver, the interaction with albumin enhanced gene expression in hepatitis mice, while in the lung, the interaction with serum or albumin enhanced it. In normal mice, the interaction with albumin did not enhance hepatic and pulmonary gene expression. Furthermore, hepatic and pulmonary gene expression levels of albumin-interacted lipoplex were correlated with serum transaminases in hepatitis mice. The albumin interaction increased the hepatic accumulation of lipoplex and serum tumor necrosis factor-α level. We suggest that the interaction with albumin enhanced the inflammation level after the administration of lipoplex in hepatitis mice. Consequently, the enhancement of the inflammation level might enhance the gene expression level. Information obtained in the current study will be valuable toward future clinical application of the lipoplex.
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spelling pubmed-72380452020-05-28 Interaction of Lipoplex with Albumin Enhances Gene Expression in Hepatitis Mice Yoshikawa, Naoki Fumoto, Shintaro Yoshikawa, Keiko Hu, Die Okami, Kazuya Kato, Riku Nakashima, Mikiro Miyamoto, Hirotaka Nishida, Koyo Pharmaceutics Article Understanding the in vivo fate of lipoplex, which is composed of cationic liposomes and DNA, is an important issue toward gene therapy. In disease conditions, the fate of lipoplex might change compared with the normal condition. Here, we examined the contribution of interaction with serum components to in vivo transfection using lipoplex in hepatitis mice. Prior to administration, lipoplex was incubated with serum or albumin. In the liver, the interaction with albumin enhanced gene expression in hepatitis mice, while in the lung, the interaction with serum or albumin enhanced it. In normal mice, the interaction with albumin did not enhance hepatic and pulmonary gene expression. Furthermore, hepatic and pulmonary gene expression levels of albumin-interacted lipoplex were correlated with serum transaminases in hepatitis mice. The albumin interaction increased the hepatic accumulation of lipoplex and serum tumor necrosis factor-α level. We suggest that the interaction with albumin enhanced the inflammation level after the administration of lipoplex in hepatitis mice. Consequently, the enhancement of the inflammation level might enhance the gene expression level. Information obtained in the current study will be valuable toward future clinical application of the lipoplex. MDPI 2020-04-10 /pmc/articles/PMC7238045/ /pubmed/32290201 http://dx.doi.org/10.3390/pharmaceutics12040341 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yoshikawa, Naoki
Fumoto, Shintaro
Yoshikawa, Keiko
Hu, Die
Okami, Kazuya
Kato, Riku
Nakashima, Mikiro
Miyamoto, Hirotaka
Nishida, Koyo
Interaction of Lipoplex with Albumin Enhances Gene Expression in Hepatitis Mice
title Interaction of Lipoplex with Albumin Enhances Gene Expression in Hepatitis Mice
title_full Interaction of Lipoplex with Albumin Enhances Gene Expression in Hepatitis Mice
title_fullStr Interaction of Lipoplex with Albumin Enhances Gene Expression in Hepatitis Mice
title_full_unstemmed Interaction of Lipoplex with Albumin Enhances Gene Expression in Hepatitis Mice
title_short Interaction of Lipoplex with Albumin Enhances Gene Expression in Hepatitis Mice
title_sort interaction of lipoplex with albumin enhances gene expression in hepatitis mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7238045/
https://www.ncbi.nlm.nih.gov/pubmed/32290201
http://dx.doi.org/10.3390/pharmaceutics12040341
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