Cargando…

Efficient Micropropagation Protocol for the Conservation of the Endangered Aloe peglerae, an Ornamental and Medicinal Species

A number of Aloe species are facing an extremely high risk of extinction due to habitat loss and over-exploitation for medicinal and ornamental trade. The last global assessment of Aloe peglerae Schönland (in 2003) ranked its global conservation status as ‘endangered’ with a decreasing population tr...

Descripción completa

Detalles Bibliográficos
Autores principales: Hlatshwayo, Nontobeko A., Amoo, Stephen O., Olowoyo, Joshua O., Doležal, Karel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7238064/
https://www.ncbi.nlm.nih.gov/pubmed/32295312
http://dx.doi.org/10.3390/plants9040506
_version_ 1783536457382625280
author Hlatshwayo, Nontobeko A.
Amoo, Stephen O.
Olowoyo, Joshua O.
Doležal, Karel
author_facet Hlatshwayo, Nontobeko A.
Amoo, Stephen O.
Olowoyo, Joshua O.
Doležal, Karel
author_sort Hlatshwayo, Nontobeko A.
collection PubMed
description A number of Aloe species are facing an extremely high risk of extinction due to habitat loss and over-exploitation for medicinal and ornamental trade. The last global assessment of Aloe peglerae Schönland (in 2003) ranked its global conservation status as ‘endangered’ with a decreasing population trend. In the National Red List of South African Plants, the extremely rapid decline of this species has resulted in its conservation status being elevated from ‘endangered’ to ‘critically endangered’ based on recent or new field information. This dramatic decline necessitates the development of a simple, rapid and efficient micropropagation protocol as a conservation measure. An in vitro propagation protocol was therefore established with the regeneration of 12 shoots per shoot-tip explant within 8 weeks using Murashige and Skoog (MS) medium supplemented with 2.5 µM meta-topolin riboside (an aromatic cytokinin). The rooting of the shoots with a 100% frequency on half-strength MS medium without any plant growth resulted in additional six shoots produced per cultured shoot. The resultant plantlets were successfully acclimatized with a 100% survival frequency after 6 weeks. Overall, the developed protocol can result in the production of 3906 transplantable shoots that are ready for rooting per annum from a single shoot-tip explant. It is simple and efficient for seedling production in the ex situ cultivation and conservation of the endangered A. peglerae.
format Online
Article
Text
id pubmed-7238064
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-72380642020-05-28 Efficient Micropropagation Protocol for the Conservation of the Endangered Aloe peglerae, an Ornamental and Medicinal Species Hlatshwayo, Nontobeko A. Amoo, Stephen O. Olowoyo, Joshua O. Doležal, Karel Plants (Basel) Article A number of Aloe species are facing an extremely high risk of extinction due to habitat loss and over-exploitation for medicinal and ornamental trade. The last global assessment of Aloe peglerae Schönland (in 2003) ranked its global conservation status as ‘endangered’ with a decreasing population trend. In the National Red List of South African Plants, the extremely rapid decline of this species has resulted in its conservation status being elevated from ‘endangered’ to ‘critically endangered’ based on recent or new field information. This dramatic decline necessitates the development of a simple, rapid and efficient micropropagation protocol as a conservation measure. An in vitro propagation protocol was therefore established with the regeneration of 12 shoots per shoot-tip explant within 8 weeks using Murashige and Skoog (MS) medium supplemented with 2.5 µM meta-topolin riboside (an aromatic cytokinin). The rooting of the shoots with a 100% frequency on half-strength MS medium without any plant growth resulted in additional six shoots produced per cultured shoot. The resultant plantlets were successfully acclimatized with a 100% survival frequency after 6 weeks. Overall, the developed protocol can result in the production of 3906 transplantable shoots that are ready for rooting per annum from a single shoot-tip explant. It is simple and efficient for seedling production in the ex situ cultivation and conservation of the endangered A. peglerae. MDPI 2020-04-14 /pmc/articles/PMC7238064/ /pubmed/32295312 http://dx.doi.org/10.3390/plants9040506 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hlatshwayo, Nontobeko A.
Amoo, Stephen O.
Olowoyo, Joshua O.
Doležal, Karel
Efficient Micropropagation Protocol for the Conservation of the Endangered Aloe peglerae, an Ornamental and Medicinal Species
title Efficient Micropropagation Protocol for the Conservation of the Endangered Aloe peglerae, an Ornamental and Medicinal Species
title_full Efficient Micropropagation Protocol for the Conservation of the Endangered Aloe peglerae, an Ornamental and Medicinal Species
title_fullStr Efficient Micropropagation Protocol for the Conservation of the Endangered Aloe peglerae, an Ornamental and Medicinal Species
title_full_unstemmed Efficient Micropropagation Protocol for the Conservation of the Endangered Aloe peglerae, an Ornamental and Medicinal Species
title_short Efficient Micropropagation Protocol for the Conservation of the Endangered Aloe peglerae, an Ornamental and Medicinal Species
title_sort efficient micropropagation protocol for the conservation of the endangered aloe peglerae, an ornamental and medicinal species
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7238064/
https://www.ncbi.nlm.nih.gov/pubmed/32295312
http://dx.doi.org/10.3390/plants9040506
work_keys_str_mv AT hlatshwayonontobekoa efficientmicropropagationprotocolfortheconservationoftheendangeredaloepegleraeanornamentalandmedicinalspecies
AT amoostepheno efficientmicropropagationprotocolfortheconservationoftheendangeredaloepegleraeanornamentalandmedicinalspecies
AT olowoyojoshuao efficientmicropropagationprotocolfortheconservationoftheendangeredaloepegleraeanornamentalandmedicinalspecies
AT dolezalkarel efficientmicropropagationprotocolfortheconservationoftheendangeredaloepegleraeanornamentalandmedicinalspecies