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Impact of Bacteriophage-Supplemented Drinking Water on the E. coli Population in the Chicken Gut
Among intestinal coliform microbes in the broiler gut, there are potentially pathogenic Escherichia (E.) coli that can cause avian colibacillosis. The treatment with antibiotics favors the selection of multidrug-resistant bacteria and an alternative to this treatment is urgently required. A chicken...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7238078/ https://www.ncbi.nlm.nih.gov/pubmed/32316373 http://dx.doi.org/10.3390/pathogens9040293 |
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author | Kittler, Sophie Mengden, Ruth Korf, Imke H. E. Bierbrodt, Anna Wittmann, Johannes Plötz, Madeleine Jung, Arne Lehnherr, Tatiana Rohde, Christine Lehnherr, Hansjörg Klein, Günter Kehrenberg, Corinna |
author_facet | Kittler, Sophie Mengden, Ruth Korf, Imke H. E. Bierbrodt, Anna Wittmann, Johannes Plötz, Madeleine Jung, Arne Lehnherr, Tatiana Rohde, Christine Lehnherr, Hansjörg Klein, Günter Kehrenberg, Corinna |
author_sort | Kittler, Sophie |
collection | PubMed |
description | Among intestinal coliform microbes in the broiler gut, there are potentially pathogenic Escherichia (E.) coli that can cause avian colibacillosis. The treatment with antibiotics favors the selection of multidrug-resistant bacteria and an alternative to this treatment is urgently required. A chicken model of intestinal colonization with an apathogenic model strain of E. coli was used to test if oral phage application can prevent or reduce the gut colonization of extraintestinal pathogenic E. coli variants in two individual experiments. The E. coli strain E28 was used as a model strain, which could be differentiated from other E. coli strains colonizing the broiler gut, and was susceptible to all cocktail phages applied. In the first trial, a mixture of six phages was continuously applied via drinking water. No reduction of the model E. coli strain E28 occurred, but phage replication could be demonstrated. In the second trial, the applied mixture was limited to the four phages, which showed highest efficacy in vitro. E. coli colonization was reduced in this trial, but again, no reduction of the E. coli strain E28 was observed. The results of the trials presented here can improve the understanding of the effect of phages on single strains in the multi-strain microbiota of the chicken gut. |
format | Online Article Text |
id | pubmed-7238078 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-72380782020-05-28 Impact of Bacteriophage-Supplemented Drinking Water on the E. coli Population in the Chicken Gut Kittler, Sophie Mengden, Ruth Korf, Imke H. E. Bierbrodt, Anna Wittmann, Johannes Plötz, Madeleine Jung, Arne Lehnherr, Tatiana Rohde, Christine Lehnherr, Hansjörg Klein, Günter Kehrenberg, Corinna Pathogens Article Among intestinal coliform microbes in the broiler gut, there are potentially pathogenic Escherichia (E.) coli that can cause avian colibacillosis. The treatment with antibiotics favors the selection of multidrug-resistant bacteria and an alternative to this treatment is urgently required. A chicken model of intestinal colonization with an apathogenic model strain of E. coli was used to test if oral phage application can prevent or reduce the gut colonization of extraintestinal pathogenic E. coli variants in two individual experiments. The E. coli strain E28 was used as a model strain, which could be differentiated from other E. coli strains colonizing the broiler gut, and was susceptible to all cocktail phages applied. In the first trial, a mixture of six phages was continuously applied via drinking water. No reduction of the model E. coli strain E28 occurred, but phage replication could be demonstrated. In the second trial, the applied mixture was limited to the four phages, which showed highest efficacy in vitro. E. coli colonization was reduced in this trial, but again, no reduction of the E. coli strain E28 was observed. The results of the trials presented here can improve the understanding of the effect of phages on single strains in the multi-strain microbiota of the chicken gut. MDPI 2020-04-16 /pmc/articles/PMC7238078/ /pubmed/32316373 http://dx.doi.org/10.3390/pathogens9040293 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Kittler, Sophie Mengden, Ruth Korf, Imke H. E. Bierbrodt, Anna Wittmann, Johannes Plötz, Madeleine Jung, Arne Lehnherr, Tatiana Rohde, Christine Lehnherr, Hansjörg Klein, Günter Kehrenberg, Corinna Impact of Bacteriophage-Supplemented Drinking Water on the E. coli Population in the Chicken Gut |
title | Impact of Bacteriophage-Supplemented Drinking Water on the E. coli Population in the Chicken Gut |
title_full | Impact of Bacteriophage-Supplemented Drinking Water on the E. coli Population in the Chicken Gut |
title_fullStr | Impact of Bacteriophage-Supplemented Drinking Water on the E. coli Population in the Chicken Gut |
title_full_unstemmed | Impact of Bacteriophage-Supplemented Drinking Water on the E. coli Population in the Chicken Gut |
title_short | Impact of Bacteriophage-Supplemented Drinking Water on the E. coli Population in the Chicken Gut |
title_sort | impact of bacteriophage-supplemented drinking water on the e. coli population in the chicken gut |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7238078/ https://www.ncbi.nlm.nih.gov/pubmed/32316373 http://dx.doi.org/10.3390/pathogens9040293 |
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