Cargando…

Impact of Bacteriophage-Supplemented Drinking Water on the E. coli Population in the Chicken Gut

Among intestinal coliform microbes in the broiler gut, there are potentially pathogenic Escherichia (E.) coli that can cause avian colibacillosis. The treatment with antibiotics favors the selection of multidrug-resistant bacteria and an alternative to this treatment is urgently required. A chicken...

Descripción completa

Detalles Bibliográficos
Autores principales: Kittler, Sophie, Mengden, Ruth, Korf, Imke H. E., Bierbrodt, Anna, Wittmann, Johannes, Plötz, Madeleine, Jung, Arne, Lehnherr, Tatiana, Rohde, Christine, Lehnherr, Hansjörg, Klein, Günter, Kehrenberg, Corinna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7238078/
https://www.ncbi.nlm.nih.gov/pubmed/32316373
http://dx.doi.org/10.3390/pathogens9040293
_version_ 1783536460722339840
author Kittler, Sophie
Mengden, Ruth
Korf, Imke H. E.
Bierbrodt, Anna
Wittmann, Johannes
Plötz, Madeleine
Jung, Arne
Lehnherr, Tatiana
Rohde, Christine
Lehnherr, Hansjörg
Klein, Günter
Kehrenberg, Corinna
author_facet Kittler, Sophie
Mengden, Ruth
Korf, Imke H. E.
Bierbrodt, Anna
Wittmann, Johannes
Plötz, Madeleine
Jung, Arne
Lehnherr, Tatiana
Rohde, Christine
Lehnherr, Hansjörg
Klein, Günter
Kehrenberg, Corinna
author_sort Kittler, Sophie
collection PubMed
description Among intestinal coliform microbes in the broiler gut, there are potentially pathogenic Escherichia (E.) coli that can cause avian colibacillosis. The treatment with antibiotics favors the selection of multidrug-resistant bacteria and an alternative to this treatment is urgently required. A chicken model of intestinal colonization with an apathogenic model strain of E. coli was used to test if oral phage application can prevent or reduce the gut colonization of extraintestinal pathogenic E. coli variants in two individual experiments. The E. coli strain E28 was used as a model strain, which could be differentiated from other E. coli strains colonizing the broiler gut, and was susceptible to all cocktail phages applied. In the first trial, a mixture of six phages was continuously applied via drinking water. No reduction of the model E. coli strain E28 occurred, but phage replication could be demonstrated. In the second trial, the applied mixture was limited to the four phages, which showed highest efficacy in vitro. E. coli colonization was reduced in this trial, but again, no reduction of the E. coli strain E28 was observed. The results of the trials presented here can improve the understanding of the effect of phages on single strains in the multi-strain microbiota of the chicken gut.
format Online
Article
Text
id pubmed-7238078
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-72380782020-05-28 Impact of Bacteriophage-Supplemented Drinking Water on the E. coli Population in the Chicken Gut Kittler, Sophie Mengden, Ruth Korf, Imke H. E. Bierbrodt, Anna Wittmann, Johannes Plötz, Madeleine Jung, Arne Lehnherr, Tatiana Rohde, Christine Lehnherr, Hansjörg Klein, Günter Kehrenberg, Corinna Pathogens Article Among intestinal coliform microbes in the broiler gut, there are potentially pathogenic Escherichia (E.) coli that can cause avian colibacillosis. The treatment with antibiotics favors the selection of multidrug-resistant bacteria and an alternative to this treatment is urgently required. A chicken model of intestinal colonization with an apathogenic model strain of E. coli was used to test if oral phage application can prevent or reduce the gut colonization of extraintestinal pathogenic E. coli variants in two individual experiments. The E. coli strain E28 was used as a model strain, which could be differentiated from other E. coli strains colonizing the broiler gut, and was susceptible to all cocktail phages applied. In the first trial, a mixture of six phages was continuously applied via drinking water. No reduction of the model E. coli strain E28 occurred, but phage replication could be demonstrated. In the second trial, the applied mixture was limited to the four phages, which showed highest efficacy in vitro. E. coli colonization was reduced in this trial, but again, no reduction of the E. coli strain E28 was observed. The results of the trials presented here can improve the understanding of the effect of phages on single strains in the multi-strain microbiota of the chicken gut. MDPI 2020-04-16 /pmc/articles/PMC7238078/ /pubmed/32316373 http://dx.doi.org/10.3390/pathogens9040293 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kittler, Sophie
Mengden, Ruth
Korf, Imke H. E.
Bierbrodt, Anna
Wittmann, Johannes
Plötz, Madeleine
Jung, Arne
Lehnherr, Tatiana
Rohde, Christine
Lehnherr, Hansjörg
Klein, Günter
Kehrenberg, Corinna
Impact of Bacteriophage-Supplemented Drinking Water on the E. coli Population in the Chicken Gut
title Impact of Bacteriophage-Supplemented Drinking Water on the E. coli Population in the Chicken Gut
title_full Impact of Bacteriophage-Supplemented Drinking Water on the E. coli Population in the Chicken Gut
title_fullStr Impact of Bacteriophage-Supplemented Drinking Water on the E. coli Population in the Chicken Gut
title_full_unstemmed Impact of Bacteriophage-Supplemented Drinking Water on the E. coli Population in the Chicken Gut
title_short Impact of Bacteriophage-Supplemented Drinking Water on the E. coli Population in the Chicken Gut
title_sort impact of bacteriophage-supplemented drinking water on the e. coli population in the chicken gut
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7238078/
https://www.ncbi.nlm.nih.gov/pubmed/32316373
http://dx.doi.org/10.3390/pathogens9040293
work_keys_str_mv AT kittlersophie impactofbacteriophagesupplementeddrinkingwaterontheecolipopulationinthechickengut
AT mengdenruth impactofbacteriophagesupplementeddrinkingwaterontheecolipopulationinthechickengut
AT korfimkehe impactofbacteriophagesupplementeddrinkingwaterontheecolipopulationinthechickengut
AT bierbrodtanna impactofbacteriophagesupplementeddrinkingwaterontheecolipopulationinthechickengut
AT wittmannjohannes impactofbacteriophagesupplementeddrinkingwaterontheecolipopulationinthechickengut
AT plotzmadeleine impactofbacteriophagesupplementeddrinkingwaterontheecolipopulationinthechickengut
AT jungarne impactofbacteriophagesupplementeddrinkingwaterontheecolipopulationinthechickengut
AT lehnherrtatiana impactofbacteriophagesupplementeddrinkingwaterontheecolipopulationinthechickengut
AT rohdechristine impactofbacteriophagesupplementeddrinkingwaterontheecolipopulationinthechickengut
AT lehnherrhansjorg impactofbacteriophagesupplementeddrinkingwaterontheecolipopulationinthechickengut
AT kleingunter impactofbacteriophagesupplementeddrinkingwaterontheecolipopulationinthechickengut
AT kehrenbergcorinna impactofbacteriophagesupplementeddrinkingwaterontheecolipopulationinthechickengut