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Polymeric Nanoparticles of Pistacia lentiscus var. chia Essential Oil for Cutaneous Applications

Polymeric nanoparticles (NPs) encapsulating Pistacia lentiscus L. var. chia essential oil (EO) were prepared by a solvent evaporation method, in order to obtain a novel carrier for administration on the skin. The specific EO exhibits antimicrobial and anti-inflammatory properties thus stimulating co...

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Autores principales: Vrouvaki, Ilianna, Koutra, Eleni, Kornaros, Michael, Avgoustakis, Konstantinos, Lamari, Fotini N., Hatziantoniou, Sophia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7238218/
https://www.ncbi.nlm.nih.gov/pubmed/32295134
http://dx.doi.org/10.3390/pharmaceutics12040353
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author Vrouvaki, Ilianna
Koutra, Eleni
Kornaros, Michael
Avgoustakis, Konstantinos
Lamari, Fotini N.
Hatziantoniou, Sophia
author_facet Vrouvaki, Ilianna
Koutra, Eleni
Kornaros, Michael
Avgoustakis, Konstantinos
Lamari, Fotini N.
Hatziantoniou, Sophia
author_sort Vrouvaki, Ilianna
collection PubMed
description Polymeric nanoparticles (NPs) encapsulating Pistacia lentiscus L. var. chia essential oil (EO) were prepared by a solvent evaporation method, in order to obtain a novel carrier for administration on the skin. The specific EO exhibits antimicrobial and anti-inflammatory properties thus stimulating considerable interest as a novel agent for the treatment of minor skin inflammations. The incorporation into nanoparticles could overcome the administration limitations that inserts the nature of the EO. Nanoparticles were prepared, utilizing poly(lactic acid) (PLA) as shell material, due to its biocompatibility and biodegradability, while the influence of surfactant type on NPs properties was examined. Two surfactants were selected, namely poly(vinyl alcohol) (PVA) and lecithin (LEC) and NPs’ physicochemical characteristics i.e. size, polydispersity index (PdI) and ζ-potential were determined, not indicating significant differences (p > 0.05) between PLA/PVA-NPs (239.9 nm, 0.081, -29.1 mV) and PLA/LEC-NPs (286.1 nm, 0.167, −34.5 mV). However, encapsulation efficiency (%EE) measured by GC-MS, was clearly higher for PLA/PVA-NPs than PLA/LEC-NPs (37.45% vs. 9.15%, respectively). Moreover PLA/PVA-NPs remained stable over a period of 60 days. The in vitro release study indicated gradual release of the EO from PLA/PVA-NPs and more immediate from PLA/LEC-NPs. The above findings, in addition to the SEM images of the particles propose a potential structure of nanocapsules for PLA/PVA-NPs, where shell material is mainly consisted of PLA, enclosing the EO in the core. However, this does not seem to be the case for PLA/LEC-NPs, as the results indicated low EO content, rapid release and a considerable percentage of humidity detected by SEM. Furthermore, the Minimum Inhibitory Concentration (MIC) of the EO was determined against Escherichia coli and Bacillus subtilis, while NPs, however did not exhibit considerable activity in the concentration range applied. In conclusion, the surfactant selection may modify the release of EO incorporated in NPs for topical application allowing its action without interfering to the physiological skin microbiota.
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spelling pubmed-72382182020-05-28 Polymeric Nanoparticles of Pistacia lentiscus var. chia Essential Oil for Cutaneous Applications Vrouvaki, Ilianna Koutra, Eleni Kornaros, Michael Avgoustakis, Konstantinos Lamari, Fotini N. Hatziantoniou, Sophia Pharmaceutics Article Polymeric nanoparticles (NPs) encapsulating Pistacia lentiscus L. var. chia essential oil (EO) were prepared by a solvent evaporation method, in order to obtain a novel carrier for administration on the skin. The specific EO exhibits antimicrobial and anti-inflammatory properties thus stimulating considerable interest as a novel agent for the treatment of minor skin inflammations. The incorporation into nanoparticles could overcome the administration limitations that inserts the nature of the EO. Nanoparticles were prepared, utilizing poly(lactic acid) (PLA) as shell material, due to its biocompatibility and biodegradability, while the influence of surfactant type on NPs properties was examined. Two surfactants were selected, namely poly(vinyl alcohol) (PVA) and lecithin (LEC) and NPs’ physicochemical characteristics i.e. size, polydispersity index (PdI) and ζ-potential were determined, not indicating significant differences (p > 0.05) between PLA/PVA-NPs (239.9 nm, 0.081, -29.1 mV) and PLA/LEC-NPs (286.1 nm, 0.167, −34.5 mV). However, encapsulation efficiency (%EE) measured by GC-MS, was clearly higher for PLA/PVA-NPs than PLA/LEC-NPs (37.45% vs. 9.15%, respectively). Moreover PLA/PVA-NPs remained stable over a period of 60 days. The in vitro release study indicated gradual release of the EO from PLA/PVA-NPs and more immediate from PLA/LEC-NPs. The above findings, in addition to the SEM images of the particles propose a potential structure of nanocapsules for PLA/PVA-NPs, where shell material is mainly consisted of PLA, enclosing the EO in the core. However, this does not seem to be the case for PLA/LEC-NPs, as the results indicated low EO content, rapid release and a considerable percentage of humidity detected by SEM. Furthermore, the Minimum Inhibitory Concentration (MIC) of the EO was determined against Escherichia coli and Bacillus subtilis, while NPs, however did not exhibit considerable activity in the concentration range applied. In conclusion, the surfactant selection may modify the release of EO incorporated in NPs for topical application allowing its action without interfering to the physiological skin microbiota. MDPI 2020-04-14 /pmc/articles/PMC7238218/ /pubmed/32295134 http://dx.doi.org/10.3390/pharmaceutics12040353 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Vrouvaki, Ilianna
Koutra, Eleni
Kornaros, Michael
Avgoustakis, Konstantinos
Lamari, Fotini N.
Hatziantoniou, Sophia
Polymeric Nanoparticles of Pistacia lentiscus var. chia Essential Oil for Cutaneous Applications
title Polymeric Nanoparticles of Pistacia lentiscus var. chia Essential Oil for Cutaneous Applications
title_full Polymeric Nanoparticles of Pistacia lentiscus var. chia Essential Oil for Cutaneous Applications
title_fullStr Polymeric Nanoparticles of Pistacia lentiscus var. chia Essential Oil for Cutaneous Applications
title_full_unstemmed Polymeric Nanoparticles of Pistacia lentiscus var. chia Essential Oil for Cutaneous Applications
title_short Polymeric Nanoparticles of Pistacia lentiscus var. chia Essential Oil for Cutaneous Applications
title_sort polymeric nanoparticles of pistacia lentiscus var. chia essential oil for cutaneous applications
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7238218/
https://www.ncbi.nlm.nih.gov/pubmed/32295134
http://dx.doi.org/10.3390/pharmaceutics12040353
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