Cargando…

Shoot Characterization of Isoprene and Ocimene-Emitting Transgenic Arabidopsis Plants under Contrasting Environmental Conditions

Isoprenoids are among the most abundant biogenic volatile compounds (VOCs) emitted by plants, and mediate both biotic and abiotic stress responses. Here, we provide for the first time a comparative analysis of transgenic Arabidopsis lines constitutively emitting isoprene and ocimene. Transgenic line...

Descripción completa

Detalles Bibliográficos
Autores principales: Faralli, Michele, Li, Mingai, Varotto, Claudio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7238224/
https://www.ncbi.nlm.nih.gov/pubmed/32283654
http://dx.doi.org/10.3390/plants9040477
_version_ 1783536496372875264
author Faralli, Michele
Li, Mingai
Varotto, Claudio
author_facet Faralli, Michele
Li, Mingai
Varotto, Claudio
author_sort Faralli, Michele
collection PubMed
description Isoprenoids are among the most abundant biogenic volatile compounds (VOCs) emitted by plants, and mediate both biotic and abiotic stress responses. Here, we provide for the first time a comparative analysis of transgenic Arabidopsis lines constitutively emitting isoprene and ocimene. Transgenic lines and Columbia-0 (Col-0) Arabidopsis were characterized under optimal, water stress, and heat stress conditions. Under optimal conditions, the projected leaf area (PLA), relative growth rate, and final dry weight were generally higher in transgenics than Col-0. These traits were associated to a larger photosynthetic capacity and CO(2) assimilation rate at saturating light. Isoprene and ocimene emitters displayed a moderately higher stress tolerance than Col-0, showing higher PLA and gas-exchange traits throughout the experiments. Contrasting behaviors were recorded for the two overexpressors under water stress, with isoprene emitters showing earlier stomatal closure (conservative behavior) than ocimene emitters (non-conservative behavior), which might suggest different induced strategies for water conservation and stress adaptation. Our work indicates that (i) isoprene and ocimene emitters resulted in enhanced PLA and biomass under optimal and control conditions and that (ii) a moderate stress tolerance is induced when isoprene and ocimene are constitutively emitted in Arabidopsis, thus providing evidence of their role as a potential preferable trait for crop improvement.
format Online
Article
Text
id pubmed-7238224
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-72382242020-05-28 Shoot Characterization of Isoprene and Ocimene-Emitting Transgenic Arabidopsis Plants under Contrasting Environmental Conditions Faralli, Michele Li, Mingai Varotto, Claudio Plants (Basel) Article Isoprenoids are among the most abundant biogenic volatile compounds (VOCs) emitted by plants, and mediate both biotic and abiotic stress responses. Here, we provide for the first time a comparative analysis of transgenic Arabidopsis lines constitutively emitting isoprene and ocimene. Transgenic lines and Columbia-0 (Col-0) Arabidopsis were characterized under optimal, water stress, and heat stress conditions. Under optimal conditions, the projected leaf area (PLA), relative growth rate, and final dry weight were generally higher in transgenics than Col-0. These traits were associated to a larger photosynthetic capacity and CO(2) assimilation rate at saturating light. Isoprene and ocimene emitters displayed a moderately higher stress tolerance than Col-0, showing higher PLA and gas-exchange traits throughout the experiments. Contrasting behaviors were recorded for the two overexpressors under water stress, with isoprene emitters showing earlier stomatal closure (conservative behavior) than ocimene emitters (non-conservative behavior), which might suggest different induced strategies for water conservation and stress adaptation. Our work indicates that (i) isoprene and ocimene emitters resulted in enhanced PLA and biomass under optimal and control conditions and that (ii) a moderate stress tolerance is induced when isoprene and ocimene are constitutively emitted in Arabidopsis, thus providing evidence of their role as a potential preferable trait for crop improvement. MDPI 2020-04-09 /pmc/articles/PMC7238224/ /pubmed/32283654 http://dx.doi.org/10.3390/plants9040477 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Faralli, Michele
Li, Mingai
Varotto, Claudio
Shoot Characterization of Isoprene and Ocimene-Emitting Transgenic Arabidopsis Plants under Contrasting Environmental Conditions
title Shoot Characterization of Isoprene and Ocimene-Emitting Transgenic Arabidopsis Plants under Contrasting Environmental Conditions
title_full Shoot Characterization of Isoprene and Ocimene-Emitting Transgenic Arabidopsis Plants under Contrasting Environmental Conditions
title_fullStr Shoot Characterization of Isoprene and Ocimene-Emitting Transgenic Arabidopsis Plants under Contrasting Environmental Conditions
title_full_unstemmed Shoot Characterization of Isoprene and Ocimene-Emitting Transgenic Arabidopsis Plants under Contrasting Environmental Conditions
title_short Shoot Characterization of Isoprene and Ocimene-Emitting Transgenic Arabidopsis Plants under Contrasting Environmental Conditions
title_sort shoot characterization of isoprene and ocimene-emitting transgenic arabidopsis plants under contrasting environmental conditions
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7238224/
https://www.ncbi.nlm.nih.gov/pubmed/32283654
http://dx.doi.org/10.3390/plants9040477
work_keys_str_mv AT farallimichele shootcharacterizationofisopreneandocimeneemittingtransgenicarabidopsisplantsundercontrastingenvironmentalconditions
AT limingai shootcharacterizationofisopreneandocimeneemittingtransgenicarabidopsisplantsundercontrastingenvironmentalconditions
AT varottoclaudio shootcharacterizationofisopreneandocimeneemittingtransgenicarabidopsisplantsundercontrastingenvironmentalconditions