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Effect of Gold Nanoparticle on 5-Fluorouracil-Induced Experimental Oral Mucositis in Hamsters

Background: Oral mucositis (OM) is a severe inflammation of the oral mucosal cells associated with chemotherapy and/or radiotherapy-induced toxicity, resulting in epithelial ulcers and higher risk of death from sepsis. The aim of the present study was to evaluate the nanoparticle (AuNp) effect on OM...

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Autores principales: Vilar, Carmem Jane Ferreira, Ribeiro, Susana Barbosa, de Araújo, Aurigena Antunes, Guerra, Gerlane Coelho Bernardo, de Araújo Júnior, Raimundo Fernandes, Brito, Gerly Anne de Castro, Leitão, Renata Ferreira Carvalho, Pontes, Daniel de Lima, Gasparotto, Luiz Henrique Da Silva, Oliveira, Maisie Mitchele Barbosa, Viana, Anderson Dias, de Medeiros, Wendy Marina Toscano Queiroz, Bezerra, Breno Gustavo Porfírio, de Medeiros, Caroline Addison Carvalho Xavier
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7238277/
https://www.ncbi.nlm.nih.gov/pubmed/32230975
http://dx.doi.org/10.3390/pharmaceutics12040304
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author Vilar, Carmem Jane Ferreira
Ribeiro, Susana Barbosa
de Araújo, Aurigena Antunes
Guerra, Gerlane Coelho Bernardo
de Araújo Júnior, Raimundo Fernandes
Brito, Gerly Anne de Castro
Leitão, Renata Ferreira Carvalho
Pontes, Daniel de Lima
Gasparotto, Luiz Henrique Da Silva
Oliveira, Maisie Mitchele Barbosa
Viana, Anderson Dias
de Medeiros, Wendy Marina Toscano Queiroz
Bezerra, Breno Gustavo Porfírio
de Medeiros, Caroline Addison Carvalho Xavier
author_facet Vilar, Carmem Jane Ferreira
Ribeiro, Susana Barbosa
de Araújo, Aurigena Antunes
Guerra, Gerlane Coelho Bernardo
de Araújo Júnior, Raimundo Fernandes
Brito, Gerly Anne de Castro
Leitão, Renata Ferreira Carvalho
Pontes, Daniel de Lima
Gasparotto, Luiz Henrique Da Silva
Oliveira, Maisie Mitchele Barbosa
Viana, Anderson Dias
de Medeiros, Wendy Marina Toscano Queiroz
Bezerra, Breno Gustavo Porfírio
de Medeiros, Caroline Addison Carvalho Xavier
author_sort Vilar, Carmem Jane Ferreira
collection PubMed
description Background: Oral mucositis (OM) is a severe inflammation of the oral mucosal cells associated with chemotherapy and/or radiotherapy-induced toxicity, resulting in epithelial ulcers and higher risk of death from sepsis. The aim of the present study was to evaluate the nanoparticle (AuNp) effect on OM induced in hamsters. Materials and methods: 5-fluorouracil (5FU) was used on the first and second day of the experimental model in Golden sirian hamsters, and on the fourth day, mechanical trauma was applied to induce OM. The animals were divided into groups, i.e., polyvinylpyrrolidone (PVP), mechanical trauma (MT), 5FU, and groups treated with gold nanoparticles (AuNps) (62.5, 125, and 250 μg/kg). On the 10th day, animals were euthanized for macroscopic, histopathological, immunohistochemical, western blot, quantitative polymerase chain reaction (qRT-PCR), and AuNp quantification. Results: AuNp (250 μg/kg) reduced TNF-α, IL-1β, COX-2, NF-κB, TGF-β, and SMAD 2/3; increased glutathione levels; decreased the expression of Kelch ECH-associated protein 1 (KEAP1); and induced heme oxygenase 1 (HMOX-1) and NAD (P) H quinone oxidoreductase 1 (NQO1) genes. Conclusions: AuNp (250 μg/kg) prevented 5-FU-induced OM in hamsters and improved the parameters of inflammation and oxidative stress.
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spelling pubmed-72382772020-06-02 Effect of Gold Nanoparticle on 5-Fluorouracil-Induced Experimental Oral Mucositis in Hamsters Vilar, Carmem Jane Ferreira Ribeiro, Susana Barbosa de Araújo, Aurigena Antunes Guerra, Gerlane Coelho Bernardo de Araújo Júnior, Raimundo Fernandes Brito, Gerly Anne de Castro Leitão, Renata Ferreira Carvalho Pontes, Daniel de Lima Gasparotto, Luiz Henrique Da Silva Oliveira, Maisie Mitchele Barbosa Viana, Anderson Dias de Medeiros, Wendy Marina Toscano Queiroz Bezerra, Breno Gustavo Porfírio de Medeiros, Caroline Addison Carvalho Xavier Pharmaceutics Article Background: Oral mucositis (OM) is a severe inflammation of the oral mucosal cells associated with chemotherapy and/or radiotherapy-induced toxicity, resulting in epithelial ulcers and higher risk of death from sepsis. The aim of the present study was to evaluate the nanoparticle (AuNp) effect on OM induced in hamsters. Materials and methods: 5-fluorouracil (5FU) was used on the first and second day of the experimental model in Golden sirian hamsters, and on the fourth day, mechanical trauma was applied to induce OM. The animals were divided into groups, i.e., polyvinylpyrrolidone (PVP), mechanical trauma (MT), 5FU, and groups treated with gold nanoparticles (AuNps) (62.5, 125, and 250 μg/kg). On the 10th day, animals were euthanized for macroscopic, histopathological, immunohistochemical, western blot, quantitative polymerase chain reaction (qRT-PCR), and AuNp quantification. Results: AuNp (250 μg/kg) reduced TNF-α, IL-1β, COX-2, NF-κB, TGF-β, and SMAD 2/3; increased glutathione levels; decreased the expression of Kelch ECH-associated protein 1 (KEAP1); and induced heme oxygenase 1 (HMOX-1) and NAD (P) H quinone oxidoreductase 1 (NQO1) genes. Conclusions: AuNp (250 μg/kg) prevented 5-FU-induced OM in hamsters and improved the parameters of inflammation and oxidative stress. MDPI 2020-03-27 /pmc/articles/PMC7238277/ /pubmed/32230975 http://dx.doi.org/10.3390/pharmaceutics12040304 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Vilar, Carmem Jane Ferreira
Ribeiro, Susana Barbosa
de Araújo, Aurigena Antunes
Guerra, Gerlane Coelho Bernardo
de Araújo Júnior, Raimundo Fernandes
Brito, Gerly Anne de Castro
Leitão, Renata Ferreira Carvalho
Pontes, Daniel de Lima
Gasparotto, Luiz Henrique Da Silva
Oliveira, Maisie Mitchele Barbosa
Viana, Anderson Dias
de Medeiros, Wendy Marina Toscano Queiroz
Bezerra, Breno Gustavo Porfírio
de Medeiros, Caroline Addison Carvalho Xavier
Effect of Gold Nanoparticle on 5-Fluorouracil-Induced Experimental Oral Mucositis in Hamsters
title Effect of Gold Nanoparticle on 5-Fluorouracil-Induced Experimental Oral Mucositis in Hamsters
title_full Effect of Gold Nanoparticle on 5-Fluorouracil-Induced Experimental Oral Mucositis in Hamsters
title_fullStr Effect of Gold Nanoparticle on 5-Fluorouracil-Induced Experimental Oral Mucositis in Hamsters
title_full_unstemmed Effect of Gold Nanoparticle on 5-Fluorouracil-Induced Experimental Oral Mucositis in Hamsters
title_short Effect of Gold Nanoparticle on 5-Fluorouracil-Induced Experimental Oral Mucositis in Hamsters
title_sort effect of gold nanoparticle on 5-fluorouracil-induced experimental oral mucositis in hamsters
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7238277/
https://www.ncbi.nlm.nih.gov/pubmed/32230975
http://dx.doi.org/10.3390/pharmaceutics12040304
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