Cargando…
Styrene Oxide Caused Cell Cycle Arrest and Abolished Myogenic Differentiation of C2C12 Myoblasts
Contaminations of chemicals in foods and drinks are raising public concerns. Among these, styrene, a monomer for plastic production, receives increasing interest due to its ability to leach from the packaging and contaminate in foods and drinks causing many health problems. The present study was des...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7238348/ https://www.ncbi.nlm.nih.gov/pubmed/32454818 http://dx.doi.org/10.1155/2020/1807126 |
_version_ | 1783536522867245056 |
---|---|
author | Surinlert, Piyaporn Kongthong, Nitchamon Watthanard, Mariam Sae-lao, Thannicha Sookbangnop, Piyawat Pholpramool, Chumpol Tipbunjong, Chittipong |
author_facet | Surinlert, Piyaporn Kongthong, Nitchamon Watthanard, Mariam Sae-lao, Thannicha Sookbangnop, Piyawat Pholpramool, Chumpol Tipbunjong, Chittipong |
author_sort | Surinlert, Piyaporn |
collection | PubMed |
description | Contaminations of chemicals in foods and drinks are raising public concerns. Among these, styrene, a monomer for plastic production, receives increasing interest due to its ability to leach from the packaging and contaminate in foods and drinks causing many health problems. The present study was designed to investigate the effects of styrene monomer (STR) and its metabolite styrene oxide (STO) on C2C12 myoblast proliferation and differentiation. Based on an MTT assay, both STR and STO showed no cytotoxic effect at 10–100 μM. However, at 50–100 μM STO, but not STR, significantly inhibited cell proliferation. The STO-treated cells were accumulated in S-phase of cell cycles as revealed by flow cytometry. The antioxidant enzyme (catalase and superoxide dismutase) activities and the gene expressing these enzymes of the arrested cells were decreased and ultimately led to nuclear condensation and expression of apoptotic markers such as cleaved caspase-3 and-9, but not cleaved caspase-8. In addition, STO significantly suppressed myogenic differentiation by decreasing both the number and size of differentiated myotubes. Biochemical analysis showed attenuations of total protein synthesis and myosin heavy chain (MHC) protein expression. In conclusion, a metabolite of styrene, STO, leached from plastic packaging of foods and beverages suppressed both myoblast proliferation and differentiation, which would affect skeletal muscle development and regeneration. |
format | Online Article Text |
id | pubmed-7238348 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-72383482020-05-23 Styrene Oxide Caused Cell Cycle Arrest and Abolished Myogenic Differentiation of C2C12 Myoblasts Surinlert, Piyaporn Kongthong, Nitchamon Watthanard, Mariam Sae-lao, Thannicha Sookbangnop, Piyawat Pholpramool, Chumpol Tipbunjong, Chittipong J Toxicol Research Article Contaminations of chemicals in foods and drinks are raising public concerns. Among these, styrene, a monomer for plastic production, receives increasing interest due to its ability to leach from the packaging and contaminate in foods and drinks causing many health problems. The present study was designed to investigate the effects of styrene monomer (STR) and its metabolite styrene oxide (STO) on C2C12 myoblast proliferation and differentiation. Based on an MTT assay, both STR and STO showed no cytotoxic effect at 10–100 μM. However, at 50–100 μM STO, but not STR, significantly inhibited cell proliferation. The STO-treated cells were accumulated in S-phase of cell cycles as revealed by flow cytometry. The antioxidant enzyme (catalase and superoxide dismutase) activities and the gene expressing these enzymes of the arrested cells were decreased and ultimately led to nuclear condensation and expression of apoptotic markers such as cleaved caspase-3 and-9, but not cleaved caspase-8. In addition, STO significantly suppressed myogenic differentiation by decreasing both the number and size of differentiated myotubes. Biochemical analysis showed attenuations of total protein synthesis and myosin heavy chain (MHC) protein expression. In conclusion, a metabolite of styrene, STO, leached from plastic packaging of foods and beverages suppressed both myoblast proliferation and differentiation, which would affect skeletal muscle development and regeneration. Hindawi 2020-05-11 /pmc/articles/PMC7238348/ /pubmed/32454818 http://dx.doi.org/10.1155/2020/1807126 Text en Copyright © 2020 Piyaporn Surinlert et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Surinlert, Piyaporn Kongthong, Nitchamon Watthanard, Mariam Sae-lao, Thannicha Sookbangnop, Piyawat Pholpramool, Chumpol Tipbunjong, Chittipong Styrene Oxide Caused Cell Cycle Arrest and Abolished Myogenic Differentiation of C2C12 Myoblasts |
title | Styrene Oxide Caused Cell Cycle Arrest and Abolished Myogenic Differentiation of C2C12 Myoblasts |
title_full | Styrene Oxide Caused Cell Cycle Arrest and Abolished Myogenic Differentiation of C2C12 Myoblasts |
title_fullStr | Styrene Oxide Caused Cell Cycle Arrest and Abolished Myogenic Differentiation of C2C12 Myoblasts |
title_full_unstemmed | Styrene Oxide Caused Cell Cycle Arrest and Abolished Myogenic Differentiation of C2C12 Myoblasts |
title_short | Styrene Oxide Caused Cell Cycle Arrest and Abolished Myogenic Differentiation of C2C12 Myoblasts |
title_sort | styrene oxide caused cell cycle arrest and abolished myogenic differentiation of c2c12 myoblasts |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7238348/ https://www.ncbi.nlm.nih.gov/pubmed/32454818 http://dx.doi.org/10.1155/2020/1807126 |
work_keys_str_mv | AT surinlertpiyaporn styreneoxidecausedcellcyclearrestandabolishedmyogenicdifferentiationofc2c12myoblasts AT kongthongnitchamon styreneoxidecausedcellcyclearrestandabolishedmyogenicdifferentiationofc2c12myoblasts AT watthanardmariam styreneoxidecausedcellcyclearrestandabolishedmyogenicdifferentiationofc2c12myoblasts AT saelaothannicha styreneoxidecausedcellcyclearrestandabolishedmyogenicdifferentiationofc2c12myoblasts AT sookbangnoppiyawat styreneoxidecausedcellcyclearrestandabolishedmyogenicdifferentiationofc2c12myoblasts AT pholpramoolchumpol styreneoxidecausedcellcyclearrestandabolishedmyogenicdifferentiationofc2c12myoblasts AT tipbunjongchittipong styreneoxidecausedcellcyclearrestandabolishedmyogenicdifferentiationofc2c12myoblasts |