Cargando…

Quantifying Biophoton Emissions From Human Cells Directly Exposed to Low-Dose Gamma Radiation

Biophoton emission leading to bystander effects (BEs) was shown in beta-irradiated cells; however, technical challenges precluded the analysis of the biophoton role in gamma-induced BEs. The present work was to design an experimental approach to determine if, what type, and how many biophotons could...

Descripción completa

Detalles Bibliográficos
Autores principales: Cohen, Jason, Vo, Nguyen T. K., Chettle, David R., McNeill, Fiona E., Seymour, Colin B., Mothersill, Carmel E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7238447/
https://www.ncbi.nlm.nih.gov/pubmed/32489340
http://dx.doi.org/10.1177/1559325820926763
_version_ 1783536539492417536
author Cohen, Jason
Vo, Nguyen T. K.
Chettle, David R.
McNeill, Fiona E.
Seymour, Colin B.
Mothersill, Carmel E.
author_facet Cohen, Jason
Vo, Nguyen T. K.
Chettle, David R.
McNeill, Fiona E.
Seymour, Colin B.
Mothersill, Carmel E.
author_sort Cohen, Jason
collection PubMed
description Biophoton emission leading to bystander effects (BEs) was shown in beta-irradiated cells; however, technical challenges precluded the analysis of the biophoton role in gamma-induced BEs. The present work was to design an experimental approach to determine if, what type, and how many biophotons could be produced in gamma-irradiated cells. Photon emission was measured in HCT116 p53(+/+) cells irradiated with a total dose of 22 mGy from a cesium-137 source at a dose rate of 45 mGy/min. A single-photon detection unit was used and shielded with lead to reduce counts from stray gammas reaching the detector. Higher quantities of photon emissions were observed when the cells in a tissue culture vessel were present and being irradiated compared to a cell-free vessel. Photon emissions were captured at either 340 nm (in the ultraviolet A [UVA] range) or 610 nm. At the same cell density, radiation exposure time, and radiation dose, HCT116 p53(+/+) cells emitted 2.5 times more UVA biophotons than 610-nm biophotons. For the first time, gamma radiation was shown to induce biophoton emissions from biological cells. As cellular emissions of UVA biophotons following beta radiation lead to BEs, the involvement of cellular emissions of the same type of UVA biophotons in gamma radiation-induced BEs is highly likely.
format Online
Article
Text
id pubmed-7238447
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher SAGE Publications
record_format MEDLINE/PubMed
spelling pubmed-72384472020-06-01 Quantifying Biophoton Emissions From Human Cells Directly Exposed to Low-Dose Gamma Radiation Cohen, Jason Vo, Nguyen T. K. Chettle, David R. McNeill, Fiona E. Seymour, Colin B. Mothersill, Carmel E. Dose Response Original Article Biophoton emission leading to bystander effects (BEs) was shown in beta-irradiated cells; however, technical challenges precluded the analysis of the biophoton role in gamma-induced BEs. The present work was to design an experimental approach to determine if, what type, and how many biophotons could be produced in gamma-irradiated cells. Photon emission was measured in HCT116 p53(+/+) cells irradiated with a total dose of 22 mGy from a cesium-137 source at a dose rate of 45 mGy/min. A single-photon detection unit was used and shielded with lead to reduce counts from stray gammas reaching the detector. Higher quantities of photon emissions were observed when the cells in a tissue culture vessel were present and being irradiated compared to a cell-free vessel. Photon emissions were captured at either 340 nm (in the ultraviolet A [UVA] range) or 610 nm. At the same cell density, radiation exposure time, and radiation dose, HCT116 p53(+/+) cells emitted 2.5 times more UVA biophotons than 610-nm biophotons. For the first time, gamma radiation was shown to induce biophoton emissions from biological cells. As cellular emissions of UVA biophotons following beta radiation lead to BEs, the involvement of cellular emissions of the same type of UVA biophotons in gamma radiation-induced BEs is highly likely. SAGE Publications 2020-05-19 /pmc/articles/PMC7238447/ /pubmed/32489340 http://dx.doi.org/10.1177/1559325820926763 Text en © The Author(s) 2020 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Original Article
Cohen, Jason
Vo, Nguyen T. K.
Chettle, David R.
McNeill, Fiona E.
Seymour, Colin B.
Mothersill, Carmel E.
Quantifying Biophoton Emissions From Human Cells Directly Exposed to Low-Dose Gamma Radiation
title Quantifying Biophoton Emissions From Human Cells Directly Exposed to Low-Dose Gamma Radiation
title_full Quantifying Biophoton Emissions From Human Cells Directly Exposed to Low-Dose Gamma Radiation
title_fullStr Quantifying Biophoton Emissions From Human Cells Directly Exposed to Low-Dose Gamma Radiation
title_full_unstemmed Quantifying Biophoton Emissions From Human Cells Directly Exposed to Low-Dose Gamma Radiation
title_short Quantifying Biophoton Emissions From Human Cells Directly Exposed to Low-Dose Gamma Radiation
title_sort quantifying biophoton emissions from human cells directly exposed to low-dose gamma radiation
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7238447/
https://www.ncbi.nlm.nih.gov/pubmed/32489340
http://dx.doi.org/10.1177/1559325820926763
work_keys_str_mv AT cohenjason quantifyingbiophotonemissionsfromhumancellsdirectlyexposedtolowdosegammaradiation
AT vonguyentk quantifyingbiophotonemissionsfromhumancellsdirectlyexposedtolowdosegammaradiation
AT chettledavidr quantifyingbiophotonemissionsfromhumancellsdirectlyexposedtolowdosegammaradiation
AT mcneillfionae quantifyingbiophotonemissionsfromhumancellsdirectlyexposedtolowdosegammaradiation
AT seymourcolinb quantifyingbiophotonemissionsfromhumancellsdirectlyexposedtolowdosegammaradiation
AT mothersillcarmele quantifyingbiophotonemissionsfromhumancellsdirectlyexposedtolowdosegammaradiation