Rapid Detection of 2019 Novel Coronavirus SARS-CoV-2 Using a CRISPR-based DETECTR Lateral Flow Assay

An outbreak of novel betacoronavirus, SARS-CoV-2 (formerly named 2019-nCoV), began in Wuhan, China in December 2019 and the COVID-19 disease associated with infection has since spread rapidly to multiple countries. Here we report the development of SARS-CoV-2 DETECTR, a rapid (~30 min), low-cost, an...

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Autores principales: Broughton, James P., Deng, Xianding, Yu, Guixia, Fasching, Clare L., Singh, Jasmeet, Streithorst, Jessica, Granados, Andrea, Sotomayor-Gonzalez, Alicia, Zorn, Kelsey, Gopez, Allan, Hsu, Elaine, Gu, Wei, Miller, Steve, Pan, Chao-Yang, Guevara, Hugo, Wadford, Debra A., Chen, Janice S., Chiu, Charles Y.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7239074/
https://www.ncbi.nlm.nih.gov/pubmed/32511449
http://dx.doi.org/10.1101/2020.03.06.20032334
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author Broughton, James P.
Deng, Xianding
Yu, Guixia
Fasching, Clare L.
Singh, Jasmeet
Streithorst, Jessica
Granados, Andrea
Sotomayor-Gonzalez, Alicia
Zorn, Kelsey
Gopez, Allan
Hsu, Elaine
Gu, Wei
Miller, Steve
Pan, Chao-Yang
Guevara, Hugo
Wadford, Debra A.
Chen, Janice S.
Chiu, Charles Y.
author_facet Broughton, James P.
Deng, Xianding
Yu, Guixia
Fasching, Clare L.
Singh, Jasmeet
Streithorst, Jessica
Granados, Andrea
Sotomayor-Gonzalez, Alicia
Zorn, Kelsey
Gopez, Allan
Hsu, Elaine
Gu, Wei
Miller, Steve
Pan, Chao-Yang
Guevara, Hugo
Wadford, Debra A.
Chen, Janice S.
Chiu, Charles Y.
author_sort Broughton, James P.
collection PubMed
description An outbreak of novel betacoronavirus, SARS-CoV-2 (formerly named 2019-nCoV), began in Wuhan, China in December 2019 and the COVID-19 disease associated with infection has since spread rapidly to multiple countries. Here we report the development of SARS-CoV-2 DETECTR, a rapid (~30 min), low-cost, and accurate CRISPR-Cas12 based lateral flow assay for detection of SARS-CoV-2 from respiratory swab RNA extracts. We validated this method using contrived reference samples and clinical samples from infected US patients and demonstrated comparable performance to the US CDC SARS-CoV-2 real-time RT-PCR assay.
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spelling pubmed-72390742020-06-07 Rapid Detection of 2019 Novel Coronavirus SARS-CoV-2 Using a CRISPR-based DETECTR Lateral Flow Assay Broughton, James P. Deng, Xianding Yu, Guixia Fasching, Clare L. Singh, Jasmeet Streithorst, Jessica Granados, Andrea Sotomayor-Gonzalez, Alicia Zorn, Kelsey Gopez, Allan Hsu, Elaine Gu, Wei Miller, Steve Pan, Chao-Yang Guevara, Hugo Wadford, Debra A. Chen, Janice S. Chiu, Charles Y. medRxiv Article An outbreak of novel betacoronavirus, SARS-CoV-2 (formerly named 2019-nCoV), began in Wuhan, China in December 2019 and the COVID-19 disease associated with infection has since spread rapidly to multiple countries. Here we report the development of SARS-CoV-2 DETECTR, a rapid (~30 min), low-cost, and accurate CRISPR-Cas12 based lateral flow assay for detection of SARS-CoV-2 from respiratory swab RNA extracts. We validated this method using contrived reference samples and clinical samples from infected US patients and demonstrated comparable performance to the US CDC SARS-CoV-2 real-time RT-PCR assay. Cold Spring Harbor Laboratory 2020-03-27 /pmc/articles/PMC7239074/ /pubmed/32511449 http://dx.doi.org/10.1101/2020.03.06.20032334 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/It is made available under a CC-BY-NC-ND 4.0 International license (https://creativecommons.org/licenses/by-nc-nd/4.0/) .
spellingShingle Article
Broughton, James P.
Deng, Xianding
Yu, Guixia
Fasching, Clare L.
Singh, Jasmeet
Streithorst, Jessica
Granados, Andrea
Sotomayor-Gonzalez, Alicia
Zorn, Kelsey
Gopez, Allan
Hsu, Elaine
Gu, Wei
Miller, Steve
Pan, Chao-Yang
Guevara, Hugo
Wadford, Debra A.
Chen, Janice S.
Chiu, Charles Y.
Rapid Detection of 2019 Novel Coronavirus SARS-CoV-2 Using a CRISPR-based DETECTR Lateral Flow Assay
title Rapid Detection of 2019 Novel Coronavirus SARS-CoV-2 Using a CRISPR-based DETECTR Lateral Flow Assay
title_full Rapid Detection of 2019 Novel Coronavirus SARS-CoV-2 Using a CRISPR-based DETECTR Lateral Flow Assay
title_fullStr Rapid Detection of 2019 Novel Coronavirus SARS-CoV-2 Using a CRISPR-based DETECTR Lateral Flow Assay
title_full_unstemmed Rapid Detection of 2019 Novel Coronavirus SARS-CoV-2 Using a CRISPR-based DETECTR Lateral Flow Assay
title_short Rapid Detection of 2019 Novel Coronavirus SARS-CoV-2 Using a CRISPR-based DETECTR Lateral Flow Assay
title_sort rapid detection of 2019 novel coronavirus sars-cov-2 using a crispr-based detectr lateral flow assay
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7239074/
https://www.ncbi.nlm.nih.gov/pubmed/32511449
http://dx.doi.org/10.1101/2020.03.06.20032334
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