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A Proposed Method for Simultaneous Measurement of Cuticular Transpiration From Different Leaf Surfaces in Camellia sinensis

The plant cuticle is the major barrier that limits unrestricted water loss and hence plays a critical role in plant drought tolerance. Due to the presence of stomata on the leaf abaxial surface, it is technically challenging to measure abaxial cuticular transpiration. Most of the existing reports we...

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Autores principales: Zhang, Yi, Chen, Xiaobing, Du, Zhenghua, Zhang, Wenjing, Devkota, Ananta Raj, Chen, Zijian, Chen, Changsong, Sun, Weijiang, Chen, Mingjie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7239270/
https://www.ncbi.nlm.nih.gov/pubmed/32477374
http://dx.doi.org/10.3389/fpls.2020.00420
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author Zhang, Yi
Chen, Xiaobing
Du, Zhenghua
Zhang, Wenjing
Devkota, Ananta Raj
Chen, Zijian
Chen, Changsong
Sun, Weijiang
Chen, Mingjie
author_facet Zhang, Yi
Chen, Xiaobing
Du, Zhenghua
Zhang, Wenjing
Devkota, Ananta Raj
Chen, Zijian
Chen, Changsong
Sun, Weijiang
Chen, Mingjie
author_sort Zhang, Yi
collection PubMed
description The plant cuticle is the major barrier that limits unrestricted water loss and hence plays a critical role in plant drought tolerance. Due to the presence of stomata on the leaf abaxial surface, it is technically challenging to measure abaxial cuticular transpiration. Most of the existing reports were only focused on leaf astomatous adaxial surface, and few data are available regarding abaxial cuticular transpiration. Developing a method that can measure cuticular transpiration from both leaf surfaces simultaneously will improve our understanding about leaf transpiration barrier organization. Here, we developed a new method that enabled the simultaneous measurement of cuticular transpiration rates from the adaxial and abaxial surfaces. The proposed method combined multi-step leaf pretreatments including water equilibration under dark and ABA treatment to close stomata, as well as gum arabic or vaseline application to remove or seal the epicuticular wax layer. Mathematical formulas were established and used to calculate the transpiration rates of individual leaf surfaces from observed experimental data. This method facilitates the simultaneous quantification of cuticular transpiration from adaxial and abaxial leaf surfaces. By applying this method, we demonstrated that the adaxial intracuticular waxes and the abaxial epicuticular waxes constitute the major transpiration barriers in Camellia sinensis. Wax analysis indicated that adaxial intracuticular waxes had higher coverage of very long chain fatty acids, 1-alkanol esters, and glycols, which may be attributed to its higher transpiration barrier than that of the abaxial intracuticular waxes.
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spelling pubmed-72392702020-05-29 A Proposed Method for Simultaneous Measurement of Cuticular Transpiration From Different Leaf Surfaces in Camellia sinensis Zhang, Yi Chen, Xiaobing Du, Zhenghua Zhang, Wenjing Devkota, Ananta Raj Chen, Zijian Chen, Changsong Sun, Weijiang Chen, Mingjie Front Plant Sci Plant Science The plant cuticle is the major barrier that limits unrestricted water loss and hence plays a critical role in plant drought tolerance. Due to the presence of stomata on the leaf abaxial surface, it is technically challenging to measure abaxial cuticular transpiration. Most of the existing reports were only focused on leaf astomatous adaxial surface, and few data are available regarding abaxial cuticular transpiration. Developing a method that can measure cuticular transpiration from both leaf surfaces simultaneously will improve our understanding about leaf transpiration barrier organization. Here, we developed a new method that enabled the simultaneous measurement of cuticular transpiration rates from the adaxial and abaxial surfaces. The proposed method combined multi-step leaf pretreatments including water equilibration under dark and ABA treatment to close stomata, as well as gum arabic or vaseline application to remove or seal the epicuticular wax layer. Mathematical formulas were established and used to calculate the transpiration rates of individual leaf surfaces from observed experimental data. This method facilitates the simultaneous quantification of cuticular transpiration from adaxial and abaxial leaf surfaces. By applying this method, we demonstrated that the adaxial intracuticular waxes and the abaxial epicuticular waxes constitute the major transpiration barriers in Camellia sinensis. Wax analysis indicated that adaxial intracuticular waxes had higher coverage of very long chain fatty acids, 1-alkanol esters, and glycols, which may be attributed to its higher transpiration barrier than that of the abaxial intracuticular waxes. Frontiers Media S.A. 2020-05-13 /pmc/articles/PMC7239270/ /pubmed/32477374 http://dx.doi.org/10.3389/fpls.2020.00420 Text en Copyright © 2020 Zhang, Chen, Du, Zhang, Devkota, Chen, Chen, Sun and Chen. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Zhang, Yi
Chen, Xiaobing
Du, Zhenghua
Zhang, Wenjing
Devkota, Ananta Raj
Chen, Zijian
Chen, Changsong
Sun, Weijiang
Chen, Mingjie
A Proposed Method for Simultaneous Measurement of Cuticular Transpiration From Different Leaf Surfaces in Camellia sinensis
title A Proposed Method for Simultaneous Measurement of Cuticular Transpiration From Different Leaf Surfaces in Camellia sinensis
title_full A Proposed Method for Simultaneous Measurement of Cuticular Transpiration From Different Leaf Surfaces in Camellia sinensis
title_fullStr A Proposed Method for Simultaneous Measurement of Cuticular Transpiration From Different Leaf Surfaces in Camellia sinensis
title_full_unstemmed A Proposed Method for Simultaneous Measurement of Cuticular Transpiration From Different Leaf Surfaces in Camellia sinensis
title_short A Proposed Method for Simultaneous Measurement of Cuticular Transpiration From Different Leaf Surfaces in Camellia sinensis
title_sort proposed method for simultaneous measurement of cuticular transpiration from different leaf surfaces in camellia sinensis
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7239270/
https://www.ncbi.nlm.nih.gov/pubmed/32477374
http://dx.doi.org/10.3389/fpls.2020.00420
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